558 METHODS AND FORMULAS MS 31.22-MS 31.31 



method: [fresh tissue] -^ A, 24 hrs. -^ wash — ♦ 96% ale, 24 hrs. -^ wash -* B, 9-12 

 days, 37.5°C. -^ rinse -^ C, 12-24 hrs. —> [15 m to 30 m sections by paraffin techniques] 

 RECOMMENDED FOR: innervation of adrenal. 



31.22 NEUROGLIA 



31.22 Boisi 1927 test. 1933 Cajal and de Castro Cajal and de Castro 1933, 274 



REAGENTS REQUIRED: A. AMS 11.1 Cajal 1914; B. AMS 11.1 Noguchi 1913; C. AMS 13.1 



Bolsi 1927; D. 2% silver nitrate; E. AMS 24.1 Bolsi 1927; F. 5% sodium thiosulfate 



in 50% ale. 

 method: [pieces of fresh tissue]—* A, 24-48 hrs. — > B, 1 or more months^ wash—* 



[frozen sections] — * water -* B, 10 mins. 45°-50°C. —> cool -^ C, 5 mins. — » Z), 5 mins. 



— > £■, 5 mins. —* F, b mins. -^ balsam, via carbol-xylene 

 RECOMMENDED FOR: microglia. 



31.22 Cajal 1925 test. 1933 ips. Cajal and de Castro 1933, 262 



REAGENTS REQUIRED: A. AMS 11.1 Cajal 1913; B. 5% formaldehyde; C. MS 31.1 Cajal 



1925; D. 0.2% gold chloride; E. 5% sodium thiosulfate 

 method: [pieces of fresh tissue] -^ A, 24 hrs. — > B, till required — > [frozen sections] —>■ 



C, till dark chestnut, 5-10 mins. -^ wash — » D, till violet —^E,5 mins. -^ wash — > 



balsam, via usual reagents 

 RECOMMENDED FOR: macroglia and microglia. 



31.22 Lobe 1937 test. 1948 Romeis Romeis 1948, 420 



REAGENTS REQUIRED: A. AMS 12.1 Lobo 1937; B. 4% formaldehyde; C. 0.04% sodium 



hydroxide; D. MS 31.1 Lobo 1937; E. AMS 21.1 Lobo 1937; F. 0.2% gold chloride; 



G. 5% sodium thiosulfate 

 method: [fresh tissue] ^^ A, (see note below for time) -^ B, till required—* [30-40 n 



frozen sections] -* wash — > C> 5-8 mins., 60°C. — * wash, till wash water alkali-free 



when'phenolphthalein tested -* D, overnight, 60°C. — * rinse — * E, 3-5 mins. -^ wash —* 



F, 10-15 mins. -^ G, 5 mins. -^ balsam via usual reagents 

 RECOMMENDED FOR: microglia (3-5 days in ^1), protoplasmic glia (15-30 days in A). 



31.22 Merland 1936 4285a, 12 :290 



REAGENTS REQUIRED: A. AMS 11.1 Merland 1935; B. 10% silver nitrate; C. AMS 21.1 



Merland 1935; D. 10%, sodium thiosulfate 

 method: [frozen section of F 8000.1000 Merland 1935 material]-* A, changed fre- 

 quently, till silver nitrate test shows all bromide eliminated -^ A, fresh solution, 40 

 mins., 56°C. -^ rinse -* B, 30-60 mins. 5G°C. (till ochre) -* wash -* C, till reduced 

 -^ D, 5 mins. — * balsam, via usual reagents 

 RECOMMENDED FOR: astrocytes. 



31.3 Cytological Methods 



31.31 golgi apparatus 



31.31 Cajal 1912 see MS 31.31 Cajal 1914 (note) 



31.31 Cajal 1914 21344, 12:127 



REAGENTS REQUIRED: A. AMS 11.1 Cajal 1914; B. 1.5% silver nitrate; C. AMS 21.1 



Cajal 1914 

 method: [Small pieces of fresh tissue]-* A, 10-14 hrs.-* rinse -^ B, 36-48 hrs. -^ 



rinse -^ C, 8-24 hrs. — » wash —> [paraffin sections by usual techniques] 

 RECOMMENDED FOR: Golgi network. 

 note: This method is assigned, without reference, to Cajal 1912 by Cajal and de Castro 



1933, 203. 



31.31 Golgi 1908a test. 1933 Cajal and de Castro Cajal and de Castro 1933, 201 



reagents required: A. F 8000.1000 Golgi 1908; B. 1% silver nitrate; C. AMS 21.1 

 Cajal 1914; D. AMS 22.1 Golgi 1908; E. AMS 23.1 Golgi 1908; F. 1% oxalic acid 

 method: [fresh tissue] -* A, 6-8 hrs. — * wash -^ B,3 hrs. to some days -* quick rinse — > 

 C, 12 hrs. -^ wash — > 3 to 5 m sections -^ D, 10-30 mins. -* E, till differentiated -^F, 

 to stop differentiation —> [counterstain, if desired] -^ balsam, via usual reagents 

 RECOMMENDED FOR: Golgi network. 



