582 METHODS AND FORMULAS MS 33.21 



33.21 Davenport, Windle, and Beech 1934 20540b, 9:5 



REAGENTS REQUIRED: A. 50% pyridine; B. 1.5% silver nitrate; C. MS 33.1 Davenport, 



Windle, and Beech 1934; D. 0.4% formaldehyde 

 method: [embryos fixed 2 days in F 0000.1020 Davenport, Windle, and Beech 1934] -^ 



wash, 1 hr. ->■ A, 1-2 days -» wash, 2-4 hrs. -> i?, 3 days, 37°C. -^ wash, 20 mins. (12 



mm. embryos) to 1 hr. (20 mm. embryos) -^ C, 6-24 hrs. -^ wash, 15 mins. -» D, same 



time as C — » wash — > [section] 

 RECOMMENDED FOR: embryos. 



33.21 Debauche 1939a 20540b, 14:121 



REAGENTS REQUIRED: A. \% ammonia; JS. 2% ammonia; C. 20% silver nitrate; D. am- 

 monia; E. 1.2%, formaldehyde; F. 1% gold chloride; G. 5% sodium thiosulfate 



method: [tissue fragments fixed in F 5000.1010 Debaissieux 1935]-* A, 2-6 hrs. — > 

 [frozen 25 n sections] -^ B, 15 mins. -^ C, 45°C. till light brown -» D, added drop by 

 drop to C containing sections until ppt. first formed is not quite redissolved -^ rinse 

 — > E, till rich brown -^ F, till deep blue ^ G, 10 mins. — > wash — > balsam, via usual 

 reagents 



RECOMMENDED FOR: invertebrate neurology in free sections. 



note: This method was republished by the same author (1939: 966, 59:23). It may 

 be applied to sections cut by the paraffin technique, if the wax is removed and the 

 impregnation conducted before the section is attached to a slide. 



33.21 Debauche 1939b 20540b, 14:21 



REAGENTS required: A. 2% ammonia; B. 10% silver nitrate; C. MS 33.1 Debauche 



1939; D. 1.2% formaldehyde; E. 1% gold chloride; F. 5% sodium thiosulfate 

 method: [serial sections from E 21.1 Debauche 1939 blocks of F 5000.1010 Debaissieux 



1935 fixed material] -^ water — > A, 10 mins. — » quick rinse — > B, 10 mins. 45°C. — * 



C, 1-5 mins. -^ quick rinse -^ D, till rich brown — * E, till blue — > F, 5 mins. —* wash 

 -^ balsam, via usual reagents 



recommended for: invertebrate neurology in serial sections. 



33.21 Doinikow test. 1933 Cajal and de Castro Cajal and de Castro 1933, 341 



reagents required: A. 8% formaldehyde; B. pyridine; C. 2% silver nitrate; D. MS 



33.1 Bielschowsky 1902 

 method: [blocks of fresh tissue] — > A, some months — > wash — > B, 24-48 hrs. —> running 

 water, 24 hrs. — * distilled water, some hrs. — > C, 4-5 days, 35°C. -^ rinse —y D, 48 

 hrs. — + thorough wash -^ A, 48 hrs. — > wash — > [celioidin sections] 

 recommended for: regenerating nerve fibers. 



33.21 da Fano 1914 2190, 3:14 



REAGENTS REQUIRED: A. 2% sUver nitrate; B. MS 33.1 da Fano 1914; C. 8% neutralized 

 formaldehyde; D. 0.004% gold chloride; E. 5% sodium thiosulfate 



method: [small pieces, fixed in formalin or F 7000.1000 Orth 1896] -^> wash^ [sections 

 by freezing technique] — > wash -^ A, in dark, 6 hrs. to 3 days -^ B, 20 to 30 mins. —*■ 

 wash — > C, 15-20 mins. -^ wash — ^D, 1 hr. -^ E,2 mins. — * balsam, via usual reagents 



33.21 da Fano 1919 11454, 52:1919 



REAGENTS REQUIRED: A. pyridine; B. 2% silver nitrate; C. MS 33.1 da Fano 1919 33.1; 



D. 8% neutralized formaldehyde; E. 0.004% gold chloride; F. 5% sodium thiosulfate 

 method: [pieces, fixed 8% formaldehyde, 1-2 months] — > wash — + [sections by freezing 



technique] — ♦ wash — > A, 6-12 hrs. -^ wash -^ B, 24-48 hrs. -^ rinse -^ C, 15 to 20 

 mins. — »• rinse —> D, 2-3 hrs. -^ wash -^ E, \ hr. -^ F, 2 mins. — * balsam, via usual 

 reagents 



33.21 da Fano 1920 11454, 53:1919 



REAGENTS REQUIRED: A. pyridine; B. 9>% formaldehyde; C. 2% silver nitrate; D. MS 



33.1 da Fano 1919; E. 8% neutral formaldehyde; F. 0.004% gold chloride; G. 5% 



sodium thiosulfate 

 method: [pieces, fixed 8% formaldehyde, 1-2 months]^ wash—* [sections by freezing 



technique] — * wash —> 4, 4 to 12 hrs. -^ wash -^ B, 24 hrs. 37°C. — > C, 1-2 days —> rinse 



-^ D, 30 mins. -^ rinse — * E, 2-3 hrs. -^ wash — > F, 1 hr. —> G, 2 mins. -^ balsam, 



via usual reagents 



