518 METHODS AND FORMULAS ADS 12.2 



12.2 Gordon 1939 11284,24:405 



formula: water 100, copper sulfate 12, mercuric chloride 2.5, potassium dichromate 1, 

 sodium sulfate 0.5 



12.2 Gough and Fulton 1929 11431,32:765 



formula: water 100, acetic acid 0.1, mercuric acetate 3 

 RECOMMENDED FOR: mordanting of fatty tissues prior to staining for mitochondria. 



12.2 Gram 1884 see AF 12.1 Gram 1884 



12.2 Gray 1926 11056, 12:273 



formula: sat. sol. (circ. 6%) potassium alum 50, 20% tannic acid 20, sat. sol {circ. 4%) 



mercuric chloride 20, sat. ale. sol. (circ. 5%) magenta 4 

 recommended for: use as Casares-Gil. 



note: This solution is unstable. It may, however, be prepared from the first three, and 

 the last ingredients, each as a separate solution. Liefson 1930 (11056, 20:203) sub- 

 stitutes 95% ale. for the sat. sol. mercuric chloride. 



12.2 Harris 1898 16185, N.V., 47 



formula: water 250, potassium ferricyanide to sat. {circ. 85 Gms.), osmic acid 0.1 

 recommended for: mordanting before DS 11.44 staining. 

 note: Harris recommends cooling to about O'C. before use. 



12.2 Kilduffe 1923 11006, 81:2182 



formula: water 100, iodine 0.3, potassium iodide 0.6, sodium bicarbonate 1. 



12.2 Liefson 1930 see ADS 12.2 Gray 1926 (note) 



12.2 Lugo! test. Lee 1905 see AF 12.1 Lugol (1905) 



12.2 Marquez 1933 3360, 112 :1056 



formula: water 100, chrome alum 10, potassium alum 10, potassium dichromate 10 

 recommended for: mordanting before staining for mitochondria. 



12.2 Michailow 1910 23632,27:19 



formula: water 250, sodium molybdate 20, 40% formaldehyde 1.5 

 recommended for: use before methylene blue nerve stains. See DS 21.211 (Chapter 21). 



12.2 Mullen and McCarter 1941 608b, 17 :289 



formula: water 95, acetic acid 5, chromic chloride 5. 



12.2 Muir see DS 23.217 Muir 



12.2 Oliver 1934 11250,55:266 



formula: water 100, tannic acid 6.5, ferrous sulfate 11, acid fuchsin 0.7 



12.2 Petragnani 1928 test. 1930 Ciferri 20540b, 5 :34 



FORMULA OF WORKING SOLUTION: Water 75, methanol 25, acetic acid 0.06 

 preparation: Stock I. In 100 0.1% acetic acid dissolve with heat 3 potassium alum 2 



and 0.5 lead acetate. Stock II. In 100 70% methanol dissolve 14 tannic acid and 4 



ferric chloride 

 WORKING solution: Mix 2 stock I with 1 stock II. Add 100 to 300 methanol. 



12.2 Ponselle 1913 6630,74:1072 



formula: abs. ale. 100, iodine 0.6 



12.2 Rossi test. 1904 Besson Besson 1904, 170 



formula: water 100, tannic acid 5, potassium carbonate 1 



12.2 Salazar 1923 763, 26 :60 



formula: water 70, acetic acid 30, tannin 1 



12.2 Semmens 1939 Microscope, 3:6 



formula: water 100, chromic acid 10, sodium uranate 0.5 

 use: Before crystal violet nuclear stains. 

 note: For the preparation of the sodium uranate see F 3800.0010 Semmens 1939b. 



