MS 11.4 METAL STAINS 531 



11.4 Hirschler 1918 1780, 89:271 



REAGENTS REQUIRED: A. F irjOO.OOO IlirschlcF 1918; B. 2% osinic acid 



method: [small fragments] — > A, 1-3 hrs. — > wash — > B, 12-16 days —» wash — » paraffin 



via chloroform 

 note: This was reprinted, with slight alterations in the timing, in 1924 (6630, 90:83). 



11.4 Kopsch 1902 20170, 40:929 



reagents required: A. 2% osmic acid 



method: [small fragments] —> rinse -^ A, in dark, 2 wks. — > wash — » [3 n paraffin 

 sections, via usual reagents] 



11.4 Kopsch-Gatenby test. Langeron 1942 see MS 11.4 Gatenby 1920b (note) 



11.4 Ludford 1925 113G0, 45:31 



reagents required: .4. F 1300.0000 Mann 1894; B. 2% osmic acid; C. turpentine 



method: [small pieces fresh tissue] —> A, ^^ to 1 hr. — » wash — > B, sufficient to cover 



piece on bottom of 1 oz. stoppered bottle, 10 days to 2 wks. — > water at 38°C., 1-2 



days -^ wash — » [paraffin sections by usual techniques] — > xylene, till wax removed -^ 



C, if differentiation necessary -^ balsam, via usual reagents 



11.4 "Mann-Kopsch" see note under 11.4 above. 



11.4 Nassanow 1923 1780,97:136 



reagents required: A. F 1670.0000 Nassanow 1923; B. 2% osmic acid at 35°C. 

 method: [small pieces fresh tissue]^ A, 24 hrs. — > wash—* B, 3 to 7 days, 35°C. — > 

 wash -^ [paraffin sections by usual techniques] 



11.4 Newcomer 1940 20540b, 15:89 



reagents required: A. F 4700.0000 Zirkle 1934; B. 2% osmic acid; C. 1% potassium 



permanganate; D. 3% oxalic acid 

 method: [growing root tips] -^ A, 4S hrs. -^ wash, overnight, — > B, changed alternate 

 days, 4-6 days -^ wash, overnight — > [5 m paraffin sections] — * water -^ C, 5 mins. — > 

 rinse — > D, 2-3 mins. — > thorough wash — > balsam, via usual reagents 

 recommended for: mitochondria in plant cells. 



11.4 Weigert test. McClung 1929, 208 see DS 11.4 Weigl 1912 note 



11.4 Weigl 1912 Mann-Kopsch — compl. script. 4346,23:1 



reagents required: A. F 1300.0000 Mann 1894; B. 2% osmic acid 



method: [small fragments] —* A, 30 mins. to 2 hrs. -^ wash —* B, 10 days to 3 wks. — » 



wash -^ [sections via usual techniques] 

 note : This is almost universally referred to as the Mann-Kopsch technique for the reason 



that the former invented solution A for another purpose, while the latter recommended 



solution B for the present one. McClung 1929, 208 refers to the method as WeigerVs 



Mann-Kopsch. 



]VIS 20 Gold 



Gold is used eitlaer by direct application witli osmium or other metals. Techniques 

 of tlie chloride to partially hydrolized tis- in which gold is used to replace silver- 

 sues, or in combination with mercury, impregnated structures (the toning reac- 

 either from complex solutions or by sue- tion of the photographer) are given under 

 cessive applications, or in combination silver staining in MS 30 below. 



MS 21 METHODS USING GOLD ALONE 



These are the most primitive of all the endings in muscle as the classic gold- 

 metal-staining techniques though, when lemon-juice method of Ranvier given be- 

 they are successful, they yield beautiful low. The effect is presumably produced by 

 results. There is still no method as satis- the reducing action exercised by the sheath 

 factory for the demonstration of nerve of the nerve on gold chloride, but there is 



