ADS 11.1-ADS 12.1 ACCESSORY DYE-STAINING SOLUTIONS 515 



note: The proportions of the above mixture are misquoted by Minouchi 1928 (see ADS 

 12.1). Gatenby and Painter 1937, 267 assign Minouchi's formula to Chura but give 

 the wrong reference. 



11.1 Hotchkiss 1948 Arch. Biochem., 16:131 



REAGENTS REQUIRED: A. Water 30, abs. ale. 70, sodium acetate 0.8, periodic acid 0.8; 



B. water 40, abs. ale. 60, sodium thiosulfate, cryst 2, potassium iodide 2, hydrochloric 



acid 0.07 

 method: [sections]— > water-* A, 2-5 mins. -^ 70% ale, rinse—* B, 1 min. -* 70% 



ale, wash 

 recommended for: originally intended for increasing stainability of polysaccharides. 



Now recommended for many other purposes. 



11.1 Szecsi 1913 7276,39:1584 



FORMUL.\: acetone 100, benzyol peroxide 10 

 recommended for: use as a tissue reviver. 



12 MORDANTS 



12.1 Formulas for Use before Hematoxylin Stains 



12.1 AUi 1944 13685, 95:317 



formula: water 25, 95% alcohol 65, tannic acid 10, acetic acid 10, phenol 5 



12.1 Anderson 1922 11977, 5:65 



formula: ads 12.1 Weigert 1896 90, 2% calcium hypochlorite 10 



12.1 Anderson 1942 11431, 54:258 



formula: water 100, potassium dichromate 3.75, chromium fluoride 1.875, phospho- 

 molybdic acid 0.64, calcium hypochlorite 0.2 



12.1 Bacsich 1937 11025,72:163 



formula: water 100, chromic acid 1, ferric chloride 1 



12.1 Benda 1893 22246, 7:161 



formula: water 40, sulfuric acid 15, ferrous sulfate 80, nitric acid 15 



preparation: Dissolve the sulfate in the sulfuric acid and water. Heat to 50°C. 



Add nitric acid. 

 note: This is roughly equivalent in iron content to a 70% solution of ferric sulfate. This 

 salt, however, is so hygroscopic that it is almost impossible to prepare a solution of 

 known concentration from the solid. The additional acids both stabilize the solution 

 and render it more effective as a mordant. For a solution sometimes known as Benda' s 

 Mordant see Chapter 18, F 4600.1010 Benda (1911). 



12.1 Chura 1925 23632, 42 :59 



formula: water 100, chromium fluoride 0.5, chrome alum 0.5, potassium dichromate 2.5 

 recommended for: use after F 5670.1000 Chura 1925 to mordant cytoplasmic inclu- 

 sions. 

 note: See also ADS 11.1 Chura 1925. 



12.1 Clara 1933 23639b, 17 :698 



formula: water 100, potassium dichromate 3, chrome alum 1.5, chromic acid 1.25, 

 ammonium molybdate 1.25 



12.1 Cole 1916 19938,44:452 



formula: 95% ale. 50, water 50, ferric chloride 5, acetic acid 10 



12.1 Drew 1920 11360,40:295 



formula: water 100, chromic acid 2, osmic acid 1 

 recommended for: tissue reviver before hematoxylin stains. 



12.1 Eichhorn 1941 1887a, 31:391 



formula: F 3700.0010 Zenker 1894 237.5, nitric acid 12.5 



