492 METHODS AND FORMULAS DS 23.219-DS 23.221 



23.219 Zaribnecky 1934 2818, 50:224 



formula: water 90, naphazarine 0.15, azophloxine 0.1 

 preparation: Boil the naphazarine and alum chloride in 60 water. Cool. Filter. Add 



the azophloxine dissolved in 30 water. 

 method: [dried smear of milk] — >• stain, 5-10 mins. —* dry 

 RECOMMENDED FOR: Staining bacteria in milk. 



23.22 BACTERIA IN SECTIONS OF TISSUES 



Relatively few methods are found for staining bacteria in cells for two reasons. First, 

 these methods are rarely, if ever, used for diagnostic purposes but are confined to sec- 

 tions destined for research or teaching; second, the majority of methods which are used 

 for staining bacteria in smears can also be used for staining bacteria in sectioned ma- 

 terial. The methods given below are therefore confined to those which are used exclu- 

 sively for staining sections and which cannot be used for staining smears. For staining 

 sections of specific organisms, for which a technique in smears is recommended, the 

 smear technique should always be tried in preference to one of the techniques here given. 



23.221 General Methods 



23.221 Foshay 1931 11284, 17:193 



formula: water 80, sat. sol. NUe blue sulfate 12, 1% safranin 8 

 method: [sections] — > stain, overnight —^ rinse — > balsam, via usual reagents 



23.221 Fraenkel test. 1928 Schmorl Schmorl 1928, 363 



reagents required: A. DS 11.44 Loffler 1890; B. 0.5% acid fuchsin 30, 33% tannin 30, 



ADS 22.1 Unna (1928) 30 

 method: [sections]—* A, overnight-^ thorough wash — > B, till differentiated —> wash 



-^ balsam, via usual reagents 

 result: bacilli, blue-black; nuclei, light blue; other structures, red. 



23.221 Guyer 1930 Guyer 1930, 117 



REAGENTS REQUIRED: A. DS 11.44 Loffler 1890; B. 0.1% acetic acid 

 method: [sections] — » water — » A, }'2 to 24 hrs. — > B, 10-20 sees. -^ abs. ale, rinse — » 

 balsam, via xylene 



23.221 Holmes and French 1926 see DS 13.21 Holmes and French 1926 



23.221 Krajian 1941 1887a, 32 :825 



REAGENTS REQUIRED: A. DS 11.123 Harris 1900; B. 0.1% hydrochloric acid in 70% ale; 

 C. water 100, zinc sulfate 4, copper sulfate 7; D. 3% brilliant green in C; £^. 5% 

 ammonium nitrate; F. DS 11.43 Ziehl 1882; G. creosote 50, xylene 50 

 method: [7-10 n frozen sections] —* A, 2 mins. -^ tap water, till blue ^ 5, 5 or 6 dips — > 

 C, 3 mins., on slide — > Z), 5 mins., on slide — > rinse -^ E, 1 min. -^ rinse -^ F, 2 mins., 

 on slide — > rinse — > blot — > dioxane, 2 mins. -^ G, till differentiated -^ dammar, via 

 xylene 



23.221 Krajian 1943 11284,28:1602 



reagents required: A. DS 11.44 Loffler 1890; B. creosote 25, xylene 75; C. B 100, 95% 



ale. 5, magenta 1.2 

 method: [sections] -^ water — > A, 3 mins. — » wash — > isopropyl ale, till dehydrated -^ 



B, till differentiated — » C, 1 min. — > B, till excess red removed — * xylene, thorough 



wash -^ balsam 



23.221 Kiihne test. 1904 Besson Besson 1904, 260 



reagents required: A. DS 11.44 Kiihne (1904)a; B. ADS 12.2 Gram 1884; C. sat. ale. 



sol. (circ. 2%) fluorescein 

 method: [sections] -^ water — > A, 5-15 mins. -^ wash — > fi, 2 or 3 mins. — * wash —> C, 



till difl'ercntiated — > balsam, via usual reagents 



23.221 Langeron 1942 see DS 13.22 Langeron 1942b 



