586 METHODS AND FORMULAS MS 33.22 



33.22 Belezky 1931 22575, 282:214 



REAGENTS REQUIRED: A. MS 33.1 Bclezky 1931; B. 4% neutralized formaldehyde 

 method: [sections by E 11.1 Belezky 1931] -* water, wash — > A, few sees. -^ wash — » 



B, till blackened — > balsam, via usual reagents 

 note: a may be diluted and used for a longer period. 



33.22 Bertrand and Guillain 1934 6630, 115:706 



REAGENTS REQUIRED: A. AMS 11.1 Bertrand and Guillain 1934; B. pyridine; C. 0.2% 



hydrofluoric acid; D. 5% sodium carbonate; E. MS 33.1 Bertrand and Guillain 1934; 



F. 2% formaldehyde; G. 0.2% gold chloride; H. 5% sodium thiosulfate 

 method: [fresh ganglia] -^ A, 4-7 days, renewed daily, 4°-5°C. — > wash — » 12 m frozen 



sections -^ B, 12-24 hrs. -> C, 1 hr., 37°C. -> wash -^ D, 4 hrs. -> E, till yellow -^ 



rinse —>■ F, till brown — * wash -^ G, till grey -^ H, 10 mins. — > wash ^ balsam, via 



usual reagents 

 recommended for: oligoglia in spinal and sympathetic ganglia. 



33.22 Bolsi test. 1933 Cajal and de Castro Cajal and de Castro 1933, 261 



REAGENTS REQUIRED: A. AMS 13.1 Bolsi 1933; B. MS 33.1 Cajal 1920; C. 2% formal- 

 dehyde; D. 0.2% gold chloride; E. 5% sodium thiosulfate 



method: [frozen sections of formaldehyde fi.\ed material] — > A, 30 mins., 50°C. — > wash 

 -^ B, 5-10 mins. — > wash -^ C, till reduced — > D, till violet — > £, 5 mins. — > balsam, 

 via usual reagents 



recommended for: macroglia. 



note: Cajal and de Castro {loc. cit.) suggest substitution of their AMS 13.1 for A above. 



33.22 Cajal 1920 test. 1933 ips. Cajal and de Castro 1933, 253 



reagents required: A. AMS 11.1 Cajal 1913; B. AMS 11.1 Cajal 1920; C. MS 33.1 



Cajal 1920b; Z). 6% neutralized formaldehyde; E. 0.2% gold chloride; F. 6% sodium 



thiosulfate 

 method: [small pieces] -^ A, 4 days -^ frozen sections — ^ B, 4 hrs., 37°C. -^ wash -^ C, 



40°-45°C., tQl deep brown -^ rinse — > D, few moments — > thorough wash — > E, 10-20 



mins. — > rinse -^ F, 5 mins. — > wash — » balsam, via clove oil and xylene 

 recommended for: macroglia. 



33.22 Cajal and de Castro 1933 see MS 33.22 Bolsi (1933) (note) 



33.22 Cone and Penfield test. 1929 Anderson Anderson 1929, 90 



REAGENTS REQUIRED: A. 0.1% ammonia; B. 5% hydrobromic acid; C. 5% sodium 



carbonate; D. MS 33.1 Cone and Penfield (1929); E. 0.5% formaldehyde; F. 0.2% 



gold chloride; G. 5% sodium thiosulfate 

 method: [20 n sections by freezing technique] -^ A, overnight -^ wash -^ B, I hr., 37°C. 



— > wash -^ C, 1 hr. — * D, 3-5 mins., or till brown —* E, 1 min. with agitation — ^ wash 



-^ F, till grey -^ G, 5 mins. — > balsam, via usual reagents 

 recommended for: microgUa. 



33.22 Gans test. 1933 Cajal and de Castro Cajal and de Castro 1933, 277 



reagents required: A. 2.5% ammonium bromide; B. 0.1% ammonia; C. MS 33.1 del 



Rio-Hortega 1921; D. 0.4% formaldehyde; E. 0.2% gold chloride; F. 5% thiosulfate 

 method: [25 m frozen sections of formaldehyde fixed material] — > A, 2 hrs., 37°C. — * B, 



quick rinse -^ C, 2 hrs. — > rinse — > D, 5 mins. —> E, 15 mins. -^ F, 5 mins. -^ balsam, 



via usual reagents 

 recommended for: microglia. 



33.22 Herrera 1932 test. 1933 Cajal and de Castro Cajal and de Castro 1933, 275 



reagents required: A. AMS 11.1 Herrera 1932; B. MS 33.1 Herrera 1932; C. 4% 



formaldehyde 

 method: [pieces of fresh tissue] —> A, 2 to 20 days — » 25 ^ frozen sections —> water -^ B, 



1 min. — > rinse — » C, 5 mins. — > wash — > balsam, via usual reagents 

 recommended for: microglia. 



33.22 Ingleby 1929 4349, 12:91 



reagents required: A. AMS 11.1 Cajal 1913; B. 10% tannic acid; C. 1% ammonia; 

 D. MS 33.1 da Fano 1919; E. 8% formaldehyde; F. 0.2% gold chloride; G. 5% sodium 

 thiosulfate 



