506 METHODS AND FORMULAS DS 23.33 



23.33 ANIMALS PARASITIC IN ANIMALS 



23.33 Alii 1944 13685, 96:317 



REAGENTS REQUIRED: A. ADS 12.1 All! 1944; B. 4% ferric alum; C. 1% hematein; D. 



0.1% hydrochloric acid in 70% ale. 

 method: [fresh smears] — > A, 10 mins. — > wash — » B, 10 sees. — > rinse — > C, 5-10 mins. 



-^ wash — > D, till differentiated -^ balsam, via usual reagents 

 RECOMMENDED FOR: intestinal protozoans. 

 note: Morrison 1946 substitutes his ADS 12.1 for A and omits B. 



23.33 Anonymous 1946 4349, 26:13 



REAGENTS REQUIRED: A. water 100, calc. chloride 0.01, phloxine 1; B. DS 11.44 Anony- 

 mous 1946; C. acetone; D. 0.2% acetic acid; E. ADS 22.1 Anonymous 1946 



method: [sections] —^ water ^ ^, 5 mins. -^ tap water, 1 min. —^B,l min. — » rinse — > 

 C, 2 mins. — > D, till color clouds cease — > £", 1-2 mins. — > balsam, via acetone and 

 xylene 



RECOMMENDED FOR: malarial parasites in sections. 



23.33 Bidegaray 1926 899a, 4 :385 



REAGENTS REQUIRED: ^. ADS 12.2Lugol (1905) ; B. DS 23.212 NicoUe 1895 (dye solution) 

 method: [fresh fecal smear] — > equal parts of A and B, mixed with smear, 2-3 mins. — > 



examine 

 recommended for: protozoans in fecal smears. 



23.33 Bignami test. 1896 Kahlden and Laurent Kahlden and Laurent 1896, 126 



formula: 95% ale. 100, magenta, aurantia a.a.q.s. to sat. 

 method: [sections of F 3000.0010 Bignami (1896) fixed material]-^ 95% ale.-* stain 



overnight -^ abs. ale, till differentiated — > balsam, via xylene 

 recommended for: Plasmodium in sections. 



23.33 Borrel 1901 857, 15:57 



reagents required: A. sat. aq. sol. magenta; B. DS 12.211 Cajal 1895 



method: [sections of F 1260.0030 Besson 1904 fixed material] -^ water ^ ^, 1 hr. -^ B, 



till differentiated -* abs. ale, minimum possible time — > balsam, via clove oil 

 recommended for: coecidia in tissues. 



23.33 Corbin 1946 Tech. Bull, 7:92 



reagents required: A. water 80; B. 20; B. water 29, glycerol 19 DS 13.13 Giemsa 1902 

 (dry powder) 0.29, DS 11.44 Corbin 1946 3.5, 0.5% eosin Y in 25% ale. 5.7, metha- 

 nol 43 

 method: [air-dried, thick blood smears] -^ A, 2 mins. -^ B, 15 sees. — > wash — * dry 

 recommended for: malarial parasites. 



23.33 Chorine 1932 5310,25:561 



reagents required: A. A.% formaldehyde; pH 7.5; B. water 100, potassium iodide 2, 



ADS 12.2 Lugol (1905) 3; C. 5% sodium thiosulfate; D. water 95, DS 13.13 Giemsa 



1902 5 

 method: [thick smears, even when old] ^ A, till bleached—* water, wash ^ B, 3-5 



mins. -* water, rinse — > C, till colorless -^ water, wash -^ D, 10-30 mins. — >• water 



wash — * dry 

 RECOMMENDED FOR: Staining Plasmodium in smears. 



23.33 Crough and Becker 1931 19938, 73:212 



reagents required: A. acetic acid; B. 0.01% Janus green; C. sat. aq. sol. eosin 

 method: [oocysts, separated by saline flotation, on slide under coverslip] -^ A, drawn 



under cover, 10 mins. 30-40°C. -^ B, drawn under cover, 10 mins. — » C, drawn under 



cover, 5 mins. — > wash — » blot — » seal coverslip 

 recommended for: oocysts of coecidia. 



23.33 Dammin 1937 11284, 23:192 



reagents iiEQi ired: A. water 70, acetic acid 20, 40% formaldehyde 10, picric acid 1; 

 B. 10% sodium hydroxide; C. 5% hydrochloric acid 



