DS 23.31 DYE STAINS OF SPECIAL APPLICATION 501 



high to search for fungal filaments. With- cleared scales in a balsam mount. These 

 out the counterstain it is sometimes very slides may be filed for reference, for they 

 difficult to detect the almost perfectly are quite permanent. 



23.31 PLANTS PARASITIC IN PLANTS 



23.31 Bostr0m test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 120 



REAGENTS REQUIRED: A. DS 23.211 Ehrlich 1882; B. DS 11.24 Ranvier 1889 

 method: [sections] — > water — >• A, 10-15 mins. — > B, 5-10 mins. — ► water, wash -^ abs. 



ale, till differentiated -^ balsam, via xylene 

 RECOMMENDED FOR: Staining actinoniycetes in sections of plant tissues. 



23.31 Cartwright 1929 1032,43:412 



REAGENTS REQUIRED: .1. 1% safranin; B. water 100, anilin blue 1, picric acid 1 

 method: [sections of wood] — > water -^ A, few sees. —>■ water, wash — ► B, heated to sim- 

 mering, few mins. -^ water, till no more color comes away — > balsam, via usual 

 reagents 

 RECOMMENDED FOR: hypliae in wood}^ sections. 



23.31 Cohen 1935 20540h, 10:25 



reagexts required: A. water 97, acetic acid 3, orseillin BB to sat.; B. 1% crystal violet 



in clove oil 

 method: [sections from F 5G00.1000 Cohen 1935 fixed material] — > water ^ ^4, 30 mins. 



— > rinse -^ dehj'drate via graded ales. -^ B, 5 mins. -^ xylene, wash — » balsam 



23.31 Cornwall 1937 Microscope, 1 -AST 



reagents required: .4. water 100, picric acid 0.25, methyl blue 0.35; B. 0.25% safranin 



O in 90% ale. 

 method: [sections of alcohol-fixed wood] — ^ A, 15-20 mins. — » 50% ale, till no more 



color comes away — > B, 5 mins. -^70% ale, rinse — > abs. ale, till pink 

 recommended for: fungus hyphae (blue) in wood. 



23.31 Dickson 1920 19938. 52:63 



reagents required : .4 . 2 % phloxine in 85 % ale ; B. clove oil 98, abs. ale 2, light green 2 

 method: [sections] ^95% ale -^ .4, 5-10 mins. -^95% ale, wash -^ B, 1-3 mins. — » 



clove oil, till no more color comes away — * balsam, via usual reagents 

 recommended for: staining fungus in plant tissues. 

 note: "Phloxine" is given for solution .4 above on the authority of Conn 1946, 112, who 



states that Dickson was in error in supposing his stain to be Magdala red. 



23.31 Ferraril930 2174,2:81 



reagents required: .4. 0.01% ruthenium red; B. 10% potassium hydroxide 

 method: [alcohol-preserved tissues]^ A, till stained (10 mins. to several days) — > B, 



till differentiated —> glycerol 

 recommended for: staining fungi in plant tissues. 



23.31 Garrett 1937 test. 1942 Langeron Langeron 1942, 1285 



formula: water 100, sodium hj'droxide 4, brom thymol blue 0.4 

 method: [fresh root tissue] -^ A, overnight —> glycerol 

 recommended for: staining fungi in roots. 



23.31 Hutchins and Lutman 1941 20540b, 16 :63 



reagents required: .4. water 88, 95% ale 10, aniline 2, crystal violet 5; B. ADS 12.2 



Gram 1884 

 method: [sections] — » water — > .4, 24 hrs. — > wash -^ B, 24 hrs. — > abs. ale, till color 

 clouds cease —* xylene, till no more color comes away — > balsam 

 - recommended for: actinomyces in potato tuber tissue. 



23.31 Israel test. 1940 Johansen Johansen 1940, 226 



reagents reqihred: .4. sat. aq. sol. orcein in 0.1% acetic acid 

 method: [sections] ^ water —> A, several hrs. —> abs. ale, till differentiated (several 



hrs.) —f balsam, via xylene 

 RECOMMENDED FOR: Staining actinoniycetes in sections of plant tissues. 



