MS 33.21 METAL STAINS 583 



note: The method above is the most complex of eight pubhshed in the same paper; 

 the others: (a) reverse steps .4 and B above; (b) substitute 60% methyl alcohol for A 

 above; (c) as b but with order of A and B reversed as in (a); (d) reverse steps A and 

 B, substituting AMS 11.1 da Fano 1920 for A; (e) substitute AMS 11.1 da Fano 1920b 

 for A; omit B; (f) substitute 50; pyridine for A; omit B; (g) omit A and B. 



RECOMMENDED FOR: neurofibrils (original and a) in adult (f) and embryonic (g) material, 

 general neurological staining (e), and pericellular baskets (b, c, d). 



33.21 Fajersztajn 1901 test. 1933 Cajal and de Castro Cajal and de Castro 1933, 223 



REAGENTS REQUIRED: A. MS 33.1 Fajersztajn 1901; B.2% formaldehyde; C. 0.2% gold 



chloride; D. 5% sodium thiosulfate 

 method: [frozen sections of formaldehyde material] -^ water -^ A, 5-10 mins. -^ B, few 



moments — > C, till violet —>^D,5 mins. — * wash — > balsam, via usual reagents 

 recommended for: axis cyUnders in ganglia, cerebellum, spinal cord. 



33.21 Favorsky 1906 see MS 33.21 Bielschowsky 1910 (note) 



33.21 Final 1947 6630, 141:198 



reagents required: A. 20% silver nitrate; B. 40% formaldehyde; C. MS 33.1 Gros 



(1938); D. 1% ammonia; E. 0.1% acetic acid 

 method: [paraffin sections of F 5000.1010 Bouin 1897] -> water -^ blot -^ A, 24 hrs., 

 37°C. — * blot — > B, on slide, 1-2 sees. — > blot -^ C, till sufficiently differentiated 

 about 30 sees. -^ D, wash — > E, rinse -^ balsam, via usual reagents 



33.21 Glees, Meyer, and Meyer 1946 11025, 80:101 



reagents required: A. 0.1% ammonia in 50% alcohol; B. 10% silver nitrate; C. 4% 



formaldehyde; D. MS 33.1 Glees, Meyer, and Meyer 1946; E. 5% sodium thiosulfate 

 method: [15 ju frozen sections of formaldehyde material] —* A, 24 hrs. —>■ wash -^ B, till 



brown, 1-5 days -^ C, 1 hr. — > wash — > D, 30-60 sees. —* C, 5 mins. — > wash -^ E, 10 



mins. — > wash — >• balsam, via usual reagents 



33.21 Gros-Schultze test. 1938 Mallory Mallory 1938, 227 



reagents required: A. 20% silver nitrate; B. 20% formaldehyde; C. MS 33.1 Gros- 

 Schultze 1938; D. 20% ammonia; E. 0.5% acetic acid; F. 1% gold chloride; G. 5% 

 sodium thiosulfate 

 method: [frozen sections of formaldehyde-fixed material] -+ A, in dark, 5-20 mins. — > 



B, 4 washes in 4 successive portions — > C, till axis cylinders differentiated — » D, rinse 

 — > E, rinse -^ F, 1 hr. —> G, 1 min. -^ wash -^ balsam, via usual reagents 



recommended for: nerve endings. 



note: Cajal and de Castro 1933, 178 give this technique as "Gros." Szatmari 1936 



(23639b, 24 :239) precedes A, above, with 24 hours soaking in pyridine which is then 



thoroughly washed out. 



33.21 Holmes 1942 11431, 54:132 



REAGENTS REQUIRED: A. Xylene 30, acetic acid 70; B. MS 33.1 Holmes 1942; C. AMS 21.1 



Holmes 1942; D. 0.2% gold chloride; E.2% oxalic acid; F. 5% sodium thiosulfate 

 method: [15 n paraffin sections of formaldehyde material]—* A, 1 hr. -^ water, via 

 graded ales. -^ B, 5-24 hrs. 37°C. —> rinse —y C, 30 sees. — > thorough wash -^ D, 3-5 

 mins. -^ rinse — > E, 5-10 mins. -> F, 5 mins. -^ wash -^ balsam, via usual reagents 



33.21 Kernohan 1930 21400a, 49:58 



reagents required: A. 20% silver nitrate; B. MS 33.1 Bielschowsky 1902 20, water 80; 



C. 4% formaldehyde; D. 0.2% gold chloride; E. 2% sodium thiosulfate 

 method: [sections either by paraffin or collodion techniques]—* wash—* A, 1 hr. ^ 



wash ^ B, 1 to 4 mins. —> wash — > C, 1 min. -^ D, 2 mins. —* E, 2 mins. -^ balsam 

 via usual reagents 



33.21 Landau 1940 4285a, 17 :65 



REAGENTS required: A. 10% neutralized formaldehyde; B. 20% silver nitrate; C. MS 

 33.1 Gros-Schultze (1938); D. 0.04% neutrahzed formaldehyde; E. 0.2% gold 

 chloride; F. 5% sodium thiosulfate; G. 10% potassium iodide 



