ADS 12.1-ADS 12.2 ACCESSORY DYE-STAINING SOLUTIONS 517 



note: Vastarini-Cresi 1915 (10157, 31:38) substitutes ammonium dichromatc for 

 potassium. 



12.1 Weigert 1891 test., 1910 ips. Weiyert's secondary mordant — compl. script. 



Ehrlich, Krause, et al. 1910, 1:231 

 formula: water 100, chroniiuin fluoride 2.5, acetic acid 2, copper acetate 5 

 preparation: Add fluoride to boiling water. Boil 5 minutes. Cool to 80°-90°C. and add 



other ingredients. 

 recommended for: use as a mordant before hemato.xylin staining, particularly of 



connective tissues in the central nervous system. See DS 21.212 and 21.22 (Chapter 



21). 

 note: This was named Gliaheizc by its inventor. Attempts to graft this word onto the 



English language appear, mercifully, to be failing. For an adaptation of this formula 



to fixative use see F 4600.1010 Benda (1911) (Chapter 18) and DS 21.22 Jakob 1913 



(Chapter 21). 



12.1 Williamson and Pearse 1923 see F 3670.0000 Williamson and Pearse 1923 



12.1 Wolters 1891 23632, 7:471 

 formula: water 100, vanadium chloride 2, aluminum acetate 6.5 



12.2 Formulas for Use before Other Stains 



12.2 Anderson 1923 11431,26:431 



formula: a. water 100, sodium sulfite 5, oxalic acid 2.5, potassium iodide 5, iodine 2.5, 



acetic acid b; B. water 95, ferric chloride 5 

 preparation of a: Dissolve the oxalic acid and sulfite in 95 water. Dissolve iodine and 



iodide in 5 water. Mix and add acetic acid. 

 recommended for: mordant before Victoria blue neuroglia stains. 

 note: The proportions of A and B vary according to the technique employed. Equal 



parts are generally satisfactory. 



12.2 Atkins 1920 11056, 5:321 



formula: water 100, sodium hydroxide 0.4, iodine 2 

 preparation: Dissolve the dry ingredients in 10 water. Dilute to 100. 



12.2 Bailey 1929 16913,27:11 



formula: water 100, ferric chloride 2.5, tannic acid 3.75 



12.2 BelUng 1921 651, 54:573 



formula: water 55, acetic acid 45, ferric oxide 5 

 recommended for: use as an ingredient of, or mordant before, aceto-carmine stains. 



12.2 Bethe 1896 see DS 21.21 Bethe 1896 



12.2 Burke 1922 see ADS 12.2 Lugol (1905) 



12.2 Casares-Gil test. Anselmier 16157b, 5 :33 



STOCK solution: 70% ale. 75, water 25, tannic acid 25, aluminum chloride 45, zinc 



chloride 25, rosaniline hydrochloride 3.57 

 working formula: stock 20, water 80 



recommended for: reviving old tissues and as a general mordant. 

 preparation : Dissolve the tannic acid and aluminum chloride in the ale. Dissolve the zinc 



chloride in the water and add drop by drop, with constant agitation, to first solution. 



Dissolve rosaniline hydrochloride in mixed solution. 



12.2 Cretin 1937 4285a, 14:163 



formula: water 100, aluminum chloride 0.7, ferric chloride 0.025, calcium chloride 5 

 PREPAR.vnoN: Dissolve each salt separately and mix in order given. 



12.2 David 1934 23684, 132 :240 



formula: water 90, potassium alum 5, mercuric chloride 1.25, tannic acid 4 



12.2 van Ermengen see AMS {not ADS) 21.1 van Ermengen 



