584 METHODS AND FORMULAS MS 33.21 



method: [paraffin sections of formaldehyde material]—* A, 24 hrs. in dark — > B, 1-2 

 hrs. 35°-40°C., in dark -^ rinse — > C, 5 mins. -^ add few drops D to C —> repeat addi- 

 tions of C till sections sufficiently stained — * thorovigh wash -^ E, till blue grey — >• F, 

 5 mins. -^ G, till differentiated —> wash —* balsam, via usual reagents, or M 11.1 

 Landau 1940 



33.21 Lobo 1937 see MS 33.21 Schultze (1948) (note) 



33.21 Miskolczy test. 1933 Cajal and de Castro Cajal and de Castro 1933, 341 



KEAGENTs requieed: A. 10% silver nitrate; B. MS 33.1 Cajal 1925; C. 0.5% acetic acid; 



D. 8% formaldehyde; E. 0.2% gold chloride 

 method: [blocks of formaldehyde fixed material] —> [graded ales, till dehydrated]-^ 



xylene, 2-3 hrs. -^ [graded ales., till rehydrated] -^ water — > A, 2-7 days -^ wash —>■ 



B, 2-5 hrs. -^ wash — > C, 10-30 mins. — > wash — > D, 24 hrs. — * wash —>■ [paraffin sec- 

 tions on slide] — > water —* E, 5-10 mins. — » wash -^ balsam, via usual reagents 



recommended for: axis cylinders. 



33.21 Paton 1907 14246, 18:576 



REAGEXT-s REQUIRED: A. 4% neutralized formaldehyde; B. 1% silver nitrate; C. MS 33.1 



Bielschowsky 1902; D. 2% acetic acid; E. AMS 21.1 Paton 1907; F. 0.004% gold 



chloride; G. 5% sodium thiosulfate 

 method: [whole fish embryos] —'■A, 1 to 5 days —>■ wash — > B, 4 to 7 days — > rinse — > 



C, 24 hrs. -^ wash — > Z), 5 to 15 mins. — > wash —>■ E, 12 hrs. — > [sections by paraffin 

 technique, bring sections to water] —> F, I hr. — > G, few minutes -^ balsam, via usual 

 reagents 



33.21 Penfield 1935 608b, 11:1007 



REAGENTS REQUIRED: A. watcr 50, citric acid 10.5, 40% formaldehyde 50; B. 20% silver 

 nitrate; C. 20% formaldehyde; D. MS 33.1 Penfield 1935; E. 20% ammonia; F. 5% 

 acetic acid; G. any AMS 22.1 formula; H. 5% sodivmi thiosulfate 

 method: [small blood vessels dissected from material perfused for 3 days with A]~^ 

 wash -^ B, 2 hrs. -^ C, 4 changes, each 100 ml. -^ D, till stained — > E, 1-2 mins. -^ 

 F, till neutral — > G, till toned — > H, 10 mins. —> wash -^ l^alsam, via usual reagents 

 RECOMMENDED FOR: perivascular nerves of pia mater. 



33.21 Pullinger 1943 11431, 56:97 



REAGENTS REQUIRED: A. 0.1% ammouia; B. MS 33.1 del Rio-Hortega 1917a; C. 4% 



formaldehyde 

 method: [cornea fixed in situ by injection of formaldehyde, 1 day, then cut from eye and 



fixed 3 more days] — > wash — > A, overnight—* wash—* B, 37°C., 4 hrs. — > wash—* 



C, 15 mins. — ^ [sections] 



33.21 Rogers 1931 763,49:81 



REAGENTS REQUIRED: A. 10% formaldehyde; B.3% ammonia in 90% ale; C. 40% silver 



nitrate; D. MS 33.1 Rogers 1931; E. 4% acetic acid: F. 0.3% gold chloride in 1% 



acetic acid; G. i% oxalic acid; H. 5% sodium thiosulfate 

 method: [fresh tissue] — ♦ ^4, 1 wk. —>■ wash —^B,! day — * [paraffin sections] — * C, 13 hrs. 



— * 80% ale, wash — * D, 20 mins. -^ E, wash —>■ A, 2-5 mins. -^ F, 10-15 mins. — * wash 



— * G, if stain not sufficiently intense -^ H, 5 mins. — > wash — > balsam, via usual 



reagents 

 RECOMMENDED FOR: axis Cylinders and nerve endings. 



33.21 Romanes 1946 11025, 80:205 



REAGENTS REQUIRED: A. MS 33.1 Romanes 1946; B. AMS 21.1 Romanes 1946; C. 0.3% 



gold chloride in 2% acetic acid; D. 2% oxalic acid in 0.04% formaldehyde; E. 5% 



sodium thiosulfate 

 method: [paraffin sections] —* water —* A, 4-24 hrs. 58°C. —* rinse ^ B, 5 mins. -^ 



[repeat A — * B cycle if insufficientl.y stained] -^ wash — * C, 5 mins. -^ rinse — * D, 



10-15 mius. —> wash —* E, 5 mins. — > wash — > balsam, via usual reagents 



