552 METHODS AND FORMULAS MS 31.21 



31.21 Cajal 1910a methods 1, lA, IB, iC—auct. 21344, 8:3 



REAGENTS REQUIRED: A. 1.5% silver nitrate (or see below); B. AMS 21.1 Cajal 1910; 

 C. AMS 22.1 Cajal 1910 



METHOD 1 : [fresh tissue] -^ A, 3-4 days, 35°C. -^ rinse -* B, 24 hrs. -* [section by paraffin 

 technique, bring sections to water] -* C (optional), till faint stains sufficiently dark- 

 ened — * balsam via usual reagents 



RECOMMENDED FOR! nerve cells and processes in embryos in lower mammals. 



Method lA: Substitute 5% silver nitrate for A above. 



RECOMMENDED FOR: scnsory nerve endings, invertebrates. 



Method IB: Substitute 0.75% silver nitrate for A above. 



RECOMMENDED FOR: Very young embryos. 



Method IC: Substitute MS 31.1 Cajal 1910 for A above. 



RECOMMENDED FOR: human tissues and embryos. 



31.21 Cajal 1910b methods 2, 2 A, 2B, 2C, 2D—auct. 21344, 8:7 



REAGENTS REQUIRED: A. 95% alcohol (or see below); B. 1.5% silvernitrate; C. AMS 21.1 



Cajal 1910; D. AMS 22.1 Cajal 1910 

 METHOD 2: [fresh tissue] — » A, 24 hrs. -^ drain or blot -^ B, 4-5 days, 35°C. -^ rinse — > 



C, 24 hrs. — > [section by paraffin technique, bring sections to water] — > D (optional), 



till faint stains darkened — * balsam, via usual reagents 

 RECOMMENDED FOR: general use. 



Method 2A: Substitute AMS 12.1 Cajal 1910d, for A above. 

 RECOMMENDED FOR: general use when method #2 is unsatisfactory. 

 Method 2B: Substitute AMS 12.1 Cajal 1910a, for A above. 

 RECOMMENDED FOR: peripheral nerve endings. 

 Method 2C: Substitute AMS 12.1 Cajal 1910e, for A above. 

 RECOMMENDED FOR: Spinal cord. 

 Method 2D : Substitute AMS 12.1 Cajal 1910, for A above, and precede A by 24 hrs. in 



allyl alcohol. 

 RECOMMENDED FOR: human cerebrum and cerebellum. 



31.21 Cajal 1910c methods 3, SA, 3B—auct. 21344, 8:9 



REAGENTS REQUIRED: A. AMS 12.1 Cajal 1910 (or see below); B. 1.5% sUver nitrate; 

 C. AMS 31.1 Cajal 1910; D. MS 22.1 Cajal 1910 



METHOD 3 : [fresh tissue] -^ A, 20 to 48 hrs. -^ drain or blot — >• 5, 4 days at 40°C. or until 

 light gray -^ rinse -^ C, 24 hrs. — > [sections by paraffin technique, bring sections to 

 water] -^ D (optional) till faint stains darkened — > balsam, via usual reagents 



RECOMMENDED FOR: neurofibrils of large cells. 



Method 3A: Substitute AMS 12.1 Cajal 1910f for A above. 



RECOMMENDED FOR: buds of Auerbach. 



Method 3B: Substitute AMS 12.1 Cajal 1910g for A above. 



RECOMMENDED FOR: buds of Held. 



31.21 Cajal 1910d methods 4, 4A—auct. 21344, 8:11 



REAGENTS REQUIRED: A. 4% ueutral formaldehyde; B. AMS 12.1 Cajal 1910; C. 1.5% 



silver nitrate; D. AMS 21.1 Cajal 1910; E. AMS 22.1 Cajal 1910 

 METHOD 4: [fresh tissue] — > A, 6-8 hrs. — > running water, 6 hrs. — > B, 24 hrs. — > drain or 



blot — > C, 4 days at 40°C. — > rinse -^ D, 24 hrs. — > [section by paraffin technique, 



bring sections to water] — > E (optional), till faint stains darkened -^ balsam, via 



usual reagents 

 RECOMMENDED FOR: arborizations in adult cerebellum. 

 Method 4A: Substitute fixative of class F 0000.1000 for A above. 

 RECOMMENDED FOR: neurofibrils of large cells. 



31.21 Cajall910e method 5— auct. 21344,8:12 



REAGENTS REQUIRED: A. 50% pyridine; B. pyridine; C. 90% alcohol; D. 1.5% silver 



nitrate; E. AMS 21.1 Cajal 1910; F. AMS 22.1 Cajal 1910 

 METHOD 5: [fresh tissue] — > A, 6 to 8 hrs. — > B, 18-24 hrs. — > running water 6 hrs. -^ C, 



24 hrs. —>■ drain or blot -> D, 4-5 days, 35°C. — » rinse — ♦ D, 24 hrs. -^ [section by 



