DS 23.34-DS 23.5 DYE STAINS OF SPECIAL APPLICATION 511 



23.34 ANIMALS PARASITIC IN PLANTS 



23.34 Strong 1924 624, 4 :345 



REAGENTS REQUIRED: A. methanol; B. DS 13.13 Giemsa 1902 

 method: [smears of latex, partially dried] — > A, 5 mins. — > water, wash -^ B, on slide, 



10-15 mins. -^ water, wash — » dry 

 RECOMMENDED FOR: demonstration of Phytomonas (the protozoan not the bacteria) in 



Euphorbia. 



23.4 Other Zoological Techniques 



23.5 Other Botanical Techniques 



The techniques given under this heading are so specific for the purpose and class of 

 plant to which they are applied, that they could not be justifiably given under any 

 other heading. These methods, moreover, are so varied that they cannot be subdivided 

 into sections, hence the specific purpose for which each was intended is given under the 

 individual formula. 



23.5 Alcorn and Worley 1936 20540b, 11:119 



REAGENTS REQUIRED: A. DS 11.43 Ziehl 1882; B. 2% hydrochloric acid in 70% ale. 

 method: [dried perithecia scraped from leaf] — * A, 48 hrs. 50°C. -^ [place single perithe- 

 cium on slide, open by tapping coverslip] — * A, under coverslip, 15 mins. 95°C. re- 

 placing stain as it evaporates —> B, drawn under cover, till differentiated -* balsam, 

 via usual reagents 

 recommended for: Perithecia of Erysiphaceae. 



23.5 Baumgartel 1902 1798, 41 :87 



formula: water 99, acetic acid 1, methylene blue 0.5, acid fuchsin q.s. to give violet 



color 

 method : [alcohol-fixed algae] — » stain, till stained — » water, till no more color comes 



away -^ balsam, via usual reagents 

 recommended for: staining wholemounts of algae, particularly Cyanophyceae. 



23.5 Baumgartel 1917 2626, 64:1138 



reagents required: A. water 80, 95% ale. 20, picric acid 0.5, mercuric chloride 1, 



potassium alum 1, hematin 0.1 

 preparation: Dissolve all ingredients except the dye in water. Add dye dissolved in ale. 

 recommended for: staining wholemounts of algae. 



23.5 Bonnet 1910 6630, 38:103 



recommended for: combined fixation and staining of algae. See DS 24 Mennier and 

 Vaney 1910. 



23.5 Chamberlain 1915a Chamberlain 1915, 198 



reagents required: A. 1% eosin Y; B. 1% acetic acid 

 method: [fresh material] -^ abs. ale, 2 mins. — > A, 2 mins. -^ B, quick rinse -^ water, 



thorough wash — > glycerol 

 note: This method is also given by Johansen 1940, 245 without reference to source. 

 recommended for: wholemounts of filamentous fungi. 



23.5 Chamberlain 1915b Chamberlain 1915, 110 



REAGENTS REQUIRED: A. 1% phloxine in 90% ale; B. 1% anilin blue in 90% ale; C. 



0.1% hydrochloric acid in 90% ale. 

 method: [algae in 95% ale] -* A, 24 hrs. -> 90% ale, 1 min. — > B, 3-30 mins. —> C, till 



differentiated — > Venice turpentine, via usual reagents 

 recommended for: wholemounts of filamentous algae. 

 NOTE: A detailed description of the use of this technique is given in Chapter 6. 



