DS 23.32 DYE STAINS OF SPECIAL APPLICATION 505 



-^ water, wash — > C, 1 min. — > water, wash -^ blot — > D, till no more color comes 

 away — > balsam, via xylene 

 RECOMMENDED FOR: staining actinomycetes in paraffin sections. 



23.32 Mallory 1895b lest. 1938 ips. Mallory 1938, 279 



REAGENTS REQUIRED: A. DS 23.211 Ehrlich 1882; B. aniline 100, magenta to sat.; C. 



aniline 

 method: [celloidin sections of alcohol- or formaldehyde-fixed material attached to slide 

 after nuclear staining by any DS 11.122 method] — > water — » A, 3-10 mins. — > water, 

 wash -^ blot — > B, 1-3 mins. — > C, till differentiated — > balsam, via xylene 

 recommended for: staining actinomycetes in celloidin sections. 



23.32 Morel and Dulaus tesl. 1904 Besson Besson 1904, 077 



REAGENTS REQUIRED: A. Any DS 11.123 formula; B. 1% Victoria blue in 10% ale; C. 

 ADS 12.2 Gram 1884; D. 1% methyl violet in 10% ale; E. oil of cinnamon 50, abs. 

 ale. 50 

 method: [sections] ^ ^, till nuclei stained —♦ wash —» B, 3 mins. —> wash —> C, few 

 sees. — > 95% ale, wash ^ D, several minutes —> abs. ale. rinse —> E, till differenti- 

 ated —>■ balsam, via usual reagents 

 recommended for: staining actinomyces in sections. 



23.32 Morris test. 1943 Cowdry cit. Mallory and Wright 



Cowdry 1943, 82 

 reagents required: A. 95% ale. 50, ether 50; B. 5% gentian violet in 70% ale; C. 



ADS 12.2 Gram 1882; 1). aniline 100, nitric acid 1 

 method: [fresh scrapings]—* A, 5-10 mins. — > dry -^ B, on slide, 5-30 mins. -^ C, 1 



min. -^ water, rinse —> D, till differentiated —* balsam, via xylene 

 recommended for: staining fimgi in tissue scrapings. 



23.32 Priestly 1917 13034, 2:471 



reagents required: A. P 12.2 Priestly 1917a orb.; B. chloroform; C. formic acid; D. DS 



11.44 Sahli 1885 

 method: [fresh scrapings] —> A, till clear ^ water, wash -^ B, 5 mins. —> strand on 



slide -^ dry -^ C, 2-3 mins., 100°C. -^ water, wash -^ D, 10-15 mins. -^ water, rinse 



-^95% ale, till differentiated -^ balsam, via usual reagents 

 RECOMMENDED FOR: demonstration of hyphae in skin scrapings. 



23.32 Schleifif 1940 14674,87:785 



REAGENTS REQUIRED: .4. F 0000.0010 Camoy 1887; B. 0.5%, azur I 

 method: [skin scrapings on slide] -^ A, 3-10 mins. — > drain -^ dry — > B, on slide, 2-3 



mins. — > wash -^ dry 

 recommended for: demonstration of fungal hyphae in skin. 



23.32 Schubert 1937 7176,105:1025 



reagents required: A. 2% potassium hydroxide; B. water 50, phenol 25, lactic acid 25, 



anilin blue 1.25 

 method: [fresh scrapings] -^ A, }^ to 1 hr. — > water, thorough wash (at least 3 hrs.) —> 



B, on slide — » examine 



recommended for: staining fungi in tissue scrapings. 



23.32 Swartz and Conant 1936 1752, 33:291 



reagents required: A. 10% potassium hydroxide; B. P 12.2 Amann 1890 100, aniliu 



blue 0.5; C. P 12.2 Amann 1896 

 method: [skin scraping]—* A, till clear -^ drain -^ wash, on slide -^ B, 5-10 mins. ^ 



C, till surplus B removed — > M 1 1 movuitant 

 recommended for: demonstration of hyphae in skin scrapings. 



23.32 Unna 1929 7176,88:314 



formula: water 80, glycerol 10, 95% ale 9, pyronin 0.9, methyl green 0.1, phenol 0.5. 

 method: [10 fx sections of alcohol-fixed material]-^ water -^ stain 5-10 sees. -^ water, 



rinse —>■ alis. ale, till doliydratcd -^ balsam, via xylene 

 RECOMMENDED FOR: fungi in skin sections. 



