588 METHODS AND FORMULAS MS 33.22 



33.22 del Rio-Hortega 1917 21344, 15:367 



REAGENTS REQUIRED: A. MS 33.1 del RIo-Hortega 1917b; B. 8% neutralized formalde- 

 hyde; C. 0.2% gold chloride; D. 5% sodium thiosulfate 



method: [sections by freezing technique of AMS 11.1 Cajal 1913 fixed material] -^ wash 

 — > A, 45°C., till black -^ rinse -^ B, 30 sees. -^ wash -^ C, till bluish -^ D, 5 mins. 

 — > wash — > balsam, via usual techniques 



RECOMMENDED FOR: astrocytes. 



note: Cajal and de Castro 1925 p. 255 cite this (without journal reference) as "Rio- 

 Hortega 1918" and recommend it for macroglia. 



33.22 del Rio-Hortega 1918 21344, 15:105 



reagents required: A. AMS 13.1 del Rio-Hortega 1910; B. 0.1% ammonia; C. MS 



33.1 del Rio-Hortega 1910 10, water 90; D. 8% neutralized formaldehyde 

 method: [sections, by freezing technique, of formol material] -^ A, 5 mins. 45°C. — > B, 

 till transparent — > C, 1st dish, 1 min. -^ C, 2nd dish, 1 min. -^ C, 3rd dish, till brown 

 -^ quick rinse -^ D, 30 sees. —^ wash -^ balsam, via usual techniques 

 recommended for: protoplasmic neuroglia. 



33.22 del Rio-Hortega 1919 3231, 9:68 



reagents required: A. 4% formaldehyde; B. MS 33.1 del Rio-Hortega 1917 26, 



water 74; C. 0.4% neutralized formaldehyde; D. 0.2% gold chloride; E. 5% sodium 



thiosulfate 

 method: [smears, or small pieces] — > A, 24 hrs. — > [section pieces by freezing technique] 



—^ wash —> B, 5 to 10 mins. -^ C, till reduced -^ D, 30 sees, to 1 min. -^ E, 1 min. 



-^ wash—* balsam, via usual reagents 



33.22 del RIo-Hortega 1921a 3232, 22:1 



reagents required: A. 4% formaldehyde adjusted to pH 8 with ammonia; B. MS 31.1 



del Rio-Hortega 1921; C. MS 33.1 del Rio-Hortega 1917a 29, water 71, pyridine 1; 



D. 0.4% formaldehyde 

 method: [sections by freezing technique of formaldehyde material] -^ ^4, 1 min. 50°C. -^ 



B, 50°C. till brown —^ wash — » C, 50°C. till dark brown -^ wash -^ D, b mins. — > 



balsam, via usual reagents 

 recommended for: oligodendria. 



33.22 del Rfo-Hortega 1921b 3232, 22:1 



reagents required: A. AMS 11.1 Cajal 1913; B. 0.1% ammonia; C. MS 33.1 del Rio- 

 Hortega 1921; D.4% neutralized formaldehyde; E. 0.2% gold chloride; /^. 5% sodium 

 thiosulfate 



method: Fix small pieces A, 2-3 days room temperature, then raise to 55°C. 10 mins. 

 — > [25 M sections by freezing technique] — > B, wash — * C, 3 mins. -^ D, 10 mins. -^ 

 wash — > E, 10 to 20 mins. -^ F, 5 mins. -^ balsam, via S 41.1 del Rio-Hortega (1938) 



recommended for: microglia. 



33.22 del Rio-Hortega 1923 21344, 21:95 



reagents required: A. 2% silver nitrate 100, pyridine 1.5; 5. 1% pyridine; C. MS 33.1 



del RIo-Hortega 1923 100, pyridine 1.5; D. 4% formaldehyde; E. 0.2% gold chloride; 



F. 5% sodium thiosulfate 

 method: [frozen sections of formaldehyde-fixed material] ^ wash —> A, 50°C. till 



brown some hrs. -^ B, wash -^ C, 50°C. till sepia -^ wash -^ D, 10 mins. -^ E, till 



violet —y F, 30 sees. —> blot on slide -^95% ale, dropped on slide -^ balsam, via 



S 41.1 del Rio-Hortega (1938) 

 recommended for: neuroglia in pineal. 



33.22 del Rio-Hortega 1927 3232, 27:199 



reagents required: A. AMS 11.1 Cajal 1913; B. 5% sodium sulfite or B. AMS 13.1 

 del Rio-Hortega 1927; C. MS 33.1 del Rio-Hortega 1927; D. 0.4% neutralized 

 formaldehyde; E. 0.2% gold chloride; F. 0.5% sodium thiosulfate 

 method: [fix small pieces A, 2-3 days room temperature then raise to 55°C. 10 mins.] — » 

 [25 n sections by freezing technique] — > B, either solution, several hours — > C, 1st 

 dish, 1 min. -^ C, 2nd dish, 2 mins. — > 1), 10 mins. -^ E, 10-20 mins. -^ F, 5 mins. — » 

 balsam, via S 41.1 del Rio-Hortega (1938) 

 RECOMMENDED FOR: microglia. 



