MS 33.22 METAL STAINS 580 



33.22 del Rfo-Hortega 1928 lest. 1933 Cajal and de Castro 



Cajal iuul <lc Castro 1<.)3:3, 280 

 REAGENTS REQUIRED: A. AM8 11.1 Cajal 1913; B. 0.1% aiiiiiioiiia; C. MS 33.1 del 

 Rlo-Hortega 1927; D. 0.4% formaldehyde; E. 0.2%, gold chloride; F. 5% sodium 

 thiosulfate 

 method: [pieces of fresh tissue] -+ ,4, 48 hrs. -^ A, 10 nuns. 45°-50°C. -^ [15 n to 20 n 

 frozen sections] —> B, 10 mins. — > wash — > C, 5-15 niins. —> D, 5 mins. —>■ E, 15 mins. 

 -^ F, 5 mins. — > balsam, via usual reagents 

 RECOMMENDED FOR: oligodendria. 



33.22 del Rio-Hortega 1932 lest. 1933 Cajal and de Castro 



Cajal aiul de Castro 1933, 2(13 

 REAGENTS REQUIRED: A. AMS 11.1 Cajal 1913: B. 0.1% ammonia; C. AMS 13.1 del 

 Rio-Hortega 1932; D. MS 31.1 del liio-Hortega 1932; E. MS 33.1 del Rio-Hortega 

 1932; F. 0.1% formaldehyde; G. 0.2% gold chloride; //. 5% sodium thiosulfate 

 method: [pieces of fresh tissue] -^ A, till required — > [frozen sections] — » B, wash — » C, 

 10 mins.-* D, 10-15 mins., 45°-50°C. -> E, till deep brown -^ 80% ale, wash ^ 

 95% ale, wash -^ F, 2-3 mins. — > C, till slate gray — > //^, 5 mins. -^ balsam, via 

 usual reagents 

 recommended for: gliob lasts. 



33.22 del Rio-Hortega test. 1933 Cajal and de Castro Cajal and de Castro 1933, 268 



reagents required: A. AMS 11.1 Cajal 1914; B. AMS 11.1 del Rio-Hortega 1923a; C. 



0.1% ammonia; D. MS 33.1 del Rio-Hortega 1933; E. 4% formaldehyde; F. 0.16% 



gold chloride; G. 5% sodium thiosulfate 

 method: [2-3 mm. slices fresh material]^ A, 2-3 days -^ B, 2-3 days, 25°-35°C. ^ 



[frozen sections] — » C, thorough wash -^ wash — > D, 45°-50°C., till deep straw 



colored — > wash — > E, few mins. — > F, 15 mins. — > G, 5 mins. — > balsam, via usual 



reagents 

 recommended for: gliosomes. 



33.22 del Rio-Hortega 1933 see MS 33.23 del Rio-Hortega 1933 



33.22 del Rio-Hortega 1929 3232, 30:1 



reagents required: A. 0.1 % anunonia; B. MS 33.1 del Rio-Hortega 1917 90, pyridine 

 10; C. 0.2% neutralized formaldehyde; D. 1% gold chloride; E. 5% sodium thiosulfate 



method: [sections by freezing techni(iue of material fixed in F 7000.1000 del Rio- 

 Hortega 1929] —>■ A, quick rinse —> wiish -^ B, at 55°C. till light brown -^ wash, 50% 

 ale. -^ C, 5 mins. -* D, 30 sees, to 1 min. —>^E,l min. — » wash -^ balsam via usual, 

 reagents 



33.22 Rodriguez test. 1933 Cajal and de Castro Cajal and de Castro 1933, 285 



reagents required: .4. AMS 11.1 Rodriguez 1933; B. equal parts A and 3% am- 

 monium oxalate; C. MS 33.1 Herrera 1932; D. O.A% formaldehyde 

 method: [pieces of fresh tissue] -^ A, 1-3 days -* [25 ^-30 m frozen sections] —* B, 24 



hrs. —> quick wash -^ C, 1 min. — > D, 5 mins. -* balsam, via usual reagents 

 recommended for: oligodendria. 



33.22 Weil and Davenport 1933 21458, 14:95 



reagents required: A. MS 33.1 Weil and Davenport 1933; B. 6% formaldehyde 

 method: [celloidin sections] —» water —> A, 15-20 sees. —+ rinse —> 5, until brown —> 



wash — > balsam, via usual reagents 

 recommended for: microglia. 



33.22 Winkler 1935 23430, 153:160 



reagents required: A. 2% hydrobromic acid; B. 2.5%, sodium carbonate; C. MS 33.1 

 del Rio-Hortega 1916 20, water 80; D. 8% formaldehyde 



method: [frozen sections of formaldehyde material] —* wash -* 0.3% alcohol, 2-8 hrs. 



• -^ wash -^ A, 14-24 hrs. -^ wa.sh -> B, 6-24 hrs. -* C, '2-2 mins. -* rinse -v D, till 

 reduced —> DS 13.11 May and Griinwald 1902, 24 hrs., if plasma cell staining re- 

 quired — > balsam, via usual reagents 



recommended for: microglia and plasma cells. 



