560 METHODS AND FORMULAS MS 31.42-MS 31.51 



31.42 OTHER HISTOLOGICAL METHODS 



31.42 Lauda and Rezek 1928 22575, 269:218 



REAGENTS REQUIRED: A. 0.25% ammonia in 95% alcohol; B. MS 31.1 Lauda and Rezek 



1928; C. AMS 21.1 Lauda and Rezek 1928 

 method: [fresh tissue]-^ A, 24 hrs. — > wash -^ B, 3-4 days — > wash—* C, 24 hrs. -^ 



wash — > 95% alcohol-^ [paraffin sections] 

 RECOMMEXDED FOR: general histology of kidney. 



31.42 Lillie 1928 23632, 45 :380 



REAGENTS REQUIRED: A. 2.5% silvcr nitrate; B. AF 21.1 von Ebner (1891b); C. 18% 



sodium chloride 

 method: [formaldehyde-fixed bones with fixative thoroughly washed out] — > A, 37°C., 

 4-5 days ^ thorough wash -^ B, till decalcified — > C, till acid-free —* [paraffin sec- 

 tions] 

 recommended for: structure of bone. 



31.42 Kossa 1901 2526, 29:163 



reagents required: A. 5% silver nitrate; B. 1% pyrogallol; C. 5% sodium thiosulfate 

 method: [frozen sections of undecalcified material] —* water — > A, 10-60 mins., in strong 



light — » wash —> B, 1-3 mins. — > rinse — * C, 3-5 mins. — > thorough wash — > balsam, 



via usual reagents 



31.42 Kossa test. 1933 Cajal and de Castro Cajal and de Castro 1933, 315 



reagents required: A. 0.2% silver nitrate; B. any DS 11.42 solution 

 method: [paraffin sections]—* water -^ A, J2 hr. — * wash — > B, till counterstained — > 

 balsam, via usual reagents 



31.5 Bacteriological Methods 



31.51 methods for spirochetes 



31.51 Armuzzi and Stempel 1924 test. 1928 Schmorl Schmorl 1928, 40b 



reagents required: A. pyridine: B. 5% uranium sulfate; C. 2% silver nitrate; D. AMS 



21.1 Armuzzi and Stempel 1924 

 method: [frozen sections of formaldehyde-fixed material] -* A, 1^^ hrs. —> wash — > 95% 

 ale, 1 hr. -^ water, 1 min. — > 5, 2 hrs. — > rinse -^ C, 2)^-3 hrs., 37°C. — > rinse -^ D, 

 10 mins. -* thorough wash -^ balsam, via usual reagents 



31.51 Bauer 1944 608b, 20 :297 



reagents required: A. MS 31.1 Bauer 1944; B. AMS 21.1 Bauer 1944 

 method: [blocks of formaldehyde-fixed and nitric acid-decalcified jaws] — * running water 

 wash, 2 daj's —>■ A, changed when cloudy, 18 days, in dark, 37°C. —> wash, 1 day — > B, 

 changed when cloudy, 48 hrs. in dark -* wash — ♦ [paraffin sections] 

 recommended for: spirochetes in tooth buds and jaws. 



31.51 Cajal test. 1933 ips. Cajal and de Castro 1933, 192 



reagents required: .1. 5% formaldehyde; B. MS 31.1 Cajal 1929; C. AMS 21.1 Cajal 



1910 

 method: [fresh tissues] — > A, 2 days ^95% ale, 24 hrs. — * fi, 2 days, 37°C. — > wash — > 



C, 24 hrs. — + [section] 

 recommended for: spirochetes in sections. 



31.51 Cajal and de Castro 1933 see MS 31.51 Noguchi (1913) (note) 



31.51 Dieterle 1927 1879, 18:73 



reagents required: A. 1% uranium nitrate in 70% ale; B. 10% gum mastic in abs. 



ale; C. 1% silver nitrate; D. AMS 21.1 Dieterle 1927 

 method: [sections, preferably of formaldehyde-fixed material] — >• A, 30 mins., 55°C. — * 

 wash — > 90% alcohol, 2 mins. —* B, 30 sees. -^ drain — > rinse, 90% alcohol —> rinse, 

 water —> C, 1 to 6 hrs., 55°C. in dark —>■ wash -^ D, 5-15 mins. -^ wash — * 96% alco- 

 hol till all traces of B removed — * balsam, via acetone 

 recommended for: spirochetes in sections. 



