556 METHODS AND FORMULAS MS 31.21 



31.21 Miller 1944 test. 1944 Randall 20540b, 19:122 



REAGENTS REQUIRED: A. 20% silver nitrate; B. MS 33.1 Gros-Schultze (1938); C. 4% 



formaldehyde; D.2% gold chloride; E. water 100, 40% formaldehyde 1, oxalic acid 2; 



F. 5% sodium thiosulfate 

 method: [sections of formaldehyde-fixed material] —> A , 1-132 hrs. -^ rinse -^ B, 3 



mins.— » rinse — > C, 1 min. -^ wash -^ D, 10 mins. -^ wash -^ E, 20 sees. — > wash -^ 



F, 2 mins. — ^ wash -^ balsam, via usual reagents 

 recommended for: nerve trunk of oligochaetae. 



31.21 de No 1926 test. 1933 Cajal and de Castro Cajal and de Castro 1933, 368 



reagents required: A. 50% pyridine; B. 3% nitric acid; C. 0.1% ammonia; D. 15% 



pyridine in 50% ale; E. 2% silver nitrate; F. AMS 21.1 Cajal 1910 

 method: [fresh tissue] -^ A, 24 hrs. -^ wash, 24 hrs. -^ B, till decalcified — > wash — » C, 



24 hrs. -^ D, overnight -^ E, 6-7 days, 37°C. -* wash -^ F, 24 hrs. -^ wash -> [cel- 



loidin sections] 

 RECOMMENDED FOR: nerve endings in structures requiring decalcification. 



31.21 Okada 1929 8542a, 7:403 



REAGENTS REQUIRED: A. 0.02% sodium hydroxide in abs. ale; B. 1.5% silver nitrate; 



C. AMS 21.1 Okada 1929; D. 5% sodium thiosulfate 

 method: [1 mm. slices of fresh tissue] -^ A, 6-12 hrs. -^ thorough wash — > B, 3-5 days, 



37°C. -^ C, 1-2 mins. -> [paraffin sections] -^ D, 15 mins. -^ wash -^ balsam, via 



usual reagents 

 recommended for: neurofibrils and pericellular net. 



31.21 Perez 1931 21344,27:187 



reagents required: A. 15% chloral hydrate; B. 0.2% ammonia in abs. ale; C. 1.5% 



sUver nitrate; D. ADS 21.1 Cajal 1910a 

 method: [2 mm. slices of fresh skin] — > A, 24 hrs. — > rinse -^ B, 24 hrs. — > wash, till 



rehydrated -* C, 7 days, 37°C. -^ wash -^ D, 24 hrs. -* wash -^ [paraffin sections] 

 recommended for: Meissner's bodies in human skin. 



31.21 Podhradszky 1934 23632, 50:285 



. reagents required: A. MS 31.1 Podhradszky 1934; B. AMS 21.1 Podhradszky 1934; 

 C. 1% gold chloride in 95% alcohol; D. 5%, pyrogallic acid in 95%, alcohol 

 method: [5 n sections of F 0000.1010 Podhradszky 1934 fixed material, varnished to 

 slide with collodion] -^ 80% ale. -^ A, in dark, 12 hrs., 15°C. -^ 1 hr., 37°C. -^ 95% 

 ale, rinse -^ B, till reduced -^ 96 %o ale, wash -^ C, till gray -^ 95 %o ale, wash -^ D, 

 3 mins. — > abs. ale, rinse -^ balsam, via xylene 



31.21 Rachmanov test. 1946 Roskin Roskin 1946, 257 



REAGENTS REQUIRED: A. 10% silver nitrate; B. AMS 21.1 Rachmanov (1946); C. 5% 



sodium thiosulfate 

 method: [sections of alcohol-fixed material] —> water ^ A, 24 hrs., 37°C. in dark -^^ 



wash — > B, 1-3 mins. — > wash -^ C, 3-5 mins. — * balsam, via usual reagents 



31.21 Ranson 1914 766,46:522 



reagents required: A. 1% ammonia in 95% ale; B. pyridine; C. 2% silver nitrate; 



Z). AMS 21.1 Ranson 1914 

 method: a, 48 hrs. -^ rinse -^ B, 24 hrs. -» C, 3 days, 35°C., in dark -^ rinse -^ D, 24 



hrs. -^ [sections by paraffin technique] 



31.21 Rasmussen 1938 7802, 23:263 



reagents required: A. 0.2% ammonia in abs. ale; B. 3% nitric acid; C. 0.15% am- 

 monia in 80% ale; D. pyridine; E. 2% silver nitrate; F. 0.75% silver nitrate; G. 4% 

 pyrogallol 

 method: [small pieces fresh hypophysis]—* A, 2-3 days ^ running water, 12 hrs.-* 

 B, 2 hrs. -^ rinse -> 80% ale, several changes, few hrs. -^ C, overnight -> D, 12 hrs 

 -* thorough wash ^^ E, in dark, 24 hrs. -* F, in dark, 24 hrs. -^ E, in dark, 24 hrs. -^ 

 rinse — * G, 24 hrs. — > wash -^ [paraffin sections] 

 recommended for: nerve fibers in hypophysis. 



