498 METHODS AND FORMULAS DS 23.223-23.224 



23.223 Spoerri 1933 20540b, 23:133 



REAGENTS REQUIRED: A. Water 70, 95% ale. 30, crosyl violet 1, toluidine blue 0.5, 



thionine 0.25; B. 0.2% sulfuric acid 

 method: [4 n paraffin sections attached to slide by V 21.1 Spoerri 1939] -^ water -^ A, 



5-10 sees., 90°C. -^ B, 1 sec. -^ rinse 1 sec. — » 95% ale. 2 sees. -^80% ale, 1 sec. — > 



A, 2 sees.-* [repeat 80% ale. -^ A cycle till stain sufficient] —> balsam, via usual 



reagents 



23.223 Tilden and Tanaka 1945 Tech. Bull, 6 :95 



REAGENTS required: A. DS 11.43 Tilden and Tanaka 1945; B. 40%, neutralized form- 

 aldehyde; C. 1% hydrochloric acid in 70% ale.; D. 1% potassium dichromate; E.2% 

 oxalic acid; F. DS 11.123 Harris 1900; G. DS 12.221 Fite 1939 



method: [sections of formaldehyde-aleohol-fixed material varnished to slide with nitro- 

 ceUulose] —> water — > .4, 15 mins. — > wash — > 5, 5 mins. — > wash -^ C, 5 mins. — » 

 wash —* D, 2-5 mins. —>■ wash -* E, till brown not quite removed — > wash — * F, 2-5 

 mins. -^ wash — > G, 3 rains. — > gum damar, via usual reagents 



23.22Jf. Other Methods for Bacteria in Sections 



Within the present class are grouped all those stains intended for sections which, 

 when used for smears, lie within the classes DS 21.214 through DS 21.218. The ma- 

 jority of smear techniques can, in any case, be used for sections. 



23.224 Lustgarten test. 1903 Cole Cross and Cole 1903, 162 

 REAGENTS REQUIRED: A, aniline 3, 95% ale. 20, crystal violet 1, water 100; B. \% 



potassium permanganate; C. 5% sulfuric acid 

 method: [sections] -^ A, 24 hrs. -^ abs. ak-., till color clouds cease — > B, 10 sees. -+ C, 



few moments -^ wash — > balsam, via usual reagents 

 RECOMMENDED FOR: Spirochetes in sections. 



23.224 Milovidov 1928 see DS 22.21 Milovidov 1928 



23.224 Nikiforoff test. 1916 Warthin 4349, 6 :56 



REAGENTS REQUIRED: A. Water 65, 95% ale. 35, propaeolin 0.35, methylene blue 1.3, 



potassium hydroxide 0.0005; B. abs. ale. 50, ether 50 

 method: [sections of F 3700.0000 Nikiforoff 1916 fixed material] -^ water -^ A, 24 hrs. 



— * wash — * B, few dips -^ balsam, via usual reagents 

 RECOMMENDED FOR: Spirochetes in sections. 



23.224 Schmorl test. 1916 Warthin 4349, 6 :56 



REAGENTS REQUIRED: A. DS 13.13 Gicmsa 1902 3, water 97; B. Q% potassium alum 

 method: [thin frozen sections] -^ A, 1 hr. — > A, fresh solution, 24 hrs. -^ B, wash -^ 



rinse — * [attach to slide, leave tiU nearly dry] -^ cedar oil -^ balsam 

 RECOMMENDED FOR: Spirochetes in sections. 



23.224 Smith test. 1924 Mallory and Wright Mallory and Wright 1924, 289 



REAGENTS REQUIRED: A. DS 23.211 Ehrlich 1882; B. ADS 12.2 Gram 1882; C. 40% form- 

 aldehyde; D. water 100, eosin Y 2.5, methyl green 0.5 

 method: [sections of F 3700.0010-5 Zenker 1894 fixed material] — > A, warmed to steam- 

 ing, few sees.—* drain -^ B, wash — > C, wash ^ 95% ale., till no more color comes 

 away — ♦ B, rinse -^ D, on slide, warmed to steaming, few moments — > water, wash 

 — »• balsam, via usual reagents 

 RECOMMENDED FOR: staining capsules in sections. 



23.224 Stoughton 1930 1025, 17:162 



REAGENTS REQUIRED: A. watcr 100, phcuol 5, thionine 0.1; B. sat. ale. sol. {circ. 0.2%) 



orange G 

 method: [sections] —> water — > ^4, 1 hr. — > abs. ale, till dehydrated -^ B, 1 min. -^ abs. 



ale. till no more color comes away — > balsam, via xylene 

 RECOMMENDED FOR: bacteria in plant tissues. 



