DS 23.33 DYE STAINS OF SPECIAL APPLICATION 509 



23.33 Medalia, Kahaner, and Singer 1944 Tech. Bull., 5 :68 



REAGENTS REQUIRED: A. water 100, sodium phosphate, dibasic 0.56, dihydrogen potas- 

 sium phosphate 1.70; B. water 100, sodium phosphate, dibasic 1.70, dihydrogen po- 

 tassium phosphate 0.5G, methylene blue 0.16, azur II 0.10 

 method: [methanol-fixed smears] — > water — » A, 4 sees. — > rinse — > B, 6 sees. — » rinse — » 



dry 

 RECOMMENDED FORI Plasmodium in smears. 



23.33 Meriwether 1935 4349, 14:64 



RE.\GENTS required: .4.. DS 11.26 Meriwether 1935 24, ammonia 36, methanol 36; A. 



water 45, abs. ale. 36, methanol 18 

 method: [sections of formaldehyde-fixed material with nuclei hematoxylin stained]—* 



water —> A, 5 mins. —> B, till differentiated — * balsam, via usual reagents 

 recommended for: differential staining of parasitic amebas in tissue sections. 



23.33 Moorthy 1937 11428,23:100 



REAGENTS REQUIRED: A. Water 100, sodium chloride 1, mercuric chloride 0.5; B. water 



100, methylene blue 0.06, DS 13.13 Giemsa 1902 (solution) 24 

 method: [larvae of Dracunculus, isolated from Cyclops killed in A]^> ^4, on slide, under 



coverslip — > B, drawn under coverslip —> C, replacing B, drawn under coverslip — > 



[seal coverslip] 

 recommended for: larvae of Dracunculus. 



23.33 Moschkovsky test. 1946 Roskin Roskin 1946, 287 



reagents required: A. water 95, DS 11.44 Moschkovsky (1946) 5; B. 2% tannic acid 

 method: [thick smears] — > A, 10-15 mins. —> rinse -^ B, 1 min. — * rinse -^ dry 

 recommended for: blood parasites in thick smears. 



23.33 Noble 1944 19938, 100:37 



reagents required: A. water 67.5, 40% form.aldehyde 7.5, acetic acid 25, ferric alum 



3; B. 0.5% hematoxylin 

 method: [fresh, moist smear] — > A, on slide, warm to steaming -^> drain — » B, on slide, 



warm to steaming —* wash -^ blot — > balsam, via dioxane and toluene 

 recommended for: protozoan parasites in fecal smears. 



23.33 Reynolds 1936 20540b, 11:167 



formula: water 70, DS 11.21 Guyer 1930 30, DS 11.122 Delafield 1885 10 

 recommended for: nematodes in sections and wholemounts. 



23.33 Rukhadze and Blajin 1929 11587, 32:342 



formula: lactic acid 30, water 100, carmine 0.3 

 preparation: Dissolve with boiling 

 method: [living cestodes] — > stain, }^ to 1 hr. — > running water, 3-^ to 3 hrs. — > balsam, 



via usual reagents 

 recommended for: staining wholemounts of cestodes. 



23.33 Schmorl 1928 Schmorl 1928, 421 



formula: sat. sol. methylene blue 60, 0.5% ethj'l eosin in 70% ale. 20, water 20, 20% 



potassium hydroxide 0.4 

 method: [smears] — > stain, 3-5 mins. — » wash — ► dry 

 recommended for: Plasmodium. 



23.33 Schuffner test. 1928 Schmorl Schmorl 1928, 421 



reagents required: .4. water 94, glycerol 5, 40% formaldehyde 1; B. any DS 11.122 



formula 

 method: [fre.sh blood smear] — ► dry in dark, 6-30 mins. — ♦ A, 5-10 mins. — > wa.sh —* B, 



1-10 mins. -^ wash — >• dry — ► balsam 

 recommended for: Plasmodium. 



