530 METHODS AND FORMULAS MS 11.3-MS 11.4 



11.3 Hirschler 1918 see F 1300.0000 Hirschler 1918 



11.3 Kolossow 1892 23632, 9:39 



REAGENTS REQUIRED: A. MS 11.1 Kolossow 1892; B. AMS 21.1 Kolossow 1892; C. 0.05% 



osmic acid 

 method: [small pieces or objects] — » A, 15 mins. -^ wash — > B, 5 mins. -^ C, wash -^ 



balsam, via usual reagents 

 note: Lee 1891 (23632, 9 :185) claimed this method on the grounds that he had in 1887 



(6011, 4:110) published a description of it. Kolossow 1892 (23632, 9:316) pointed out 



that Lee had given only a general indication of an uncontrolled method of reducing 



osmic acid with tannin. 

 recommended for: general histological staining. 



11.3 Krajian 1940 1887a, 30:766 



REAGENTS REQUIRED: A. 1% osmic acid; B. \% phloxine 

 method: [10 M frozen sections of formaldehyde-fixed material]—* A, 60°C., 5 mins.—* 



thorough wash-^ B, 1 min. — * wash -^ M 12.1 mountant 

 recommended for: fat in frozen sections. 



11.3 Lee 1887 6011,4:110 



reagents required: A. 1% osmic acid; B. 0.5% pyrogallol 

 method: a, 1-2 days ^ wash -^ B, till green-brown throughout-* [paraffin sections 



via usual reagents] 

 recommended for: general histological staining. 



11.3 Lee 1905 Lee 1905, 255 



reagents required: A. F 1200.0010 Hermann 1889 or F 1600.0010 Flemming 1882; 



B. 0.25% pyrogallol 

 method: a, not more than 30 mins. -* B, 24 hrs. -^ [sections by paraffin technique] 

 recommended for: general histological staining. 

 note: Lee 1905 {loc. cit.) states that this method has been attributed to von Maehren- 



thal. 



11.3 von Maehrenthal see MS 11.3 Lee 1905 (note) 



11.3 Sjovall 1905 764,30:261 



reagents required: A. 4% formaldehyde; B.2% osmic acid 



method: [small fragments]-* A, 8 hrs. to 2 days ^ wash-* B, 2 to 15 days -^ 3 n 

 paraffin sections, via usual techniques 



11.3 Woronin 1898 test. 1948 Romeis Romeis 1948, 304 

 reagents required: A. \% osmic acid; B. sat. aq. sol. tannin 



method: [sections] -^^ waters A, 10 mins.-* B, 10 mins.-* A, 20 mins.-* abs. ale, 



thorough wash -^ balsam, via xylene 

 recommended for: epithelial tissues. 



11.4 Methods for Cell Inclusions 



These techniques for the demonstration of the Golgi apparatus are commonly referred to 

 as Mann-Kopsch techniques. 



11.4 Gatenby 1920a 17510,64:267 



reagents required: A. 2% osmic acid at 37°C.; B. F 1700.0000 [AltmanMSOO or 



F 1670.0000 Champy 1913 

 method: expose fragments to vapor from A in stoppered bottle at 37 °C. for 112 hrs. — » 



A, several days — * or B, several days -* wash — * sections via usual reagents 



11.4 Gatenby 1920b 17510, 64:267; Langeron 1942, 646 



REAGENTS REQUIRED: A. F 1300.0000 Mann 1894; B. 2% osmic acid; C. turpentine 

 method: [small fragments] -* A, 15 mins. to 3 hrs. -^ wash -^ 5, 2 to 3 wks. in dark -^ 



3 M sections by paraffin technique — * C, till differentiated 

 NOTE : Langeron 1942, 646 refers to this as the Kopsch-Gatenby technique. 



