MS 31.51 METAL STAINS 5G1 



31.51 Eyene and Sternberg test. 1916 Warthin 4349, 6:71 



REAGENTS REQUIRED: ,4. 1% silvop iiitiatc; />'. AMS 21.1 EyciH! and Sternberg (1916); 



C. 10% sodium thiosulfate 

 method: [thin sections of formaldehyde-fixed material] — > wash — > ^4, 30 mins., in dark, 

 37.5°C. — > B, 1-2 mins. -^ C, 1-2 mins. -^ wash — » balsam, via usual reagents 



31.51 Farrier and Warthin 1930 623, 14:394 



REAGENTS REQUIRED: .4. 1% silver nitratc at pll 4. 1; B. AMS 21.1 Farrier and Warthin 



1930; C. 5% sodium thiosulfate 

 method: [sections of formaldehyde-fixed material] — > A, 30 mins., 37°C., in dark -^ B, 

 till brownish black — > C, 5 mins. — » wash — > balsam, via usual reagents 



31.51 Hertzman lest. 1938 Mallory Mallory 1938, 293 



REAGENTS REQUIRED: .1. pyridine; B. 1% uranium nitrate; C. 0.25% silver nitrate; D. 



AMS 21.1 Hertzman 1938 

 method: [frozen sections of formaldehyde-fixed material] ^ ^, 10 mins. — > wash — > B, 



15 mins., 37°C. -^ rinse -^ C, 15-30 mins., 50°-60°C. -^ D, till dark brown, 50°-60°C. 



— > warm water, wash — » balsam, via usual reagents 



31.51 Jahnel test. 1933 Cajal and de Castro Cajal and de Castro 1933, 384 



REAGENTS REQUIRED: .4. pyridine; 5. 4% formaldehyde; C. 1% uranium nitrate; D. 



1.5% silver nitrate; E. AMS 21.1 Noguchi 1913 

 method: [2-4 mm. slices of alcohol- or formaldehyde-fixed material] — > A, 1-3 days — ♦ 



thorough wash -* B, 24 hrs. -^ C, 1 hr., 37°C. -> wash -» D, 5-8 days in dark, 37.5°C. 



— + wash -^ E, 1-2 days in dark — > [paraffin sections] 

 note: see also MS 31.51 Levaditi 1905 (note). 



31.51 Krajian 1935 1829,32:764 



REAGENTS REQUIRED: A. 1% sodium cobaltinitrite ; B. AMS 12.1 Krajian 1933; C. 0.75% 



silver nitrate; D. AMS 21.1 Krajian 1933 

 method: [frozen sections of formaldehyde material] -^ A, 5 mins. -^ wash ^ 5, 15 



mins., 67°C. -^ wash ^ C, 1 hr., 67°C. — > rinse — > C, 15-25 sees. — > wash — > balsam, 



via usual reagents 

 note: see also MS 33.51 Krajian 1933. 



31.51 Krajian 1938 1829,38:427 



REAGENTS REQUIRED: A. AMS 21.1 Krajian 1933; B. 0.5% mastic in 95% alcohol; C. 1% 



silver nitrate; D. AMS 21.1 Krajian 1933 

 method: [air-dried smears of exudate] -^> A, 5 mins., 37°C. -^ wash ^ B, poured on 

 slide — > drain and breath on surface till cloudy — > rinse — ^ C, on slide warmed to 

 bubbling, 3 mins. -^ C (repeat) — » drain -^ D, 2 mins. — * wash — > dry 



31.51 Krantz 1924 14074, 608 



REAGENTS REQUIRED: A. 0.1% silvcr nitrate; B. AMS 21.1 Krantz 1924 

 method: [sections of formaldehyde-fixed material] —> water —> A, 24 hrs., 00°C. — » 

 wash — ♦ B, 30-60 mins. — » wash -^ balsam, via usual reagents 



31.51 Knowles, Gupta, and Basu 1932 test. 1938 Hunter 



20540b, 13 :46 

 REAGENTS REQUIRED: A. 0.3% silver nitrate; B. 1% hydroquinone 

 method: [1 mm. slices of formaldehyde-fixed material, washed free of fixative]—* A, 



37°C., 24 hrs. —* wash, till free from silver -^ B, 24 hrs. — > [paraffin sections] 

 RECOMMENDED FOR: Spirochetes in avian tissues. 



31.51 Levaditi 1905 6630,58:845 



REAGENTS REQUIRED: A. 4% formaldehyde; B. 2% silver nitrate; C. AMS 21.1 Levaditi 



1905 

 method: [fresh tissue] —> A, 24 hrs. or till required -^^ 90% ale, 24 hrs. -^ water, till 



rehydrated — > B, 3-6 days, 37°C. —^ rinse -^ C, 1-3 days in dark -^ wash — > [5 m 



paraffin sections] 

 note: Schmorl 1928, 403 recommends the substitution of AMS 21.1 Levaditi and 



Manuelian 1906 for C above; .Jahnel test. 1933 Cajal and de Castro 384 recommend 



the substitution of AMS 21.1 Jahnel 1933 for C above. 



