MS 31.31-MS 31.41 METAL STAINS 559 



31.31 Golgi 1908b /< s^ 1933 Cajal and de Castro Cajal and de Castro 1933, 201 



REAGENTS REQUIRED: .4. AMS 11.1 Cajal 1908; B. AMS 12.1 Cajal 1910; C. 2% silver 



nitrate; D. AMS 21.1 Cajal 1910 

 method: [pieces of tissue] —> A,24 hrs. -^ wash, 4-6 hrs. —>■ B, 24 hrs. -> quick rinse -> 



C, 5 days, 37.5°C. -> quick wash -* D, till reduced -^ [sections] 

 RECOMMENDED FOR: Golgi network. 



31.31 Rojasl917 21344, 15:30 



REAGENTS REQUIRED: A. AMS 11.1 Rojas 1917; B. 1.5% silver nitrate; C. AMS 21.1 



Rojas 1917 

 method: [fresh nerves] —> A, 24 hrs. -^ running water, 24 hrs. -> B, 48 hrs. -^ wash -^ 



C, 24 hrs. —>■ wash -^ tease -^ glycerol 

 RECOMMENDED FOR: demonstration of Golgi apparatus in wholemounts. 



31.31 Weatherford tesl. 1938 Mallory Mallory 1938, 113 



REAGENTS REQUIRED: A. F 8000.1000 da Fano 1920; B. 5% trichloracetic acid; C. 1.5% 

 silver nitrate; D. AMS 21.1 Cajal 1910; E. 0.2% gold chloride; F. 25% sodium 

 thiosulfate 



method: [fresh tissue]^ A, 6-8 hrs. — > wash ^ B, changed daily till ammonium ox- 

 alate calcium test is negative -^80% ale. thorough wash — » A, 1 hr. -^ quick wash — > 

 C, 36-48 hrs. — > rinse -> D, 8-24 hrs. in dark -^ wash — > [4-8 m sections] -^ water -^ 

 E, 5-10 mins. — > F, 10-15 mins. -^ [DS 11.21 counterstain, if required] —^ balsam, via 

 usual reagents 



RECOMMENDED FOR: Golgi bodies in tissues requiring decalcification. 



31.32 OTHER CYTOLOGIC AL METHODS 



31.32 Chatton and Lwoff 1930 6630, 104:834 



REAGENTS REQUIRED: .4. F 80000.1000 da Fano 1920 100, sodium chloride 1; E. V 22.1 



Chatton and Lwoff 1930; C. 3% silver nitrate 

 method: [live protozoa, concentrated on slide] -^ A, on slide, 5 mins. ^^ drains B, 



1 drop at 25°C. -^ cool to solidify -^ C, on slide, 5 mins. in dark -^ rinse -^ bright 



light till sufficiently reduced — ♦ wash — > balsam, via usual reagents 

 RECOMMENDED FOR: impregnation of basal bodies and nerve net in Ciliata. 

 note: Chatton and Lwoff 1940 (6630, 134:229) recommend development in 0.1% hydro- 



quinone. 



31.32 Kingsbury and Johannsen 1927 Kingsbury and Johannsen 1927, 83 



REAGENTS REQUIRED: .4. AMS 11.1 Kingsbury and Johannsen 1927; BA% silver nitrate; 



C. AMS 21.1 Kingsbury and Johannsen 1927 

 method: [small pieces of fresh tissue] —>■ A, 8-12 hrs. — > rinse -^ B, 24 hrs. —>■ wash — > C, 



24 hrs. -^ [wholemount or section] 

 recommended for: demonstration of invertebrate striped muscle. 



31.4 Histological IMethods 

 31.41 reticular fibers 



31.41 Cajal 1907 test. 1933 ips. Cajal and de Castro 1933, 290 



reagents required: A. AMS 13.1 Cajal 1907; B. water 50, 95% ale. 50, ammonia 0.3; 



C. 1.5% silver nitrate; D. AMS 21.1 Cajal 1910 

 method: [pieces of fresh tissue] -> A, 24 hrs. — > running water, 24 hrs. —> B, I day -» 

 rinse -> C, 5 days, 37°C. -^ wash -y D, 24 hrs. -^ wash -> [sections] 



31.41 Cajal test. 1933 ips. Cajal and de Castro 1933, 290 



reagents required: A. AMS 11.1 Cajal 1933; B. 0.3% ammonia in 95% ale; C. 2% 



sUver nitrate; D. AMS 21.1 Cajal 1910 

 method: [pieces of fresh tissue] -+ A, 24 hrs. -^ thorough wash — > B, 24 hrs. -> wash 



— > C, 3-4 days, 37°C. -^ wash, 1 min. -* D, 24 hrs. — > wash -^ [celloidin sections] 



