MS 33.S-MS 33.41 METAL STAINS 591 



33.3 del Rio-Hortega test. 1933 Cajal and de Castro Cajal and de Castro 1933, 267 



REAGENTS REQUIRED: .4. AMS 11.1 Cajal 1914; B. AMS 11.1 del Rfo-Hortega 1933; C. 



0.1% ammonia; D. MS 33.1 del Rio-Hortega 1917; E. 0.2% formaldehyde; F. 0.16% 



gold chloride; G. 5% sodium thiosulfate 

 method: [2-3 mm. slices of fresh material] -^ A, 2-3 days — > B, 2-3 days, 25°-35°C. — > 



[frozen sections] — > C, till flexible — » wash — > D, 1-5 mins. — > wash — > E, 5 mins. — » 



F, 15 mins. -^ G, 5 mins. —* balsam, via usual reagents 

 recommended for: mitochondria. 



33.3 del Rio-Hortega 1925 3231, 25:34 



reagents required: A. AMS 11.1 Strong 1903; B. 1% ammonia; C. AMS 33.1 del 



Rio-Hortega 1921 56, water 44; D. 1% formaldehyde; E. 0.2% gold chloride; F. 5% 



sodium tliiosulfate 

 method; [small blocks]-* A, 2-8 days -^ [10 m sections by freezing technique]-* B, 



very brief wash -* wash -^ C, 5 mins. — » rinse, 15-20 sees, with very gentle agitation 



—* D, till grey — > wash -^ E, 15 mins. -^ F, 5 mins. — > balsam, via usual techniques 

 note: This formula can be modified to stain gliosomes differentially by (1) adding 1% 



pyridine to C and staining at 50% C till sections turn brown and (2) increasing to 



10% the concentration of D. All other steps are the same. 



33.3 del Rio-Hortega test. 1933 Cajal and de Castro Cajal and de Castro 1933, 269 



reagents required: A. AMS 11.1 del Rio-Hortega 1933b; B. 0.1% ammonia; C 2% 



silver nitrate; D. MS 33.1 del Rio-Hortega 1917; E. 0.4% formaldehyde; F. 0.16%, 



gold chloride; G. 5% sodium thiosulfate 

 method: [2-3 mm. slices of fresh tissue] ^ ^4, 3 or 4 days, on ice -^ [frozen sections] -* 



B, thorough wash — » wash — ♦ C, 15 mins. — > rinse — * Z), 1 min. — > rinse -^ E, 1 min. 



-^ F, 15 mins. -* (7, 5 mins. -* balsam, via usual reagents 

 recommended for: mitochondria in nerve cells. 



33.4 Histological Methods 



33.41 reticulum fibers 

 33.41 Amprino 1936 see MS 34.31 Amprino 1936 



33.41 Bensley and Bensley 1938a Bensley and Bensley 1938, 109 



REAGENTS REQUIRED: A. 1% potassiuiii permanganate; B. 5% oxalic acid; C. ADS 12.2 

 Lugol (1905) D. 5% sodium thiosulfate; E. sat. sol. tannic acid in 95% ale; F. 0.1% 

 ammonia; G. MS 33.1 Bensley and Bensley 1938a; H. 8% formaldehyde; /. 0.2%, 

 gold chloride 

 method: [sections] -^ water -^ A, 1-5 mins. -^ B, till bleached — * wash -^ C, 3-5 mins. 

 -^ wash -^ D, till bleached -* wash -^ E, 5 mins., 56°C. -^ F, rinse -^ G, 5-10 mins., 

 56°C. — > wash -^ H, 5 mins. -^ wash -^ I, till violet -* D, 5 mins. — * wash — > balsam, 

 via usual reagents 



33.41 Bensley and Bensley 1938b Bensley and Bensley 1938, 109 



REAGENTS REQUIRED: A. 2% silver nitrate; B. MS 33.1 Bensley and Bensley 1938b; C. 



8% neutralized formaldehyde 

 method: [sections] — > water — > A, overnight — + B, 15-30 mins. — > rinse -* C, 3 mins. — »• 

 balsam, via usual reagents 



33.41 del Carpio 1930 test. 1932 Findlay 11360, 62:155 



REAGENTS REQUIRED: A. 0.25% potassium permanganate; B. 5% oxalic acid; C. 2% 



silver nitrate; D. MS 33.1 del Carpio 1930; E. 4% neutralized formaldehyde; F. 0.2% 



gold chloride; G. 5% sodium thiosulfate 

 method: [sections, already dye-stained]-* water—* A, 15-20 mins. — > rinse -^ B, till 



bleached — > wash — > C, 24 hrs. — > rinse -^ D, 30 mins. —* double rinse — » E, 15 sees. 



— * wash -^ F, till gray — > wash — > G, 5-10 mins. -* wash — > balsam, via usual 



reagents 

 recommended for: reticular fibers in sections previously dye-stained. 



