554 METHODS AND FORMULAS MS 31.21 



31.21 de Castro 1926 21344, 23:427 



REAGENTS REQUIRED: A. AF 21.1 (le Castro 1926; B. 0.3% ammonia in 95% alcohol; 



C. 15% silver nitrate; D. AMS 21.1 Cajal 1910; E. 0.2% gold cliloride; F. 5% sodium 

 thiosulfate 



method: [fresh material] — * A, till decalcified -^ wash, 24 hrs. — » slice, 1 mm. thick -^ 

 B, thorough wash — > wash — > C, 5-7 days, 37°-40°C. -^ wash — > D, 3-4 hrs. — > wash 

 — * [sections] -^ E, 1-5 mins. — > F, 5 mins. — » wash — > balsam, via usual reagents 



RECOMMENDED FOR: nervcs in teeth. 



31.21 Cher 1933 1879, 29:344 



REAGENTS required: A. \% ammonia in 95% ale; B. pyridine; C. 2% silver nitrate; 



D. AMS 21.1 Chor 1933 



method: [fresh muscle] -^ A, 24 hrs. -^ wash -^ B, 48 hrs. — > thorough wash — > C, in 

 dark, 72 hrs. — > rinse—* D, 6-8 hrs. ^ rinse —> 95% ale.,-* [paraffin sections by 

 usual techniques] 



recommended for: motor end plates in primate biceps. 



31.21 Cowdry 1912 10157, 29:1 



reagents required: A. 1.5% silver nitrate; B. AMS 21.1 Cowdry 1912; C. 0.1% gold 



chloride; D. 5% sodium thiosulfate 

 method: [pieces fixed in F 0000.0010 Carnoy 1887] -^ water -^ A, 3 days at 40°C. -* 



rinse — * B, 24 hrs., in dark -^ [paraffin sections] — > water — » C, 2 hrs. — > rinse -^ D, 



5 mins. -^ balsam, via usual reagents 

 recommended for: nerve fibrils. 



31.21 Davenport 1930 1879,24:690 



REAGENTS REQUIRED: A. MS 31.1 Daveuport 1930; B. AMS 21.1 Davenport 1930; C. 



0.01% gold chloride; D. 5% sodium thiosulfate 

 method: [celloidin sections mounted on slides and varnished with 2% celloidin] —+80% 



ale, 5 mins. —* A, overnight or till yellow -^ ale, rinse — > 5, 2 mins. — > ale, wash — > 



C (optional), till desired color — + D (optional), 2 mins. -^ ether-alcohol to remove 



celloidin — > balsam, via usual reagents 

 RECOMMENDED FOR: neurofibrils. 



31.21 Davenport, Windle, and Beech 1924 20540b, 9:5 



REAGENTS REQUIRED: A. 2% ammonia in 95% alcohol; B. 5% pyridin; C. 1.5% silver 



nitrate; Z). 4% pyrogallic acid 

 method: [fresh embryos]-^ A, 2 days — > B, 24 hrs. — > wash, 1-3 hrs. — > C, 2-3 days, 



37''C. — > wash 20 mins. (12 mm. embryos) to 1 hr. (20 mm. embryos) —* D, 4 hrs. —* 



wash —> section 

 RECOMMENDED FOR: embryos. 



31.21 Dogiel test. 1933 Cajal and de Castro Cajal and de Castro 1933, 357 



REAGENTS REQUIRED: .4. 1.5% formic acid in 90% ale; B. 2.5% silver nitrate; C. AMS 



21.1 Cajal 1910 

 method: [pieces of fresh tissue] —*• A, 1-2 days—* B, rinse —> B, fresh sol., 5-6 days, 



36°C. -* wash -* C, 24 hrs. -^ [sections] 

 RECOMMENDED FOR: corpuscles of Graudry and Herbst. 



31.21 da Fano 1920 11360, 40:157 



REAGENTS REQUIRED: A. AMS 11.1 da Fano 1920; B. 1.5% silver nitrate; C. AMS 21.1 



Cajal 1914; D. 0.2% gold chloride; E. 5% sodium thiosulfate 

 method: [small fragments fresh tissue] — > A, 6-8 hrs. — > rinse — > B, 24-48 hrs. — * rinse 



— > C, 24-48 hrs. -^ wash — > [section by paraffin technique, bring section to water] — * 



D, 2 hrs. -^ E, 5 mins. -* balsam, via usual reagents 



31.21 Favorsky 1930 766,70:376 



REAGENTS REQUIRED: A. 10% ammonia in 95% alcohol; B. pyridine; C. 2% silver 



nitrate; D. AMS 21.1 Cajal 1910a 

 method: [pieces from F 0000.0010 Favorsky 1930]-^ 50% ale, wash-* A, 2 days -^ 



wash -^ B, 1-2 days -^ wash — > C, 4-10 days, 37°C. -^ rinse -^ D, 24 hrs. -* wash -^ 



[paraffin sections] 



