DS 23.222-DS 23.223 DYE STAINS OF SPECIAL APPLICATION 495 



23.222 Mallory 1938 Mallory 1938, 272 



REAGENTS REQLriRED: A. 2.5% phloxinc; B. DP 1 1.45 Sterling 1890; C. ADS 12.2 Gram; 



D. aniline 

 method: [sections of F 3700.0010 Zenker 1894 fixed material, prior stained by DS 1 1.122 

 method] — > A, 10 mins., 50°C. -^ water, wash — > B, 14 to 1 hr. — »• water, wash -^ C, 

 1-2 mins. --> blot -^ D, till differentiated — > balsam, via usual reagents 



23.222 Verchoeff 1940 11006, 115:1546 



REAGENTS REQUIRED: A. DS 11.45 Sterling 1890; B. ADS 12.2 Gram 1880; C. trichlor- 



ethylene; D. oil of thyme 

 method: [sections] — > water —^.4,2 mins. -^ wash — » /?, 1 min. — > 95% ale, till color 

 clouds appear -^ C, till color clouds cease — » D, 1 min. -^ [examine and repeat 95% 

 ale. — > C — » Z) cycle till differentiation complete] — > balsam, via xylene 

 RECOMMENDED FOR: "Lcptotriches " or Parinaud's conjunctives. 



23.222 Weigert 1887 8644, 5 :228 



REAGENTS REQUIRED: A. DS 23.211 Ehrlich 1882; B. 0.9% sodium chloride; C. ADS 12.2 



Gram, 1882; D. aniline. 

 method: [sections] -^ water —> a, 1 min. -^ fi, wash — > C, 1 min. —» blot -^ Z), till 



differentiated -^ xylene -^ balsam 

 note: See comments under "Gram- Weigert" above. 



23.222 Zeissig 1929 20540b, 4:91 



REAGENTS required: .4. DS 23.212 Hucker and Conn 1923; B. ADS 12.2 Gram 1884; C. 



water 95, ADS 12.2 Gram 1884 5 

 method: [sections, with nuclei stained] — > A, 2-3 mins. -^ wash — > B, 5 mins. — > wash 



— > C, till differentiated -^ counterstain, if required -^ balsam, via xylene 



23.223 Methods for Acid-fast Organisms 



Techniques for staining acid-fast organisms in sections essentially follow those for 

 the staining of acid-fast organisms in smears. The most interesting variation is that of 

 Malloiy 1938, in which the acid used is in such a low strength that it permits the 

 retention of hematoxylin stains in the nuclei while still allowing for the differentiation 

 of the bacteria. 



23.223 Adams test. 1946 anonymous 4349, 26:13 



REAGENTS REQUIRED: A. ADS 11.121 Weigert 1903; B. 0.1% hydrochloric acid in 50% 



ale, C. DS 11.43 Goodpasture and Burnett 1919; D. 5% acetic acid; E. 0.1% light 



green in 0.2% acetic acid 

 method: [sections] — > water -^ A,\ min. -^ B, wash — > C, 1 min. —>■ D, till no more color 



comes away — > repeat C -^ D cycle three times — » wash — > ZJ, 1 min. -^85% ale. — > 



balsam, via usual reagents 

 result: nuclei, black; bacteria, red; other structures, green. 



23.223 Albrecht test. 1943 Cowdry Cowdry 1943, 17 



REAGENTS REQUIRED: A. DS 11.43 Albrecht (1943); B. 1% hydrochloric acid in 70% 



ale; C. DS 11.123 Harris 1900; D. 0.3% hydrochloric acid in 70% ale; E. 0.4%, 



ammonium hydroxide 

 method: [sections] — ♦ water — ♦ [lay filter paper saturated with A on slide, steam, 3 mins. 



leave 30 mins.] —>■ B (removing filter paper), till sections deep pink — > wash — > C, 



10 mins. -^ wash — > D, till differentiated -^ wash — ♦ E, till nuclei blue -^ balsam, via 



usual reagents 



23.223 Baumgarten test. 1942 Langeron Langeron 1942, 1212 



REAGENTS REQUIRED: A. DS 11.43 Zichl 1882; B. 10% nitric acid in 95% ale; C. 1% 



toluidine blue 

 method: [sections] — * A, ^^-1 hr. — > water, wash — > B, till no more color comes away 



-^ water, wash — > C, 1-2 mins. — * water, wash — » balsam, via usual reagents 

 RECOMMENDED FOR: Icprosy bacilU in sections. 



