508 METHODS AND FORMULAS DS 23.33 



23.33 Hewitt 1939 600a, 29:115 



REAGENTS hequired: A. 2.5% potassium dichromato; B. water 100, DS 13.13 Giemsa 



2.5, sodium bicarbonate 0.0005 

 method: [5 M sections of F 3700.0010 Zenker 1894 material] -^ water — > A, 30-60 niins. 

 — > rinse — * B, 24 hrs. — > 70% ale, till differentiated — > balsam, via acetone and xylene 

 recommended for: avian malarial parasites in tissues. 



23.33 Hobbs and Thompson 1945 Tech. Bull., 6:29 



REAGENTS REQUIRED: A. watcr 100, potassium dihydrogen phosphate (anhyd.) 1.25, 



sodium phosphate, dibasic (anhyd.) 1, azur B 0.1, methylene blue 0.16; B. 1% eosin 



in same buffer as A. 

 method: [air-dried smear] -^ ^, 1 sec. — > rinse, 5 sees. -^ B,l sec. — ♦ rinse, 5 sees. —> dry 

 RECOMMENDED FOR: Plasmodium. 



23.33 Hollande 1920 1915, 59:75 



REAGENTS REQUIRED: .1. 1 % Bosin Y; B.\% phosphomolybdic acid; C. 0.5% light green; 

 D. amyl ale. 



method: [smears fixed in F 5000.1040 Hollande 1911 or F 4500.1010 Hollande 1918] -> 

 [nuclei stained by any DS 11.111 method and fully differentiated] — ♦ water — > J^^ to 1 

 min. — > 95% ale, till differentiated -^ D, till dehydrated — > balsam, via xylene 



recommended for: staining flagellate protozoans in fecal smears. 



23.33 Jager test. 1928 Schmorl Schmorl 1928, 432 



REAGENTS REQUIRED: A. Any DS 11.122 formula; B. 0.1% eosin Y 

 method: [fecal smears fixed in F 3000.0010 Jager 1928]-^ A, 10 mins. — > wash -^ B, 



1-2 mins. — > balsam, via usual reagents 

 RECOMMENDED FOR: parasitic amebas. 



23.33 Knowles 1931 9943, 64:271 



REAGENTS REQUIRED: A. water 100, acetic acid 1, tartaric acid 0.8; B. methanol; C. 



buffer pH 7.2; D. DS 13.13 Giemsa 1902 

 method: [thick smears] —> A, till bleached-^ water, wash ^^ B, 2-3 mins. — > C, wash 



-^ D, 5-10 mins. — > water, wash -^ dry 



23.33 Kofoid test. 1920 Stitt Stitt 1920, 58 



stock solutions: A. sat. sol. eosin in normal saline; B. sat. sol. iodine in 5% potassium 



iodide in normal saline 

 reagents required: A. stock A 50, stock B 50; ^. normal saline 

 method: Mix 1 drop feces with A, another with B. Place on slide and cover both drops 



with 1 coverslip. 

 recommended for: demonstration of protozoans in fecal smears. 



23.33 Langeron 1942 Langeron 1942, 763 



REAGENTS REQUIRED: .1. DS 11.22 Grenacher 1879; B. water 100, picric acid 1, anilin 



blue 0.6 

 method: [tissues, well washed from fixative]-^ A, 24-48 hrs. -^ 70% ale. thorough 

 wash — > [5 M paraffin sections] -^ water — > B, 12 hrs. -^ water, wash —>■ salicylic bal- 

 sam, via usual reagents 

 recommended for: demonstration of Myxosporid parasites in sections of arthropods. 



23.33 Mallory 1897 test. 1938 ips. Mallory 1938, 297 



REAGENTS required: A. 0.25% thionin; 5. 2% oxalic acid 

 method: [sections] -^ water — > A, ^ to 1 min. -> B, till differentiated }i to 1 min. -♦ 



wash — » balsam, via usual reagents 

 RECOMMENDED FOR: differential staining of Entamoeba in sections. 



23.33 Markey, Culbertson, and Giordano 1943 Tech. Bull., ^:2 



REAGENTS REQUIRED: A. 5% ferric alum; B. water 100, acetic acid 2, hematoxylin 0.1 

 method: [smears fixed in F 3000.0000 Schaudinn 1893] -* water -> A, 2-3 mins. 56°C. 

 — > rinse — > B, 1-2 mins., 56°C. — > running water, 15-30 mins. -> balsam, via usual 

 reagents 

 RECOMMENDED FOR: protozoans in fecal smears. 



