MS 31.21 METAL STAINS 553 



paraffin technique, bring sections to water] — » F (optional), till faint stains darkened 

 — ♦ balsam, via usual reagents 

 RECOMMENDED FOR: neurogenesis and regenerative processes. 



31.21 Cajal 1910f methods 6, GA—aud. 21344, 8:14 



REAGENTS REQUIRED: A. 10% chloral hydrate) B. AMS 12.1 Cajal 1910 (or see below); 



C. 1.5% silver nitrate; D. AMS 21.1 Cajal 1910; E. AMS 22.1 Cajal 1910 

 METHOD 6: [fresh tissue] -^ A, 24 hrs. — > rinse — > B, 24 hrs. -* drain or blot -^ C, 4-5 



days, 40°C. -^ rinse -^ D, 24 hrs. — > [section by paraffin technique, bring sections to 



water] — » E (optional), till faint stains darkened 

 RECOMMENDED FOR: PurkinJG cells, motor end plates. 

 Method 6A: Omit B from above. 



31.21 Cajall921 21344,19:71 



REAGENTS REQUIRED: A. AMS 31.1 Cajal 1921; 5. 95% alcohol; C. AMS 21.1 Cajal 1921; 



D. AMS 22.1 Cajal 1921 



method: [sections of formaldehyde material by freezing technique] -^ wash -^ A, 12-48 

 hrs. or till light brown -* B (optional), 30 sees. -^ blot or drain -^ C, 24 hrs. -^ D, till 

 axons of meduUated fibers — > balsam, via usual reagents 



RECOMMENDED FOR: axons of meduUated fibers. 



31.21 Cajal 1925a 21344,23:237 



REAGENTS REQUIRED: A. MS 31.1 Cajal 1925; B. AMS 21.1 Cajal 1925; C. 0.2% gold 



chloride; D. 5% sodium thiosulfate. 

 method: [30-40 m sections, by freezing technique, of formaldehyde material] -> A, few 

 mins. or till hght brown —> rinse, 95% alcohol-* B, 5-15 mins. -* wash — > C (op- 

 tional), tiU required shade -+ D, 5 mins. -^ wash — * balsam, via usual reagents 



RECOMMENDED FOR: aXOnS. 



note: This method is frequently confused in the literature with MS 33.21 Cajal 1925. 



31.21 Cajal 1925b test. 1933 ips. Cajal and de Castro 1933, 362 



REAGENTS REQUIRED: A. AMS 12.1 Cajal 1925; B. 2% silver nitrate; C. AMS 21.1 Cajal 



1925; D. 0.2% gold chloride; E. 5% sodium thiosulfate 

 method: [frozen sections of formaldehyde-fixed material] — > A, 1-2 hrs. -^ quick wash 



-> B, 1-5 mins., 45°-50°C. —> 95% ale, quick rinse -^ C, 1-3 mins. -^ wash -* D, till 



gray —* E, 5 mins. -^ balsam, via usual reagents 

 recommended for: nerve endings in tongue muscle. 



31.21 Cajal 1929 21344, 26:1 



REAGENTS REQUIRED: A. AMS 11.1 Cajal 1929; B. AMS 12.1 Cajal 1929; C. MS 31.1 



Cajal 1929; D. AMS 21.1 Cajal 1929; E. any AMS 22.1 sol. 

 method: [fragments of fresh tissue] -^ A, 1-2 days — > frozen sections -^ B, till required 



-^ C, 10 hrs. ^95% ale, 24 hrs. -^ D, 12 hrs. -♦ wash -* E, 10 mins. -^ balsam, via 



usual reagents 

 RECOMMENDED FOR: sections of cerebellum. 



31.21 Cajal 1927 test. 1933 ips. Cajal and de Castro 1933, 188 



REAGENTS REQUIRED: A. AMS 12.1 Cajal 1927; B. 1.5% silver nitrate; C. AMS 21.1 



Cajal 1910 

 method: [fragments of fresh tissue] -^ A, 24-48 hrs. -♦ wash, overnight^ B, 3 days, 



40°C. — > rinse — >• C, 24 hrs. —* [sections] 

 RECOMMENDED FOR: nerve fibers. 



31.21 Cajal 1930 test. 1933 ips. Cajal and de Castro 1933, 190 



REAGENTS REQUIRED: A. 5% neutralized formaldehyde; B. 70% pyridine; C. 1.5% silver 



nitrate; D. AMS 21.1 Cajal 1910 

 method: [fragments of fresh tissue]-* A, 2-3 days—* wash-* B, 1-2 days-* running 



water, 24 hrs. -* C, 3 days, 37°C. -* wash -^ D, 24 hrs. -^ celloidin sections 

 recommended for: brains of small mammals. 



