MS 31.21 METAL STAINS 555 



31.21 Foley 1938 20540b, 13 :5 



REAGENTS REQUIRED: A. pyridine; B. 1% ammonia in 80% alcohol; C. 40% silver 

 nitrate; D. MS 33.1 Davenport 1930; E. AMS 21.1 Davenport 1930; F. 0.2% gold 

 chloride; G. 5% sodium thiosulfate 

 method: [sections of material from F 5000.1010 Boiiin 1897 or F 5000.1080 Foley 1938] 

 —> A, I hr. -^ abs. ale, rinse -^ [varnish on slide with celloidin] — > B, 12-24 hrs. -^ 

 80% ale, rinse -^ C, 6-8 hrs. 37°C. -> D, 16-24 hrs. -^ 95% ale, rinses E, till fine 

 axons chocolate brown — » 95% ale, rinse —* tap water, wash -^ F, 10 mins. — > wash 

 — > G, 3-5 mins. -^ wash —^ dammar, via alcohol and acetone 

 RECOMMENDED FOR: axons in picric fixed material. 



31.21 Foley 1939 763, 73:465 



REAGENTS REQUIRED: A. AMS 12.1 Foley 1939; B. a graded series of 50%, 40%, 30%,, 

 20%, and 10% alcohol each containing 15% pyridine; C. a graded series of 10%, 20%, 

 30%, 40%, 50%, 60%, 70%, 80%, and 90% aqueous pyridine; D. pyridine; E. 10% 

 silver nitrate; F. AMS 21.1 Ranson 1914 



method: [stretched nerves] -^ A, 4°C., 24 hrs. -^ B, 30 mins. in each -^ C, 30 mins. in 

 each (in 50% pyridine remove nerve from stretcher and thread through block of 

 cerebral cortex preserved in 50% pyridine) —^ D, 24 hrs. — > C, in reverse order, 30 

 mins. in each -^ water, thorough wash — > E, 3-5 days, 37.5°C. —y rinse —* F,2 days — » 

 [paraffin sections] 



31.21 Gooding and Stewart 1937 11977, 7:596 



REAGENTS required: A. 0.3% ammonia in 90% alcohol; B. 5% nitric acid; C.2% silver 



nitrate; D. AMS 21.1 Gooding and Stewart 1937 

 method: [pieces of teeth] — ^ A, 48 hrs. -^ rinse -^ B, 48 hrs. -^ running water, 24 hrs. —>■ 



C, 4-6 days, 37°C. — » rinse -^ D, 1-2 days, 37°C. -^ wash —> [paraffin or frozen 

 sections] 



RECOMMENDED FOR: nerves in pulp of teeth. 



31.21 Gurdjian 1927 1135,43:1 



REAGENTS REQUIRED: A. 1% ammonia in 95% ale; B. AMS 11.2 Cajal 1910d; C. 



0.75% silver nitrate; D. AMS 21.1 Ranson 1914 

 method: [whole brain stems] -^ A, 10-20 days, changing daily -^ drain and blot — > B, 



3-5 days, changing daily — > rinse -^ C, 2-4 wks., changing twice weekly —> rinse —> 



D, 7-10 days — > [sections by paraffin technique] 



31.21 Huber and Guild 1913 763, 7:253 



REAGENTS REQUIRED: A. 1% ammonia in 95% ale; B.7% nitric acid; C. pyridine; D.2% 

 silver nitrate; E. AMS 21.1 Ranson 1914 



method: [entire head, taken from animals injected via the heart with A]—* A, 2-4 days 

 — > wash -^ B, till decalcified — » wash -^^ ^, 3 to 8 days -^ rinse -^ C, 24 hrs. -^ D, 3 

 to 5 days, 35°C. in dark — > rinse — > E, 24 to 48 hrs. -^ [paraffin sections] 



31.21 Jahnel 1917 14370, 42:17 



REAGENTS REQUIRED: A. 10% formaldehyde; B. 1% uranium nitrate; C. 95% alcohol; 



D. 0.5% silver nitrate; E. AMS 21.1 Ranson 1914 



method: [fresh tissue] —> A, 15 days or [old formaldehyde-fixed material]—* B, 1 hr., 

 37°C. — > wash — > C, 1 wk. — > rinse — > I), 1 wk., 37°C., in dark — » wash, in dark — » 



E, 1-2 days, in dark -^ wash — > [sections by paraffin technique] 



31.21 Liesegang 1911 11848, 3:1 



reagents required: A. 1% silver nitrate; B. AMS 21.1 Liesegang 1911 



method: [sections of formaldehyde-fixed material by freezing technique] — * A, heated if 



necessary, till yellow —> pour off A, leaving thin layer, add B in excess-* wash — > 



balsam, via usual reagents 



31.21 McManus 1943 11431,65:503 



reagents required: A. MS 31.1 McManus 1943; B. AMS 21.1 Bodian 1936; C. 1% 



gold chloride; D. 2% oxalic acid; E. 5% sodium thiosulfate 

 method: [4-6 n paraffin sections] — ♦ water -^ A, 30 mins., 60°C. — > wash — > B, 10 mins. 



-^ wash — * C, 10 mins. — > rinse — > D, till fibers distinct — > wash — > E, 5-10 mins. -♦ 



wash -^ balsam, via usual reagents 

 recommended for: nerve fibers in spinal cord. 



