DS 23.33 DYE STAINS OF SPECIAL APPLICATION 507 



method: [short lengths compressed between bound slides]-* A, overnight-* remove 

 slides -> running water, 2-3 mins. -^ B, till partial clearing shows reproductive sys- 

 tem orange on yellow -» water, quick rinse -^ C, 1-2 hrs. -^ water, thorough wash -» 

 balsam, via usual reagents 



RECOMMENDED FOR: diagnostic demonstration of cestode reproductive system. 



23.33 Dobell 1919 Dobell 1919, 7 



REAGENTS REQUIRED: A. watcr 85, 95% ale. 15, acid fuchsin 0.5; B. \% acetic acid; C. 



DS 12.211 Cajal 1895; D. 0.2% acetic acid 

 method: [sections] — > water—* A, 10 mins. -» water, thorough wash -> B, on slide, few 



sees. -^ drain -* C, on slide, 10 mins. -* water, rinse -* D, till differentiated -* abs. 



ale, till differentiated — * balsam, via xylene 

 recommended for: differential staining of E. histolytica in sections. 



23.33 Dobell 1919 see DS 13.7 Dobell 1919 



23.33 DobeU 1942a 16035,34:101 



reagents required: A. 2% phosphotungstic acid; B. 0.2% hematoxyhn 

 method: [fixed smears] -^ A, 10 mins, — » wash -* B, 10 mins. -^ wash -» balsam, via 

 usual reagents 



23.33 Dobelll942b 16035,34:101 



reagents required: A. 2% ammonium molybdate; B. 0.2% hematoxylin 

 method: [fixed smears] — * A, 10 mins. — * thorough wash -> B, 10 mins. -^ wash -^ bal- 

 sam, via usual reagents 

 recommended for: intestinal protozoans, particularly Trichomonas. 



23.33 Field 1940 21671,34:195 



reagents required: A. water 100, sodium phosphate (anhydr.) 1, potassium dihydro- 



gen phosphate (anhydr.) 1.25, brilliant cresyl blue 1 

 method: [air-dried smears] -* A, 1 sec. -^ wash, 5 sees. -^ drain — * dry 

 recommended for: Plasmodium in thick smears. 



23.33 Giemsa 1935 23684, 134:483 



REAGENTS REQUIRED: A. DS 13.13 Giemsa 1902; B. water 100, sodium phosphate, mono- 

 basic 1, eosin Y 0.01 

 method: [dry smear]—* A, 30 mins.-* water, quick rinse—* B, till differentiated—* 



water, rinse —^ dry 

 recommended for: staining Plasmodium in smears. 



23.33 Ginrich 1941 20540b, 16:159 



REAGENTS required: A. DS 13.13 Giemsa 1902 1, water 99; B. DS 13.11 May and 



Griinwald 1902 

 method: [thick blood films] —>■ A, 15 mins. -^ wash -^ dry — * B, 30 sees. —* wash — * dry 

 recommended for: permanent slides of Plasmodium. 



23.33 Goldman 1952 Cowdry 1952 



stock solutions: I. 1 % hematoxylin in 95% ale, II. water 100, sulfuric acid 0.12, acetic 



acid 1, ferric alum 4 

 working solution: stock I 50, stock II 50 



method: [fixed smears] — > stain, H-3 mins. — > wash -^ balsam, via usual reagents 

 RECOMMENDED FOR: intestinal protozoans. 



23.33 Hewitt 1939 11428, 24 (suppl.) :22 



REAGENTS REQUIRED: A. 2.5% potassium dichromate; B. water 97, DS 13.13 Giemsa 



1902 3 

 method: [5 fjt sections of F 3700.1000 Helly 1903 fixed material] -^ water -^ A, K to 1 



hr. -^ water, quick rinse-* B, 24 hrs.-* water, rinse-* 70% ale, till differentiated 



—>■ balsam, via graded acetone-xylene mixtures 

 recommended for: staining Plasmodium in sections. 



