23 



Formulas and Techniques for Metal Stains 



Decimal Divisions Used in Chapter 



MS 00 GENERAL OBSERVATIONS 



01 Classification of methods and formulas 

 MS 10 OSMIC ACID 



11 Staining methods 



11.0 Typical example 



Demonstration of Golgi network in the ovary of the earthworm by the 

 Ludford 1925 method 



11.1 Staining solutions 



11.2 Neurological techniques 



11.21 Methods for degenerative changes 



11.22 Other neurological methods 



11.3 Histological methods 



11.4 Methods for cell inclusions 

 MS 20 GOLD 



21 Methods using gold alone 



21.0 Typical examples 



Demonstration of termination of the fourth cranial nerve in the 

 superior oblique muscle by the method of Ranvier 1889 



21.1 Staining solutions 



21.2 Techniques 



22 Methods using gold in combination with mercury 



22.0 Typical example 



Demonstration of protoplasmic neuroglia in the cerebral cortex by the 

 method of Cajal 1916 



22.1 Staining solutions 



22.2 Neurological methods 



22.21 Nerve cells and processes 



22.22 Neuroglia 



23 Methods using gold in other combinations 



23.0 Typical example 



Demonstration of the nervous elements in spinal cord by the method of 

 Gerlach 1872 



23.1 Staining solutions 



23.2 Neurological methods 



23.21 Nerve cells and processes 



23.3 Cytological methods 



23.4 Other methods 

 MS 30 SILVER 



31 Methods using sUver nitrate 

 31.0 Typical examples 



Demonstration of the nervous elements of the retina by the method of 

 Balbuena 1922 



Demonstration of neuoblasts and axons of the developing spinal cord 

 of the three-day chicken embryo by the method of Cajal 1910b 



522 



