MS 31.1-MS31.21 METAL STAINS 551 



31.1 del Rio-Hortega 1921 3232, 21:1 



formula: water 100, silver nitrate 2, pyridine 1, 95% ale. 5 



31.1 del Rio-Hortega 1932 lesl. 1933 Cajal and de Castro 



Cajal and de Castro 1933, 262 

 formula: water 100, 95% ale. 1.5, silver nitrate 2, pyridine 1.5 



31.1 Shanklin 1951 Cowdry 1952, 270 



formula: water 100, silver nitrate 10, pyridine 1 



31.2 Neurological Methods 



31.21 nerve cells and processes 



31.21 Ascoli 1911 3381,25:177 



REAGENTS REQUIRED: A. 5% silvcr uitrate in 95% ale; B. 10% silver nitrate; C. AMS 



21.1 Ascoli 1911; D. glycerol; E. AMS 22.1 Cajal 1910 

 method: [leeches, slit open and tied, not pinned, round a cork] — > A, till hard, few mins. 



— * A, fresh solution after removal from cork, 24-48 hrs. at 40°C. — ^ B, 24-48 hrs. 



-> rinse -^ C, 5-8 hrs. -^ wash -^ D -^ teased preparations -^ E, 5 mins. —> M 11.1 



Apathy 1892 

 RECOMMENDED FOR: lecches. 



31.21 Balbuena 1922 21344, 20:31 



REAGENTS REQUIRED: .4. AMS 12.1 Cajal 1910a; B. AMS 12.1 Balbuena 1922; C. MS 

 31.1 Balbuena 1922; D. AMS 21.1 Balbuena 1922; E. AMS 22.1 Balbuena 1922; F. 5% 

 sodium thiosulfate 



method: [small pieces] -^^ .4, 12-48 hrs. -^ [sections by celloidin technique] ^ B, 2 to 

 20 days —>■ rinse, 70% ale. -^ drain —>■ 20 ml. C. heated to steaming, 3-10 mins. -^ 

 cool — > add to sections in 20 ml. C, 2 or 3 drops D {A sol.) — + shake gently —> add 2 or 

 3 drops D {B sol.) — > shake gently, leave 10-15 mins. —>■ wash —^E,5 mins. —> F, 2 

 mins. — > balsam, via usual reagents 



RECOMMENDED FOR: retina. 



note: a detailed description of this technique is given under MS 31.0 above. 



31.21 Bartelmez 1915 11135, 25:87 



reagents required: .4. F 0000.0010 Bartelmez 1915; or F 0000.0010 Bartelmez 1915; 



B. 1.0%, silver nitrate; C. 1.5% silver nitrate; D. 2% silver nitrate; E. AMS 21.1 



Cajal 1910 

 method: [fish larvae] —> A, 60-90 mins. — > 80% ale, wash —>■ wash —>■ B, 24 hrs. 35°C. 



-^ C, 24 hrs. 35°C. — >• [repeat B -^ C —^ D cycle, till material becomes brown] —> 



rinse — + E, 24 hrs. — » wash -^ [sections by paraffin technique] 

 recommended for: fish larvae. 



31.21 Blair and Davies 1935 590, 69 :303 



reagents required: .4. water 81, 40% formaldehyde 9, ammonia 5; B. pyridine; C. 2% 



silver nitrate; D. DS 21.1 Noguchi 1913 

 method: [pieces fixed in neutralized formaldehyde] —> A, 2 days -^ wash, 2 days—* 



B, 2 days —> thorough wash, distilled water — + C, 4 days, 35°C. — > thorough wash — » 



D, 24 hrs. — > wash — > [sections] 

 recommended for: nerves in heart. 



31.21 Boule 1908 15063, 10:15 



reagents required: A. F 0000.1010 Boule 1908 or AMS 12.1 Boule 1908; B. 3% silver 



nitrate in 15% alcohol; C. Boule 1908 AMS 21.1 

 method: [small pieces] —>■ A, 24-48 hrs. — > drawn or blot — > i5, 7 days, 35°C. -^ rinse, 

 15% alcohol — > C, 24 hrs. — > [section by paraffin technique] 



31.21 Buxton test. 1930 Eltringham Eltringham 1930, 91 



REAGENTS REQUIRED: .4.1% silvcr nitrate; B. 1.5% silver nitrate; C 1% gold chloride; 



D. 2% pyrogallol 

 method: [fresh material] —> .4, 10 days, in dark — » [paraffin sections] — > water — > B, 10 



mins., bright sunlight — » wash — > C, 2 mins. — > Z>, 5 mins. — » wash — » balsam, via 



usual reagents 

 recommended for: insect brains. 



