MS 21.2 METAL STAINS 535 



21.2 Dependorf 1913 7282, 31 :377 



REAGENTS REQUIRED: A. 30% foriiiic acid ; B. 1% gold chloride; C. formic acid 

 method: [small pieces of fresh teeth] — > A, 5-10 mins. -^ B, 2-6 hrs., in dark — > A, 1 



day, in dark -^ C, 1 day, in dark -^ [celloidin sections] 

 recommended for: nerves in teeth. 



21.2 Drasch 1887 23632, 4:492 



REAGENTS REQUIRED: A. 0.5% gold chloride; B. 20% formic acid 

 method: [ pieces of fresh tissue, left without preservative at 4°C. for 48 hrs.] — > A, in 



dark, >2 to ^i hrs. — > rinse -^ B, till nerves visible -^ glycerol, several changes 

 recommended for: nerve endings in alimentary canal. 



21.2 Flechsig 1884 see MS 21.2 Ranvier 1880 (note) 



21.2 Freud teat. 1896 Kahlden and Laurent Kahlden and Laurent 1890, 159 



reagents required: A. 0.5% gold chloride in 50% ale; B. 5% sodium hydroxide; C. 



10% potassium iodide 

 method: [small pieces, or sections, of dichromate-fixed material] -^ water -^ A, 3-5 hrs. 



— > wash -^ B, 2-3 mins. — > C, 5-15 mins. —> wash -^ balsam, via usual reagents 

 recommended for: axis cylinders. 



21.2 Graven 1925 3464, 48 :380 



reagents required: A. 25% formic acid; B. 1% gold chloride 



method: [fresh tissue] —> A, 10-15 mins. -^ blot -^ B, 20 mins., in shade -^ blot -^ A, 

 10-15 mins, -^ B, 24 hrs., in dark -^ blot -^ A, 10-15 mins. -^ blot -^ B, 24 hrs., in 

 dark -^ wash -^ glycerol 



21.2 Hanazawa 1917 7141,59:125 



reagents required: A. 1% gold chloride in 95% ale; B. 5% potassium hydroxide; 



C. 10% potassium iodide 



method: [embed tooth in resin, grind section to half millimeter (see Chapter 10)] —> A, 

 2-3 days, in dark -y wash -> B, 15-20 mins. -^ wash —>■ C, 12-24 hrs. -* [grind thin] 

 — > balsam, via usual reagents 



recommended for: dentine. 



21.2 Henocque test. 1895 Rawitz Rawitz 1895, 81 



reagents required: A. 1% gold chloride; B. sat. sol. tartaric acid 

 method: [small pieces] — > A, 15 mins. -^ rinse — > B, till reduced 



21.2 Kolossow 1888 23032, 5 :52 



reagents required: A. MS 21.1 Kolossow 1888; B. 0.02% chromic acid 

 method: [fresh tissue] -^ A,S hrs. — > blot -^ JB, 2 or 3 days in dark 



21.2 Jabonero 1935 test. 1936 Findlay 11360, 56:160 



reagents required: A. 50% glucose; B. MS 21.1 Jabonero 1935; C. 5% sodium car- 

 bonate 

 method: [frozen sections of fresh tissue] -♦ A, 10 mins., 60°C. -^ B, warmed till sections 



violet — > balsam, via usual reagents 

 recommended for: myelin sheaths. 



21.2 Lowit 1875 20170,71:1 



reagents required: A. 50% formic acid; B. 1% gold chloride; C. 25% formic acid; 



D. formic acid 



method: [fresh skin] -^ A, until epidermis peels off -^ B, 15 mins. —> C, in dark 24 hrs. 

 — > D, in dark 24 hrs. — > [sections by paraffin technique] — * balsam, via usual reagents 

 recommended for: nerve endings in skin. 



21.2 Manfredil881 1946,5:30 



REAGENTS REQUIRED: A. 1% gold chlondc; B. 0.5% oxalic acid 



method: [fresh tissue] -^ A, 30 mins. — » rinse -^ B, until reduced -^ glycerol mounts 

 note: This method is essentially that of Cornheim 1867 (22575, 34:606) save that 

 oxalic acid has been substituted for the acetic acid of the original. Boccardi 1886 

 (test. Lee 1905, 251) substituted MS 23.1 Boccardi 1886 for B above. 



