DS 23.219 DYE STAINS OF SPECIAL APPLICATION 491 



23.219 Burdon, Stokes, and Kimbrough 1942 11056, 43:717 



REAGENTS REQUIRED: A. 0.3% Sudan black B; B. 1% safranin 

 method: [emulsify bacteria from slant with .1]-^ make smear—* B, 15 sees. — > wash 



-^ dry 

 RECOMMENDED FOR: demonstration of fat in bacteria. 



23.219 Erb 1929 11284, 14:377 



formula: ether 50, methanol 50, methylene blue 0.59 

 method: [air-dried milk smear] — ♦ stain, 1 min. -^ rinse -^ dry 

 RECOMMENDED FOR: bacteria in milk. 



23.219 Flinn 1939 11284,25:316 



preparation of stain: Grind 0.15 methyl green with 0.5 pyronin and 15 95% ale. 



Add while grinding 85 3% phenol. Leave 1 week. 

 method: [fresh blood smears] — » stain, 4 mins. —* wash -^ dry 

 recommended for: demonstration of bacteria (deep red) in leukocytes. 



23.219 Gay and Clark 1934 11056, 27:175 



REAGENTS REQUIRED: A. DS 11.44 Loffler 1890; B. DS 11.43 Ziehl 1882 



method: [heat-fixed smears] — > A, 3-5 mins. — > rinse -^ B, 5-10 sees. — > wash — > dry 



recommended for: differentiation of living and dead bacteria. 



note: See also DS 23.216 Proca 1909. 



23.219 Kahlden and Laurent 1896 Kahlden and Laurent 1896, 103 



REAGENTS REQUIRED: A. 1% eosiu in 96% ale; B. 1% methyl blue 

 method: [dry smears] -^ A, 1-2 mins. 30°C. —>■ blot -^ B, 30 sees. -* wash -^ dry 

 result: cocci, blue; cellular material, red. 

 RECOMMENDED FOR: differentiation of cocci in smears of cellular material. 



23.219 McCullough and Dick 1942 20540b, 17:153 



formula: water 50, methanol 50, phenol 0.5, sodium chloride 0.5, sodiimi phosphate, 



dibasic (12 H2O) 0.02, methylene blue 0.02 

 method: [smears] -^ methanol, 6 mins. -> stain, 10-30 mins. — > wash — » dry 

 RECOMMENDED FOR: demonstration of bacteria in leukocytes. 



23.219. Schwitz test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 103 



REAGENTS REQUIRED: A. sat. sol. methyl blue in 5% phenol; B. 0.25% acetic acid; C. 



0.2% safranin 

 method: [dried smears] -* A, 5-10 mins. —* B, 2-3 sees. -^ wash -* C, 15-30 mins. -* 



wash — > dry 

 recommended for: differentiation of cocci in smears of cellular material. 



23.219 Shutt 1947 20540b, 22:1 



formula: ether 50, methanol 50, methylene blue 0.5, hydrochloric acid 0.35 

 preparation: Dissolve dye in mixed solvents. Add acid to solution. 

 method: [make smear] -^ stain, 5-10 sees. — > dry 

 recommended for: bacteria in milk. 



23.219 Walton 1939 11284, 24:1308 



formula: water 100, phenol 2, glycerol 20, methanol 10, methyl green 1, pyronin 0.2 

 preparation: Dissolve the dyes in the methanol. Add phenol and water. Shake 2 hours 



per day for 2 days. Filter. Add glycerol to filtrate. 

 method: [heat-fixed smears] -^ stain, on slide, warmed till steaming -^ wash -^ dry 

 result: gonococci, red. 

 recommended for: specific differentiation of gonococcus. 



23.219 Weiss 1929 11284, 16:170 



reagents required: A. water 85, 95%, ale. 10, acetic acid 5, methylene blue 5; B. 1% 



safranin 

 method: [heat-fixed smears] -> A, 5 mins. -^ wash -^ B, ^-2 mins. -> wash —> dry 

 recommended for: demonstration of polar bodies (deep blue) in bacterial cells (red). 



