MS 11.21-MS 11.3 METAL STAINS 529 



11.21 Stewart 1936 11431,43:339 



REAGENTS Ri-xjiiREi): A. 2% pofassium dichromate; B. 1% osniic acid 



method: [30 m frozen sections of neutral formal dehyde-fixod material]— > wash — » A, 1 



day, 21°C. —> wash, till pale yellow —>■ B, in dark, 16-36 hrs. -^ wash —^ M 30 



mountant 

 note: Sections in whicli tlie normal myelin is too deeply stained may be differentiated 



by ADS 21.1 Pal 1887. 



11.21 Swank and Davenport 1935 20540b, 10:88 



REAGENTS REQUIRED: A. watcr 100, magnesium sulfate 5, potassium dichromate 3; 



B. 4% formaldehyde; C. Swank and Davenport 1935 F 1000.1010 

 method: [animal killed with nembutal] — + A, perfused through aorta — > [central nervous 



system removed] -^ B, 48 hrs. —* wash — > [celloidin sections] 



11.22 OTHEK NEUROLOGICAL METHODS 



11.22 Azoulay 1894 766, 10:25 



REAGENTS REQUIRED: .4. F 7000.1000 MuUer 1859; B. 0.002% osmic acid; C. 10% tannin 

 method: A, some months -^ [sections] —> 5, 5-15 mins. ^ rinse —> C, warmed till 



steaming — > wash — > balsam, via usual reagents 

 RECOMMENDED FOR: myelin sheaths. 



11.22 Bohm and Oppel 1907 Bohm and Oppel 1907, 261 



REAGENTS REQUIRED: A. 1% osmic acid; B. glycerol 

 method: [teased fibers of muscle on slide under coverslip] ^ A, 30 mins. in moist 



chamber -^ B, substituted for A without moving coverslip -^ [seal coverslip] 

 RECOMMENDED FOR: myelin sheaths. 



11.22 Champy, Coujard, and Coujard-Champy 1946 Acta Anatomica, 1 :233 

 REAGENTS required: A. water 100, osmic acid 0.25, sodium iodide 2.25 

 method: [2-3 mm. slices of fresh tissues] ^^ A, 24 hrs. -^ running water—* [paraffin 



sections] 

 RECOMMENDED FOR: Sympathetic nerve endings in gland cells. 



11.22 Heller-Robertson Irsl. 1929 Anderson Anderson 1929, 64 



REAGENTS REQUIRED: A. ADS 12.2 Weigert 1891; B. 1% osmic acid; C. 5% pyrogallic 



acid; D. ADS 21.1 Pal 1887, A and B sols. 

 method: [frozen sections] —* A, 24 hrs. — > wash -^ B, in dark, 30 mins. — > wash — > C, 



30 mins. —> wash — > D (sol. A), 30 sees. — > wash — > D (sol. B), till colorless — > balsam, 



via usual reagents 

 RECOMMENDED FOR: myelin sheaths. 



11.22 Rossolino and Busch 1896 test. Schiefferdecker 1897 



23632, 14:55 

 REAGENTS REQUIRED: A. 0.5% chromic acid; B. MS 11.1 Rossolino and Busch 1896 

 method: [sections of formaldehyde-fixed material] —> A, 2-3 hrs. —> rinse -^ .B, 24 



hrs. — > wash -^ balsam, via usual reagents 

 RECOMMENDED FOR: granule cells. 



11.3 Histological Methods 



11.3 Altmann 1878 see V31 Altmann 1878 



11.3 Cramer 1919 1200, 6:77 



REAGENTS REQUIRED: A. 2% osmic acid at 37°C. 

 method: Expose fragments to vapor from A in stoppered bottle for 13-^ hrs. at 37°C. -^ 



[sections via usual reagents] 

 RECOMMENDED FOR: Cell differentiation in adrenal gland. 



11.3 Hamann 1885 see F 1000.0010 Hamann 1885 



11.3 Hermann 1891 1780,37:4 



REAGENTS REQUIRED: A. F 1200.0010 Hermann 1889; B. 90% ale; C. crude pyroligneous 



acid 

 method: a, overnight —* wash B, 1 to 2 wks. — > C, 12-18 wks. — > wash 



