590 METHODS AND FORMULAS MS 33.23-MS 33.3 



33.23 OTHER NEUROLOGICAL METHODS 



33.23 Cajal test. 1933 ips. Cajal and de Castro 1933, 319 



REAGENTS REQUIRED: A. AMS 11.1 Cajal 1933a; B. MS 33.1 Cajal 19.33b; C. AMS21.1 



Cajal 1933 

 method: [pieces of fresh nerve] -> A, 24 hrs. -> thorough wash — » B, 48 hrs. — ♦ wash — » 



C, 6-12 hrs. — * teased preparations or celloidin sections 

 RECOMMENDED FOR: Schwann cells. 



33.23 Cajal test. 1933 ips. Cajal and de Castro 1933, 343 



REAGENTS REQUIRED: A. AMS 11.1 Cajal 1933b; B. water 100, MS 33.1 Bielschowsky 



1902 1; C. AMS 21.1 Cajal (1933) 

 method: [pieces of fresh nerve] -^ A, 24 hrs. -^ wash -^ B, several hrs. — * rinse — » C, 



several hrs. -^ teased preparations 

 RECOMMENDED FOR: peritubular connective sheath. 



33.3 Cytological Methods 



33.3 Fieandt and Sazen 1936 23632, 53:125 



REAGENTS REQUIRED; A. 4% neutralized formaldehyde; B. 3% silver nitrate; C. MS 33.1 

 del Rio-Hortega 1916; D. 0.5% acetic acid; E. 0.02% gold chloride; F. 5% sodium 

 thiosulfate 

 method: [20-30 m celloidin sections of material fixed in Y 7000.1010 Wittmaak (1910) 

 and decalcified by AF 21.1 Fieandt and Sazen 1936] — > [4-6 m paraffin sections neither 

 deparaffined nor attached to slide] -^ wash, 2 days ^ A, 3 days 37°C. — > wash, 24 

 hrs. -^ B, 31 2 days, 37°C. -^ rinse -* C, 6 hrs. —>■ D, 15 mins. -^ A, 10 mins. — * wash, 

 15 mins. -^ E, 2 hrs., 37°C. -* F, 2 mins. -^ wash, 12 hrs. — > [attach to slide] -^ 

 balsam, via usual reagents 

 recommended for: Golgi apparatus in cells of inner ear. 



33.3 Pritchard 1951 test. 1952 Cowdry Cowdry 1952, 212 



REAGENTS REQUIRED: A. MS 33.1 Foot 1927a 50, water 50, ammonia 0.1; B. 0.04% 



formaldehyde; C. 2% potassium ferricyanide 

 method: [sections of tissue fixed in F 7000.1000 Regaud 1910 or F 3700.1000 Helly 1903 

 and post-chromed 3 days in 3% potassium dichromate] -^ water —> A, 20 sees., with 

 constant agitation — ^ B, large volume, 20 sees. —* wash -^ C, till differentiated — > 

 wash -^ [counterstain, if desired] — > balsam, via usual reagents 

 note: Regaud fixation for mitochondria: Helly for Golgi. 



33.3 del Rio-Hortega 1916 21344, 14:181 



reagents required: A. 4% formaldehyde; B.3% tannic acid; C. 1% ammonia; D. MS 



33.1 del Rio-Hortega 1916 10, water 90 (3 dishes required); E. 1% gold chloride (2 



dishes required); F. 5% sodium thiosulfate 

 method: a, 10 days—* [sections by freezing technique] —> B, heated till steaming, 5 



mins. -^ C, till flexibility restored -^ D, 1st dish, 1 min. —»■ D, 2nd dish, 1 min. -» 



D, 3rd dish, 2 mins. -^ wash -^ E, 1st dish, 5 mins. -> E, 2nd dish, 55°C., 20 mins. -> 

 F, 2 mins. — > wash — > balsam, via usual techniques 



recommended for: mitochondria. 



33.3 del Rio-Hortega 1916 test. 1933 Cajal and de Castro 



Cajal and de Castro 1933, 211 

 reagents required: A. 3% tannin; B. 0.75% ammonia; C. MS 33.1 del Rio-Hortega 



1916 10, water 100; D. 0.2% gold chloride; E. 5% sodium thiosulfate 

 method: [5 M sections by freezing technique of formaldehyde-fLxed material] —> A, 5 

 mins., 50°-55°C. —>■ B, till flexibility and transparency regained -^ C, 1 min. in 3 

 successive baths -^ thorough wash -* D, 10 mins. 40°-45°C. -* E, 5 mins. -^ thor- 

 ough wash —> balsam, via usual reagents 

 recommended for: centrosomes in nerve cells. 



