22 



Accessory Dye-staining Solutions 



Decimal Divisions Used in Chapter 



ADS 10 MORDANTS AND MISCELLANEOUS SOLUTIONS 



1 1 Miscellaneous 

 ILl Formulas 



12 Mordants 



12.1 Formulas for use before hematoxylin 



12.2 Formulas for use before other dyes 

 ADS 20 DIFFERENTIATING SOLUTIONS 



21 For differentiating hematoxylin 

 21.1 Formulas 



22 For other purposes 

 22.1 Formulas 



ADS 10 Mordants and Miscellaneous Solutions 



These formulas have been separated from the stains with which they are custom- 

 arily associated because many of them are capable of a much wider employment than 

 they usually receive. One might take, for example, the mordant of Casares-Gil (ADS 

 12.2) which was originally developed for staining the flagella of bacteria, but which 

 can be applied admirably to revive the staining properties of sections of tissues which 

 have been preserved so long in alcohol that they would otherwise be useless. Attention 

 should also be paid to such formulas as those of Drew 1920 (ADS 12.1) which, by 

 combining a chromic-acid and an osmic-acid mordant, enable one to apply stains, usu- 

 ally appHed only to tissues fixed in such fluids, to tissues which have been fixed in picric 

 acid, and which have therefore been rendered incapable of giving a good differentiation 

 with many of the common triple staining methods. 



11 MISCELLANEOUS 



These formulas are of very wide application, and are accordingly placed before the 

 formulas of specific application. 



11.1 Formulas 



11.1 Anderson 1929 Anderson 1929, 127 



formula: water 100, potassium dichromate .375, calcium hypochlorite 1.25 

 preparation: Dissolve the dichromate and hypochlorite in separate portions of the 



water. Filter the hypochlorite into the dichromate. 

 use: After osmic stains to prevent removal of alcohol-soluble fats. 



11.1 Chura 1925 23632, 42:59 



formula: water 70, acetic acid 30, picric acid q.s. to sat. 

 RECOMMENDED FOR: solution of Cytoplasmic inclusions in chrome-fixed material to 



render chromosomes more evident. 

 note: See also ADS 12.1 Chura 1925. 



514 



