504 METHODS AND FORMULAS DS 23.32 



method: [sections] —> a, 10-15 mins. — > wash -* 5, 10 mins. -^ wash -* C, 1 min. 



wash — ^ balsam, via usual reagents 

 RECOMAiENDED FOR: Saccharomyces in sections. 



23.32 Guegen 1905 5293, 21 :42 



REAGENTS REQUIRED: A. P 12.2 Amann 1896; B. P 12.2 Amann 1896 100, Sudan III to 



sat., aniline blue 0.5 

 PREPARATION OF B: Boil Sudan III in solvent to saturation. Cool. Filter. Dissolve blue 



in filtrate. 

 method: [scrapings] — > A, till clear —> drain -^ B, added on slide -^ cover -^ seal 

 RECOMMENDED FOR: staining fungi in tissue scrapings. 



23.32 Guegen 1906 5293, 22 :224 



formula: lactic acid 100, Sudan III 0.1, auilin blue 0.1, ADS 12.2 Lugol 0.1 

 preparation: Dissolve Sudan III in lactic acid with boiling. Cool. Add other ingredients. 

 method: As Guegen 1905 above. 



23.32 Kligman, Mescon, and DeLameter 1951 Tech. Bull, 21 :86 



reagents required: A. 1% periodic acid; B. DS 11.43 Feulgen and Rosenbeck 1924; 



C. 5% thionyl chloride; D. 1% light green 

 method: [sections] — > water -^ A, 5 mins. -^ thorough wash — > B, 10-15 mins. — > C, 5 



mins. — * thorough wash —^D,l min. — > balsam, via usual reagents 

 note: This is a synthesis of the methods of Hotchkiss 1948 {Arch. Biochem., 16 :131) and 



McManns (20540b, 23:99). 



23.32 Langrand 1913 9775, 7:128 



reagents required: A. DS 11.43 Ziehl 1882; B. 2% aniline hydrochloride; C. DS 12.15 



crystal violet; D. ADS 12.2 Gram 1884; E. 0.2% eosin Y 

 method: [sections]—* A, on slide, warmed to steaming, 5 mins.—* water, wash—* B, 



several dips — * abs. ale, till no more color comes away -^ water, wash — * C, on slide, 



3 mins. -^ water, rinse -^ D, on slide, 1 min. —> water, wash — > E, few sees. — * balsam, 



via usual reagents 

 result: Tubercle bacilli, red; actinomyces, violet. 

 recommended for: demonstration of actinomyces and tubercle bacilli in sections. 



23.32 Lemiere and Becue te.st. 1904 Besson Besson 1904, 677 



reagents required: A. 30% potassium carbonate; B. 5% eosin; C. 50% sodium acetate 

 method: [dry smear] — * ether, 2-3 mins. -^ dry -^ A, several mins. -^ B, 15 mins. — * 



C, 5 mins. -^ seal in C 

 recommended for: actinomyces in smears of pus. 



23.32 Lignieres 1903 1886, 7 :444 



REAGENTS REQUIRED: A. DS 12.15 Crystal violet; B. ADS 12.2 Lugol (1905); C. abs. ale. 

 80, acetone 20, acetic acid 2, sat. sol. {circ. 12.5%) acid fuchsin 0.3; -D. 1% acetic acid 

 method: [sections, nuclei stained by any DS 11.11 technique] -^ water -^ A, 3 mins. -^ 

 rinse -^ B, 1 min. -^ C, till section turns bright red — * water, till differentiation com- 

 plete -^ D, few dips -^ salicylic balsam, via carbol-xylene 

 RECOMMENDED FOR: demonstration of actinomyces in sections. 



23.32 Mahdissan 1935 1798, 85:61 



REAGENTS REQUIRED: A. 5% potassium hydroxide; B. 5% acetic acid; C. 1% acid 

 fuchsin; D. 0.01% picric acid in xylene 



method: [larvae fixed in F 0000.1010 Mahdissan 1935]-^ water -^ A, till skin trans- 

 parent —* B, 5 mins. —> C, 30 mins. — * wash, till no more color comes away — * [graded 

 ales.] -^ D, till clear — * balsam 



recommended for: demonstration of symbiotic microflora in larvae of scale insects. 



23.32 Mallory 1895a lest. 1938 ips. Mallory 1938, 279 



reagents required: A. 2.5% phloxine; B. DS 23.211 Ehrlich 1882; C. ADS 12.2 Gram 



1884; D. aniline 

 method: [paraffin sections of alcohol- or formaldehyde-fixed material, nuclei stained by 

 any DS 11.122 method] -* water -* A, 15 mins., 50°C.^ water, wash^ B, 5-15 mins. 



