MS 33.22 METAL STAINS 587 



method: [slices of brain tissue] -> A, 2-4 days — » wash ^ [15 /x frozen sections] -* B, 5- 

 7 mins., 50°C., then cool 10 mins. —>■ C, till pliable -> D, till yellow-brown — > rinse —>■ 

 E, 1 min. -^ wash — > F, till groy — > G, 5 mins. — > wash — > balsam, via usual reagents 



note: F 5000.1010 Ingleby 1925 may be substituted for A if the time be reduced to 1 

 day. 



33.22 King 1937 1879,38:362 



REAGENTS REQUIRED: A. \% ammouia; B. 5% ammonium bromide; C. AMS 11.3 King 



1937; D. 3% sodium sulfite; E. MS 33.1 King 1937; F. 4% formaldehyde 

 method: [frozen sections of formaldehyde-fixed material]—* A, till required-^ B, 10 



mins., 50°C. -> C, 2 mins. -^ rinse -^^ D, 2-3 mins. -^ E, Z changes, 1 min. each -♦ 



rinse -^ F, till brown — > wash -^ balsam, via usual reagents 

 recommended for: microglia and oligodendroglia. 



33.22 McCarter 1940 608b, 16 :233 



REAGENTS REQUIRED: A. 1% ammouia; B. 4% hydrobromic acid; C. 5% sodium carbon- 

 ate; D. 5% ammonium alum; E. MS 33.1 del Rio-Hortega 1917a; F. 0.4% formal- 

 dehyde; G. 0.2% gold chloride; H. 5% sodium thiosulfate 

 method: [20 m frozen sections of formaldehyde material] -> A, few mins. to overnight, 

 according to age ^ B, 37°C., 1 hr. ^ wash ^ C -^ equal volume D, added to C 

 (ignore precipitate), leave 1 hr.-3 days -^ wash -^ E, 2-5 mins. — » F, 10 mins. -♦ 

 wash -^ G, till blue-grey -^ H, 5 mins. -^ balsam, via usual reagents 

 recommended for: oligodendria and microglia. 



33.22 Penfield 1928 608b, 4:153 



REAGENTS REQUIRED: A. AMS 11.1 Cajal 1913; B. 1% ammonia; C. 2% hydrobromic 



acid; D. 5% sodium carbonate; E. MS 33.1 del Rio-Hortega 1921 56, water 44; F. 1% 



formaldehyde; G. 0.2% gold chloride; H. 5% sodium thiosulfate 

 method: [fresh tissue] —> A, until wanted-* [sections, by freezing technique]-^ A, 5 



mins. -^ B, overnight -^ C, I hr., 38°C. -♦ wash -^ D, 1 hr. ^ rinse -^ E, 3-5 mins. 



or till light brown -^ rinse -^ F, I min. with agitation -^ wash G, till blue grey -^ H, 



5 mins. — * balsam, via usual reagents 



recommended for: microglia. 



33.22 Penfield 1924 3464, 47 :430 



REAGENTS required: A. AMS 11.1 Cajal 1913; B. MS 33.1 del Rio-Hortega 1921; C. 1% 



formaldehyde; D. 0.2% gold chloride; E. 5% sodium thiosulfate 

 method: [blocks of fre.sh tissue] -^ A, 4S hrs. -^ 95%) alcohol, 36-48 hrs. -> wash -^ sec- 

 tions, by freezing technique —>■ wash -* B, 30 mins. to 2 hrs. or until light brown -* 

 C, with agitation, 1 min. -^ D, till preferred color ^ ^, 1 min. -^ balsam, via usual 

 reagents 

 recommended for: oligodendroglia. 



33.22 Polak 1947 18794, 61 :508 



REAGENTS REQUIRED: AA % urauium acetate; B. MS 33.1 del Rio-Hortega 1917a; C. 1% 



formaldehyde; D. 0.2% gold chloride; E. 5% sodium thiosulfate 

 method: [5-10 m sections of formaldehyde material]—* water—* A, 24 hrs.—* B, till 



transparent, 15-60 sees. -^ rinse -* C, till brown -^ rinse ->■ D, till grey -* rinse -^ 



E, 5 mins. — > balsam, via usual reagents 



33.22 del Rio-Hortega 1916 21344, 14:181 



REAGENTS REQUIRED: A. 8% formaldehyde adjusted with ammonia to pH 8; B. 10% 

 tannic acid; C. 0.1%, ammonia; D. MS 33.1 Bielschowsky 1902 2, water 98 



method: Fix A, 2 to 3 days —>■ [sections by freezing technique] -^ B, heated till steam- 

 ing, 10 mins. -^ cool -^ C, till again flexible -^ D, till dark yellow -* rinse -^ A, 10 

 mins. — * balsam, via usual reagents 



note: del Rio-Hortega for A specifies the pH as that which gives a blue reaction with 

 litmus. 



recommended for: protoplasmic astrocytes. 



