502 METHODS AND FORMULAS DS 23.31-DS 23.32 



23.31 Johansen 1940 see DS 13.5 Johansen 1940 



23.31 Langeron 1942 Langeron 1942, 1285 



REAGENTS REQUIRED: A. Water 100, sodium carbonate 20, benzoazurine 0.2; B. glycerol 



90, water 10, cupric sulfate 0.2 

 method: [pieces or sections, chlorine-bleached if necessary] —» A, 1-2 hrs. — » B, for 



preservation 

 RECOMMENDED FOR: Staining fungus in plant tissues. 



23.31 Lepik 1928 16233, 18:869 



REAGENTS REQUIRED: A. 95% alc. 40, phenol 20, lactic acid 40, glycerol 20; B. A (above) 



100, anilin blue 0.02, safranin 0.1; C. DS 12.14 safranin 

 method: [sections] — >■ 95% alc. — > A, 1-15 mins. — » B, 2 hrs. — + A, till differentiated -^ 



abs. alc. till dehydrated — + C, till clear -^ clove oil, till no more color comes away — * 



balsam, via xylene 

 recommended for: staining of Peronosporales in sections. 



23.31 Mangin 1895 5133,8:1 



reagents required: A. 10% potassium hydroxide in 90% alc; B. acetic acid 100, 



orceillin BB q.s.; anilin blue q.s. 

 preparation of B: add enough each of sat. sols, of the two dyes to make a violet mixture. 

 method: [pieces, if necessary chlorine-bleached] -* .4, 1-2 hrs. ^^ 5, till sufficiently 



stained -^ glycerol 

 recommended for: demonstration of Peronosporales in pieces. 



23.31 Margolena 1932 20540b, 7 :25 



reagents required: A. DS 11.44 Stoughton 1930; B. 0.5% light green in 95% alc; 



C. sat. alc. sol. {circ. 0.25%) orange G 30, sat. clove oil sol. erythrosin 60 

 method: [sections] -^ water -^ ^1, 1 hr. -^ water, rinse — > B, till sections appear green 



— > water, till no more color comes away -^ abs. alc, till dehydrated — >, C, 1-2 mins. — > 



balsam, via xylene 

 recommended for: staining fungus hyphae in plant tissues. 

 note: a detailed description of the use of this technique is given under DS 23.30 above. 



23.31 Moore 1933 19938,77:23 



reagents required: A. 2% ferric alum; B. water 20, glycerol 40, lactic acid 20, phenol 

 20, phenosafranin 0.5; C. 0.5% ferric alum in 0.5% hydrochloric acid; D. 1% am- 

 monium hydroxide 

 method: [paraffin sections] — » water —* A, 2 hrs. — > water, quick rinse — > B, 5-15 mins. 

 -^ water, rinse — > C, till differentiated — » D, 15-30 sees. -^ balsam, via usual reagents 

 recommended for: staining hyphae in sections. 



23.31 Ravn test. 1942 Langeron cit. Strasburger Langeron 1942, 1285 



REAGENTS REQUIRED: A. water 100, acetic acid 3, orceillin BB to sat.; B. water 100, 



acetic acid 3, anilin blue to sat. 

 method: [sections of chrome fixed, or mordanted, material] -^ A, 12-24 hrs. — > water, 



wash — * B, 1-2 hrs. -^95% alc, till differentiated — > balsam, via usual reagents 

 recommended for: staining fungus in plant tissues. 



23.31 Vaughn 1914 1048, 1:241 



REAGENTS REQUIRED: A. Water 75, 95% alc. 25, malachite green 0.25, acid fuchsin 0.05, 



Martins yellow 0.005; B. 0.1% hydrochloric acid in 95% alc. 

 method: [sections] -^ water -^ A, K to 1 hr. -^ water, rinse — » B, till differentiated — > 



balsam, via carbol-xylene 

 RECOMMENDED FOR: demonstration of hyphae in sections. 



23.32 PLANTS PARASITIC IN ANIMALS 



23.32 Bachman 1920 1752, 1 :50 



formula: water 70, 95% alc 20, acetic acid 3, orange G 0.5, sat. sol. {circ. 1%) crystal 

 violet 7.5 



