DS 22.223 DYE STAINS OF SPECIAL APPLICATION 497 



23.223 Haythorne 1929 4349, 12:130 



RKAGENTS reqi'iked: .1. 1 )S I 1. 13 Ziclil IS82; li. 10% sulfuric ncid; ('. sat. abs. n\c. sol. 



orange G 

 method: [sections of F 3700.0010 Zenker 1894 fixed material with nuclei hematoxylin 

 stained] ^ water -> .4, 1 hr., 55°C. -^ wash, 20°C. -> wash, 8° to 10°C. -> B, 8-10°C., 

 till sections pale violet -^ wash, 8°-10°C. —> tap water —+95% ale, from drop bottle, 

 least possible time -^ C, from drop bottle, till sections pale orange -^ blot — > balsam, 

 via xylene 



23.223 Krajian 1943 Tech. Bull., 4:45 



RE.\GENTS REQriRED: A. DS 11.43 Ziehl 1882; B. water 40, 95% ale. (iO, arsenic trioxide 



1; C. DS 11.44 Lofiler 1890; D. creosote 50, isopropyl ale. 50 

 method: [frozen sections varnished to slide with celloidin, or mounted paraffin ribbons] 

 — » water —> A, heated to steaming, 3 mins. 



23.223 Kiihne test. 1904 Besson cit. Borrel Besson 1904, 638 



REAGENTS REQUIRED: A. DS 11.122 Bohmer 1868; B. DS 11.43 Ziehl 1890; C. 2% aniline 



hydrochloride 

 method: [sections] -^ water —* a, 2 mins. —» wash -^ 5, 15 niins. ^ C, few sees. — > 



abs. ale, till differentiated -^ balsam, via usual reagents 



23.223 Langeron 1942 Langeron 1942, 1204 



reagents required: A. DS 11.43 Ziehl 1882; B.3% hydrochloric acid; C. 0.4% formal- 

 dehyde; D. water 100, acetic acid 0.6, DS 11.43 Ziehl 1882 0.6; E. water 100, 40% 

 formaldehyde 0.6, acetic acid 0.3; F. DS 12.211 Cajal 1895 

 method: [sections] —> water —> A, }^ to 1 hr. — > water, wash — > B, till no more color 

 comes away — > C, 5 mins. -^ water, wash -^ D, 3 mins. — > water, rinse — ^ E, till no 

 more color comes away —> water, wash — > F, 1 min. — » 95% ale, till dehydrated — > 

 balsam, via usual reagents 



23.223 Langrand 1913 see DS 23.32 Langrand 1913 



23.223 LetuUe 1894 test. 1894 Kahlden and Laurent Kahlden and Laurent 1894, 89 



reagents required: A. DS 11.122 Bohmer 1868; B. DS 11.43 Ziehl 1890; C. water 100, 



iodine green 1, phenol 2 

 method: [sections of F 7000.0000 fixed material]-^ water —> A, 2-3 mins. -^ wash — > 



B, 15 mins. — > rinse — > C, 5 mins. — » abs. ale, till difTerentiated — » balsam, via clove 

 oil 



23.223 Mallory 1938 Mallory 1938, 276 



reagents required: A. DS 11.43 Ziehl 1882; B. 0.1% hydroclJoric acid in 70% ale; 



C. 0.01% ammonia 



method: [sections, nuclei prior stained, by any DS 11.122 method] —> water -^ A, over- 

 night -^ [or, A, 1 hr., 60°C.] -^ B, 20 sees. -♦ C, wash -^ balsam, via usual reagents 

 note: This is specifically recommended for tubercle bacilli. 



23.223 Miiller and Chermock 1945 11284,30:169 



reagents reqi:ired: A. DS 11.43 Miiller and Chermock 1945; B. hydrochloric acid in 



95% ale; C. water 98, 95% ale 2, fast green FCF 0.005. 

 method: [sections] — » water — > A, 5 mins. -^ rinse — » B, till light pink —* C, 2 mins. — > 



rinse — > balsam, via usual reagents 



23.223 Putt 1951 Tech. Bull, 21:94 



REAGENTS REQUIRED: A. water 90, methanol 10, phenol 5, magenta-Ill 1; B. sat. aq. sol. 



lithium carbonate; C. 5% acetic acid in 95% ale; D. 0.5% methylene blue in abs. ale 

 method: [5 M paraffin sections of formaldehyde-fixed material] — + water —* A, 5 mins. —* 



B, 1 min. — > C, 3-5 mins., till sections pale pink — > abs. ale, wash — * D, 1 min. — » abs. 



ale, two changes, 30 sees, each — > neutral mountant, via toluene 

 recommended for: leprosy bacilli. 



