DS 23.5-DS 24 DYE STAINS OF SPECIAL APPLICATION 513 



23.5 Taylor 1921 21400a, 40:94 



REAGENTS REQUIRED: A. 0.05% methylene blue; B. 0.1% picric acid 

 method: [fresh material] -^ A, '2 to 1 min. -^ water, rinse —> B —* examine 

 RECOMMENDED FOR: sheath structure of desmids. 



23.5 Yamanouchi test. 1915 Chamberlain Chamberlain 1915, 167 



REAGENTS REQUIRED: A. 10% alc; B. 1% safranin in 95% ale; C. 1% crystal violet; D. 



1% orange G 

 method: [dried smears] — > A, overnight —* B, i days -^ water, 5 mins. -^ C, 2 days —> 



— > water, brief rinse — > D, 3 niins. — > 95% ale, brief rinse —> abs. alc. 1 min. —^ clove 



oil, till differentiated —^ balsam, via xylene 

 RECOMMENDED FOR: wholcmounts of small Chlorophyceae. 



24 MISCELLANEOUS DYE-STAINING TECHNIQUES 



The existence of any techniques in the present miscellaneous class must of necessity be a 

 criticism of the classification adopted by the author. He is, therefore, pleased that few tech- 

 niques have so far evaded the classification which he has developed as to be forced into the 

 refuge of this miscellaneous group. 



24 Francotte test. 1942 Langeron Langeron 1942, 1011 



formula: water 55, 90% alc. 20, glycerol 20, 40% formaldehyde 5, Bismarck brown 



0.05, malachite green 0.10 

 method: Fresh or preserved plankton is mixed with stain and examined. For perma- 

 nence allow to evaporate under coverslip and replace with P 12.1 Francotte (1942). 

 recommended for: staining mixed plankton collections. 



24 Langeron 1942 Langeron 1942, 8GG 



REAGENTS REQUIRED: A. 5% potassium hydroxide; B. 1% acetic acid; C. any DS 11.24 



formula; D. 1% anilin blue in 1% acetic acid 

 method: [roughly dissected radulae] -^ .4, boiling, till clear —> water, rinse —> B, till 



neutralized — » C, >^ to 1 hr. -^ water, wash — * D, few mins. -^ balsam, via usual 



reagents 

 recommended for: demonstration of radula of moUusca. 



24 Meunier and Vaney 1910 6630, 68:727 



reagents required: A. water 100, quinone 0.3 

 .method: [fresh plankton] -^ A, equal vol., till objects settled out — » 70% alc, 2 changes 



—> balsam, via usual reagents 

 note: An identical technique was applied to algae by Bonnet 1910 (6630, 38). 

 recommended for: staining mixed plankton collections. 



