512 METHODS AND FORMULAS DS 23.5 



23.5 Gardiner 1898 test. 1931 Crafts 20540b, 6:127 



REAGEN-TS REQUIRED: A. watcr 100, iodiue 0.5, potassium iodide 0.75; B. 10% sulfuric 

 acid; C. water 95, sulfuric acid 5, iodine 1, potassium diodide 1.25; D. 5% sulfuric 

 acid E. water 95, sulfuric acid 5, 0.5% gentian violet q.s. to give green color. 

 method: [sections of living material] ^ ^, 5 mins. -^ B, 5 mins. -^ C, 5 mins. — » D, till 



iodine starts to fade —>■ E, till dark -^ P 1,3.1 Gardiner 1898 

 RECOMMENDED FOR: demonstration of protoplasmic connections between plant cells. 



23.5 Gutstein 1924 23684, 93 :233 



REAGENTS REQUIRED: A. 1% methylene blue; B. 5% tannic acid; C. 1% safranin 

 method: [heat-dried smear] ^^, 2-3 mins. ^ water, wash -^ B, 2 mins. —> water, 



wash — > C, 2 mins. -^ water, wash -^ dry 

 recommended for: demonstration of nuclei in yeasts. 



23.5 Gutstein 1925 23684, 95:1 



REAGENTS REQUIRED: A. DS 11.43 Ziehl 1882; B. 5% acetic acid; C. 5% tannic acid; 



D. 1% safranin 

 method: [heat-dried smears]-^ A, 2 mins.—* B, till no more color comes away-* 



water, wash -^ C, 2 mins. -^ water, wash -^ D, 2-5 mins. -^ water, wash — » dry 

 recommended for: demonstration of ascospores in yeast. 



23.5 Johansen 1940 Johansen 1940, 270 



REAGENTS REQUIRED: A. 1% Bismarck brown in 70% ale; B. DS 12.16 Johansen 1940; 



C. clove oil 50, abs. ale. 25, xylene 25 

 method: [sections] — > water -^ A, 20 mins. — > 95% ale, till no more color comes away 



-^ B, 5-8 sees. — * C, till differentiated -^ balsam, via xylene 

 recommended for: staining sections of Thallophyta. 



23.5 Kufferath 1929 test. 1942 Langeron Langeron 1942, 1249 



reagents required: .4. DS 11.43 Ziehl 1882; B. 2% lactic acid in 95%, ale; C. 1% Nile 



blue sulfate 

 method: [smear, dried at 100°C.] — > A, on slide, warmed nearly to boiling—* drain —> 



water, wash -^ B, & few sees. — * water, wash -h> C, 30 sees. -^ water, wash -^ dry 

 RECOMMENDED FOR: staining ascospores in yeasts. 



23.5 Langeron 1942 Langeron 1942, 1239 



formula: water 100, acetic acid 3, anilin blue 0.5 

 method: [dried hyphae on slide] ^ stain, 5 mins. -^ water, till no more color comes 



away -^ balsam, via usual reagents 

 RECOMMENDED FOR: Staining fungus mycelia. 



23.5 Maneval 1929 20540b, 4:21 



REAGENTS REQUIRED: A. 1% acid fuchsin; B. 5% tannic acid; C. 2% sulfuric acid 

 method: [heat-dried smears] — » A, 1 min. -^ B, 20 sees. — > C, till differentiated — > bal- 

 sam, via usual reagents 

 recommended for: demonstration of nuclei in yeasts. 



23.5 Rivalier and Seydel 1932 6630, 110:181 



formula: a. P 12.2 Amann 1896 100, anilin blue 1 

 method: [collodion varnished coverslip preparations] —* stain, 10-50 mins. -^70% ale, 



rinse—* 90% ale, till differentiated -*• balsam, via graded acetone-xylene series 

 RECOMMENDED FOR: staining fungus mycelium in coverslip cultures. 



23.5 Semmens 1937 Microscope, 1 :5 



REAGENTS REQUIRED: A . P 12.2 Scmmcns 1937 100, osmic acid 0.04 and either light green 



0.5 or anilin blue 0.5 or phloxine 0.5; B. P 12.2 Semmens 1937 

 method: [fresh algae] -^ .4, 1 hr. — * A, another color, if required, 5-30 mins. — + B, till 



required — > B, on slide -^ ring cover with V 11.2 Semmens 1937 

 RECOMMENDED FOR: wholemouiits of algae. 



