528 METHODS AND FORMULAS MS 11.1-MS 11.21 



11.1 Mann 1894 see F 1300.0000 Mann 1894 



11.1 Orr 1900 see F 1000.0010 Orr 1900 



11.1 Rossolino and Busch 1896 tesl. 1897 Schiefferdecker 



23632, 14:55 

 formula: water 50, 95% ale. 50, 40% formaldehyde 0.2, osmic acid 0.2 



11.1 Swank and Davenport 1924 see F 1000.1010 Swank and Davenport 1924 



11.1 Swank and Davenport 1935 see F 1000.1010 Swank and Davenport 1935 



11.1 Takahashi 1908 see F 1600.0060 Takahashi 1908 



11.2 Neurological Techniques 

 11.21 methods for degenerative changes 



These methods are commonly known as Marchi methods. For further information see 

 Mettler 1932 (20540b, 7:95), Swank and Davenport 1934 and 1935 (20540b, 9:11 and ibid, 

 12:45). 



11.21 Anderson 1929 Anderson 1929, 68 



REAGENTS REQUIRED: A. 2% potassium iodate; B. F 1800.0000 Busch 1898; C. ADS 11.1 



Andeison 1929 

 method: [small pieces of formaldehyde-fixed tissue] — * A, 24 hrs. — > B, 7 daj's —* thor- 

 ough wash — > C, 7 days, 37°C. — » thorough wash -^ [celloidin sections] 



11.21 Busch 1898 see MS 11.21 Anderson 1929 for method, and F 1800.0000 Busch 1898 for 

 formula 



11.21 van Gehuchten test. 1927 Kingsbury and Johannsen 



Kingsburj' and Johannsen 1927, 8 :9 

 REAGENTS REQUIRED: A. 3.7% potassium dichromate; B F 1700.0000 van Gehuchten 



1927 

 method: [fresh tissue]-^ A, 3 wks. — > B, 3 wks. 2 or 3 changes —^ wash —^ [frozen 

 sections] 



11.21 Hamilton 1897 3464, 20:180 



REAGENTS REQUIRED: A. F 7000.0000 MiUler 1859; B. MS 11.1 Hamilton 1897; C. AMS 



21.1 Hamilton 1897; D. 0.25% potassium permanganate; E. 2% potassium sulfite 

 method: [whole brains]-^ A, till hardened, 3-5 months—* [celloidin section enclosed 

 between two coats of celloidin and stripped from the slide] — > B, 24 hrs. 37°C. — > 

 wash — » C, 24 hrs. 37 °C. -^ wash — > D, 24 hrs. — > wash — * E, till decolorized — * wash 

 -^ D ^> E cycle repeated twice — > wash — > C, fresh solution, 24 hrs., 37°C. — * wash — > 

 M 32.1 mountant 



11.21 Marchi 1896 19460, 12 :3 



reagents required: A. F 7000.0000 Miiller 1859; B. F 1700.0000 Marchi 1886 

 method: [1 cm. slices fresh tissue] -^ A, 3-5 days -^ wash — » B, 5-14 days — > wash -^ 

 [celloidin sections] 



11.21 Mettler 1932 20540b, 7:95 



reagents required: .4. 10% neutralized formaldehyde; B. 3% potassium dichromate; 



C. F 1700.0000 Marchi 1886 

 method: [pieces] — + A, 24 hrs. -^ [4 mm. slices] — > i5, 2 wks. — > rinse — * C, 5-14 days — > 



wash -^ [celloidin sections] 

 note: Sol. B should be "aged" 3 months. C should be replaced if it ceases to smell of 



osmic acid. 



11.21 Orr 1900 • 11431, 6:387 



reagents required: .4. F 7000.0000 Miiller 1859; B. Orr 1900 F 1000.0010 

 method: [A, several wks. to months] —> wash -^ [freehand sections]—* B, 1-2 days — > 

 balsam, via usual reagents 



