MS 33.21-MS 33.22 METAL STAINS 585 



33.21 Schutz 1908 15058, 27:909 



UKAGKNTS uioQi ihkd: .1. 2% silvcp iiitrato; H. MS 33.1 Binlschowsky 1901; C. 8% neu- 

 tralized i'onaahlehyde; D. 1% acetic acid; E. 0.02% gold chloride; F. 5% sodium 

 thiosulfate 

 method: [sections by freezing technique of formaldehyde fixed material] —* wash — > A, 

 24 hrs. in dark — > wash -^ D, 10 mins. — » E, 30-49 mins. (till dark grey) -^ rinse -+ 

 F, 2 mins. — * balsam, via usual reagents 



33.21 Szatmari 1936 see MS 33.21 Gros-Schultze 1938 (note) 



33.21 Szepsenwol 1938 GG30, 120:089 



KKAOKNTs RixjiiRKi) I .4. F 0000.1030 Szcpseuwol 1935; B. 1% silver nitrate; C. 3% 



silver nitrate; 1). MS 33.1 Bielschowsky 1902 10, water 90 

 method: a. 10 to 20 days, 55°C. -^ running water, 24 to 48 hrs. — > J5, 1 to 3 days, 35°C., 



in dark — » C, 7 to 10 days, 35°C., in dark — > wash -^ D, several hrs. -^ wash —^ E, 



12 to 15 hrs. — > paraffin sections via cedar oil 

 note: This method is a slight modification of Szepsenwol 1937 (4285a, 14:168). 



33.21 Wallart 1935 4285a, 12:254 



reagents required: A. AMS 12.1 Wallart 1935; B. 2% silver nitrate; C. MS 33.1 



Wallart 1935; D. 4% neutralized formaldehyde 

 method: [3-5 mm. slices of formaldehyde material]-^ 4, 6 hrs. — > thorough wash — ► 



B. 5 days, 37°C. -^ rinse -^ C, 1-2 days -^ wash — > D, 12-18 hrs. —* wash — » [par- 

 affin sections] -^ AMS 22.1 treatment — > balsam, via usual reagents 



recommended for: nerves in organs rich in lipids. 



33.21 Weber 1944 4285a, 21 :45 



reagents required: A. AMS 12.1 Weber 1944; B. water 50, isopropanol 25, dioxane 25; 



C. 1% pyridine; D. 3% silver nitrate; E. MS 33.1 Weber 1944; F. AMS 21.1 Weber 

 1944 



method: [small fragments]—* A, 0°C., allow to rise to room temperature —>• 55°C., 1 

 month -+ B, wash, 30 mins. — > C, wash 12 hrs. -^ wash, till wash-water no longer 

 gives chloride test for silver -^ E, 24 hrs. — > F, 24 hrs. —* paraffin, via usual reagents 

 —y [5 fj. sections] — > balsam 



33.22 NEUROGLIA 



33.22 Achucarro 1911 3231, 1:139 



reagents required: A. sat. aq. sol. tannic acid; B. MS 33.1 Bielschowsky 1902 4, 



water 96; C. 4% formaldehyde 

 method: [sections, by freezing technique, of formaldehyde-fixed material]—*/!-^ 



warm till steaming -^ rinse — » B, 10 mins. — > C, 10 mins. -^ wash — * balsam, via 



usual reagents 

 recommended for: macroglia. 



33.22 Arcadi 1948 20540b, 23 :77 



reagents required: A. ammonia; B. 2% hydrobromic acid; C. MS 33.1 Arcadi 1948; 



D. 0.4% formaldehyde; E. 5% sodium thiosulfate 



method: [15 /x frozen sections] — > A, 100 ml. jar — > displace A with slow stream of water 

 over 24 hr. period — * A, fresh solution — » wash as before -^ A, fresh solution, 7 mins. 

 -* B, I hr., 38°C. -^ wash -» C, 30-45 mins. -* wash -^ D, 30 sees. -* wash -* E, 2 

 mins. -^ wash — > balsam, via usual reagents 



recommended for: oligodendria in material stored for many years in formaldehyde. 



33.22 Barker 1934 11977, 7:293 



reagents required: A. AMS 11.1 Cajal 1913; B. 0.5% ammonia; C. MS 33.1 del 



Rio-Hortega 1917a; D. 1% formaldehyde; E. 0.2% gold chloride; F. 5% sodium 



thiosulfate 

 method: [3 mm. slices of fresh tissue] — > A, 3 days -^ [25 m frozen sections] — * water — > 



A, 10 mins., 55°C. — > wash — + B, 2 mins. — > wash ^ C, 3 mins. — > rinse -^ D, I min. 



— > wash —> E, 5 mins. ^ i^, 1 min. — * wash — * balsam, via usual reagents 

 recommended for: microglia. 



