534 METHODS AND FORMULAS MS 21.1-MS 21.2 



MS 21,1 Staining Solutions 



21.1 Jabonero 1935 test. 1936 Findlay 11360, 56:160 



formula: water 100, glucose 1, gold chloride 0.02 



21.1 Kolossow 1888 23632, 5 :52 



formula: water 245, hydrochloric acid 25, gold chloride 25 



21.1 Ranvier 1880 17510, 80:456 



formula: water 200, formic acid 50, gold chloride 2 



21.1 Stoehr 1894 test. 1907 Bohm and Oppel Bohm and Oppel 1907, 258 

 formula: water 200, formic acid 50, gold chloride 2 



preparation: Dissolve the chloride in the dilute acid. Bring to boil. Cool. Repeat 3 

 times. Filter. 



MS 21.2 Techniques 



Unless specific recommendations are made, it is to be understood that all the following 

 methods are intended for nerve endings in muscle. 



21.2 Apathy 1897 14246, 12:718 

 reagents required: A. 1% gold chloride; B. \% formic acid 



method: [fresh tissue] — > A, in dark — * blot — > B, 6 to 8 hrs., evenly illuminated from 

 all sides 



21.2 Beckwith test. circ. 1938 Wellings Wellings circ. 1938, 130 



reagents required: A. 1% gold chloride; B. 20% sodium hydroxide; C. 10% potas- 

 sium carbonate; D. 10% potassium iodide 

 method: [sections from F 4700.0000 Erlitzky 1877 or F 7000.0000 Muller 1859 fixed 

 material] — > water -^ A, 5-6 hrs. -^ rinse -^ B, 3 mins. -^ drain — > C, 30 mins. — > 

 drain — > D, till differentiated 

 recommended for: nerves in teeth. 



21.2 Bensley and Bensley 1936 test. 1952 ips. Cowdry 1952, 8 



reagents required: .4. 10% gold chloride; B. 4%, neutralized formaldehyde; C. 1% 



toluidine blue; D. water 100, ammonium molybdate 2.5, potassium ferrocyanide 0.5 

 method: [inject mouse intravenously through tail with 1 ml. A] — >• [after death fix lungs 



in B] —>■ [paraffin sections on slide] -^ water — » C, 10 mins. — >• rinse -^ D, 5 mins. — > 



balsam, via usual reagents 

 recommended for: alveolar epithelium of lung. 



21.2 Boccardi 1886 test. Lee 1905 see MS 21.2 Manfredi 1881 (note) 



21.2 Bohm 1907 test. 1907 Bohm and Oppel Bohm and Oppel 1907, 437 



reagents required: A. 50%, formic acid; B. 1% gold chloride; C. AMS 21.1 Pritchard 



1907 

 method: [small fragments, fresh tissue] — ^ A, 20 mins. —>■ rinse —>■ B, 20 mins. -^ rinse 



—>■ C, large volumes, 24-36 hrs. in dark — > paraffin sections via usual reagents 

 note: This technique is .sometimes referred to Carriere 1882 (1780, 21:146) who, how- 

 ever, attributes it without reference to Bohm as do, still without reference, most other 

 authors including those cited. 



21.2 Carriere 1882 see MS 21.2 Bohm 1907 (note) 



21.2 Cole 1946 20540b, 21 :23 



reagents required: A. 10% citric acid in 0.9% sodium chloride; B. 1% gold chloride; 



C. 20% formic acid 

 method: [muscle pieces] —► .4, 10 mins. — > Zi. 1 hr. -^ C. 10-20 hrs. -> glycerol by 

 evaporation from glycerol-alcohol mixtures 



21.2 Cornheim 1867 see MS 21.2 Manfredi 1881 (note) 



