MS 31.51-MS 31.6 METAL STAINS 563 



method: [10 M paraffin sections] — » abs. ale. -^ A, l-V/i mins. -^ thorough wash -^ B, 

 1-13'2 hrs., 100°C. — > abs. ale. via graded alcohols — » C, 5 mius. — > wash -> /), 5 min.s. 

 -^ [counterstain, if desired] — » balsam, via usual reagents 



31.51 Steiner and Steiner 1944 11284,29:868 



REAGENTS REQUIRED: A. 1% uranium nitrate; B. 1% silver nitrate; C. 2.5% gum mastic 



in abs. ale; D. AMS 21.1 Steiner and Steiner 1924 

 method: [paraffin sections] —> water —> A, 3 mins. — > wash—* B, 2 hrs., 56°-58°C. -^ 



wash -^ dehydrate —* C, 5 mins. — » drain —* D, 12-15 mins. — » wash — > balsam, via 



usual reagents 



31.51 Warthin 1916 4349,6:71 



reagents required: A. 2% silver nitrate; B. AMS 21.1 Levaditi 1905 



method: [pieces of F 8000.1000 Warthin 1916 fixed, and alcohol-hardened, material) — ♦ 



wash — > 4, 2 days in dark, 37.5°C. -+ wash, in dark — > B, 48 hrs., in dark — ♦ wash —* 



[paraffin sections] 



31.51 Warthin-Starry 1929 test. Langeron 1942 Langeron 1942, 629 



reagents required: A. 1% nitric acid; B. 2% silver nitrate; C. AMS 21.1 Warthin- 

 Starry 1929; D. 5% sodium thiosulfate 

 method: [sections of formaldehyde-fixed material on slides]-^ A, 1-30 mins. — > rinse 

 — » B, 30 mins. to 2 hrs., 55°C., in dark, by dipping section bearing slide into stain, 

 placing another slide on top and laying this sandwich in half its depth of stain — » 

 remove cover slide, lay section bearing slide, sections up, in 3 mm. layer C till brown 

 —> wash, warm water — > Z), 2 mins. -^ wash — > balsam, via usual reagents 

 recommended for: spirochetes in section. 



31.51 Yamamoto 1909 23681,20:153 



reagents required: ^1. 4% formaldehyde; B. 5% silver nitrate; C. AMS 21.1 Yama- 

 moto 1909 



method: [thin slices] — > ^4., 24 hrs. — » 95% ale, 1 hr. — > running water, 24 hrs. — ♦ dist. 

 water, 1 hr. —> B, 47 hrs., 37°C. — > rinse —> C, changed after 1 hr., and whenever 

 turbid, 24 hrs., 37°C. -^ wash — > [celloidin sections] 



31.52 OTHER BACTERIOLOGICAL METHODS 



31.52 van Ermengen 1894 23684, 15 :969 

 REAGENTS REQUIRED: .1. 1% silvcr nitrate; B. AMS 21.1 van Ermengen 1894 

 method: [bacterial smears, fixed 30 mins. in F 1000.0019 van Ermengen 1894] — > water, 



wash ^^95% ale., wash -^ A, on slide, 15-30 sec. — > B, added to A on slide, 30 sees. — > 

 A, dropped on mixture on slide till ppt. occurs — > water, wash — > dry 

 recommended for: demonstration of bacterial flagella. 



31.52 Steiner 1950 591b, 20:489 



REAGENTS REQUIRED: A. AMS 12.1 Steiner 1950; B. 0.1% sUver nitrate; C. 2% mastic in 



abs. ale.; D. 5% pyrocatchol 

 method: [8 n paraffin sections of formaldehyde-fixed tissues] -^ abs. ale. -^ A,5 mins. — »• 



water, changed till no longer milky -^ B, 14-16 hrs., 60°C. -^ thorough wash — » abs. 



ale. via graded ales. -^ C, 5 mins. — > D, 1 hr., 60°C. — > wash —> balsam, via usual 



reagents 

 recommended for: microorganisms in tissues. 



31.6 Other Silver Nitrate Methods 



31.6 Gomori 1940 16913,44:250 



REAGENTS REQUIRED: A. 0.5% silver nitrate; B. 5% sodium acid phosphite; C 2% 



sodium thiosulfate 

 method: [alcohol-fixed teeth] -^ A, 1 day -^ wash, 1 day — > 5, 1 day — > wash -* C, 12 



hrs. -^ [sections] 

 recommended for: carious lesions in teeth. 



31.6 Hanazawa 1917 Dent. Cosinos, 59:125 



reagents required: A. 2% silver nitrate; B. any AMS 21.1 fornmla; C. 5% sodium 

 thiosulfate 



