MS 32.1 METAL STAINS 5G7 



32.1 Davenport, McArthur, and Bruesch 1939 20540b, 14:22 



REAGENTS REQUIRED: .1. 10% sUvcr nitrate; B. 0.2% protein silver; C. AMK 21.1 



Davenport, McArthur, and Bruesch 1939; D. 0.1% }j;ohl chloride; E. 1% diainino- 



phenol hydrochloride 

 method: [paraffin sections of material fixed 2 to 12 hrs. in F 0000.0023 Davenport, 



McArthur, and Bruesch 1939] -^ wash -^ A, 1 hr., 58-G2°C. -^ wash -^ B, 1 hr. -^ 



quick rinse — » C, 1 min. -^ wash —>■ D, till color gray — + wash — > E, dropped on slide, 



few sees. — > wash — ^ balsam, via usual reagents 

 RECOMMENDED FOR: general nervous histology. 



32.1 Davenport, Windle, and Rhines 1947 Conn and Darrow 1947 1C2, 24 



REAGENTS REQUIRED: A. 5% silvcr nitrate; B. 0.2% protein silver; C. AMS 21.1 Daven- 

 port, Windle, and Rhines 1947; D. 0.2% gold chloride; E. 0.4% oxalic acid; F. 5% 

 sodium thiosulfate 



method: [paraffin sections of material fixed in F 9000.4000 Davenport, Windle, and 

 Rhines 1947] — > water -^ A, 1 hr., 58-62°C. — > wash, 3 changes, 30 sees, each — > B, 

 1 hr. — » quick rinse — > C, 1 min. -^ wash, running water — > D, till gray — > rinse — > E, 

 15-45 sees, till darkening starts -^ rinse — >• F, 1-2 mins. — > wash — > balsam, via usual 

 reagents 



RECOMMENDED FOR: axis-cylindcrs. 



note: a detailed description of the application of this technique is given under MS 32.0 

 above. The method of Dawson and Barnett 1944 for argentophil granules differs from 

 the above in that the " B —* rinse —* C" cycle is repeated. 



32.1 Dublin 1944 1829,50:361 



reagents required: A. 1% protein silver; B.\% hydroquinone; C. 0.5% gold chloride; 

 D. 2% oxalic acid; E. 5% sodium thiosulfate; F. water 100, acetic acid 0.1, ponceau 

 2R 0.75; azophloxine 0.125; G. 0.1% acetic acid; H. water 100, acetic acid 0.1, phos- 

 photungstic acid 1.5, orange G 1; /. water 100, acetic acid 0.1, light green 0.02 

 method: [6-8 m sections of formaldehyde-fixed material] -^ water -^ yl, 2-4 hrs. ^ 

 rinse -^ B, 5 mins. — > rinse -^ C, till gray —* rinse — * D, 5 mins. —> rinse -^ E, 5 

 mins. — * wash —* F, 5 mins. — > G, rinse —* H, 5 mins. time -^ balsam, via usual 

 reagents 

 recommended for: skin. 



32.1 Foley 1943 20540b, 18:27 



reagents required: A. water 50, 95% ale. 50, ammonia 1; B. 1% protein silver in dish 

 containing copper foil varnished with 0.5% celloidin; C water 50, 95% ale. 50, pro- 

 tein silver 0.5, pyridine 1 in dish containing copper foil varnished with 0.5% celloidin; 

 D. AMS 21.1 Foley 1943; E. 0.2%, gold chloride in 0.1% acetic acid; F. 2% oxalic 

 acid; G. 5% sodium bisulfite; H. DS 11.41 Einarson 1932; /. 5% phosphotungstic 

 acid; /. water 97, acetic acid 3.2, anilin blue 0.004, fast green FCF 0.2, orange G 0.8 

 method: [25 n frozen sections of formaldehyde-fixed material] —* A, 24 hrs. — > B, 6-8 

 hrs., 37°C. -^ C, 1-2 days, 37°C. -^ 50% ale, rinse -^ D, 10 mins. -^ wash -^ E, 10 

 mins. — > F, 1-3 mins. — > rinse —> G, 3-5 mins. — > wash -^ H, overnight — » wash — >■ 

 7, 30 mins. -^ J, 1 hr. — > 95% ale, till differentiated — > balsam, via butyl alcohol and 

 cedar oil 



32.1 Humphreys 1939 608b, 15:151 



reagents required: A. AMS 12.1 Humphreys 1939; fi. 2% protein silver (in jar con- 

 taining 10 Gm. metallic copper per 100 ml.); C. AMS 21.1 Humphreys 1939; D. \% 

 gold chloride; E. water 99, 40% formaldehyde 1, oxalic acid 2 

 method: [blood vessels dissected from formaldehyde-fixed material] -^ thorough wash 

 —^ A, 4: hrs. -^ wash, 2 hrs. — > B, 6-24 hrs. — > wash — + C, till completely reduced — » 

 D, till gray -^ wash — > E, till blue —* balsam, via usual reagents 

 recommended for: perivascular nerves in wholemounts of intracerebral vessels. 



32.1 MacFarland and Davenport 1941 20540b, 16:53 



REAGENTS REQUIRED: A. 1% thallium nitrate; B. 1% protein silver; C 0.1% oxalic acid; 

 D. AMS 21.1 Bodian 1936; E. 0.2% gold chloride; F. AMS 21.1 MacFarland and 

 Davenport 1941; (?. 5% sodium thiosulfate 



