MS 31.21 METAL STAINS 557 



31.21 Sand 1910 6593, 12:128 



REAGENTS REQUIRED: A. 10% iiitric aci(l in acetone; B. 20% silver nitrate; C. AMS 21.1 



Sand 1910; D. AMS 23.1 Dand 1910; E. 5% sodium thiosulfate 

 method: [small pieces fresh tissue] — > A, 1 hr. — > A, fresh solution, 24 hrs. — > A, fresh 



solution, 24 hrs. ^ [sections by paraffin technique, using acetone for dehydration; 



bring sections to water, via acetone] —* B, S hrs., 37°C. —^ C, 10 mins. — > rinse — > D, 5 



mins. — > wash -^ E, 15 sees. — > wash -^ balsam, via usual reagents 

 note: This method was republished later (Sand 1915: 4349, 5:71). 



31.21 Schultze test. 1948 Romeis Romeis 1948, 418 



REAGENTS REQUIRED: A. 0.5% sodium liydroxidc; 5. 2% silver nitrate; C. water 95, 



AMS 21.1 Schultze (1948) 5 

 method: [30-40 ^ frozen sections of formaldehyde-fi.xed material] — » wash -^ A, \ day 



— > wash, till wash water alkali-free (test with phenolphthalein) — ^ B, lG-24 hrs, — > 



C, till completely reduced -^ wash -^ balsam, via usual reagents 

 recommended for: nerve bundles in cerebrum. 

 METHOD FOR CELLS IN CEREBRUM: Substitute 0.04% sodium hydroxide for A and 0.5% 



silver nitrate for B. 



METHOD FOR MEDULLA, AND CEREBRAL, SPINAL, AND SYMPATHETIC GANGLIA: Substitute 



0.8% sodium hydroxide for A and 10% silver nitrate for B. 

 METHOD FOR CEREBELLUM: Substitute 0.16% sodium hydroxide for A and 0.25% silver 



nitrate for B. 

 METHOD FOR PERIPHERAL NERVES: As for medulla and ganglia, but with a 1% dilution of 



AMS 21.1 Schultze (1948) substituted for C. 

 note: The methods of Lobe 1937 {lest. Romeis 1948, 420) are essentially the same save 



that MS 33.1 Lobo 1937 is substituted for B, above, and that A is used at 60°C. for 



15 minutes. 



31.21 Schultze and Stohr test. 1933 Cajal and de Castro 



Cajal and de" Castro 1933, 198 

 reagents required: A. 0.8% sodium hydroxide; B. 2% silver nitrate; C. ADS 21.1 



Schultze and Stohr 1933; D. Any AMS 22.1 formula 

 method: [frozen sections of formaldehyde-fixed material] — > A, 1 day -^ wash, 1 hr. — > 



B, overnight -^ C, till reduced -^ D -^ balsam, via usual reagents 

 recommended for: nerve fibers in sections of nerve tissue. 



31.21 Tello 1932 test. 1933 Cajal and de Castro Cajal and de Castro 1933, 369 



REAGENTS REQUIRED: A. 50% pyridine; B. AF 21.1 Tello 1932; C. 1% ammonia in 95% 



ale; D. 1.5% silver nitrate; E. AMS 21.1 Cajal 1910 

 method: [fresh tissue] -^ A, 24 hrs. — ♦ wash, 24 hrs. -^ B, till decalcified -^ wash, 24 



hrs. ^ C, 24 hrs. -^ D, 5-7 days, 37°C. -^ wash -^ E, 24 hrs. -^ wash -^ [celloidin 



sections] 

 recommended for: nerve endings in structures requiring decalcification. 



31.21 Uyama 1926 8542a, 4:389 



reagents required: A. 3% silver nitrate; B. AMS 21.1 Uyama 1926 



method: [washed, enucleated, eye] ^ A, 3-5 days, 37°C. -+ rinse -> B, 12-36 hrs. -» 



wash — > [paraffin sections] 

 recommended for: nerve net in retina. 



31.21 Walgren 1930 test. 1946 Roskin Roskin 1946, 232 



REAGENTS REQUIRED: A. AMS 11.1 Cajal 1933a; B. 1% silver nitrate; C. AMS 21.1 



Cajal 1910; D. 0.05% potassium permanganate in 0.1% sulfuric acid; E. 1% oxalic 



acid 

 method: [fresh fragments]-^ A, 5-9 days—* rinse -^ B, 1-2 days — > wash—* C, 1-2 



days —> paraffin sections — > water — > D, few moments — > rinse — » E, few moments — > 



[repeat D —* E cycle till differentiation complete] — * wash — * balsam, via usual 



reagents 



31.21 Willard 1935 17510,78:475 



REAGExNjTS required: A. AMS 13.1 Willard 1935; B. 2.5% silver nitrate; C. AMS 21.1 

 Willard 1935 



